HAF017). A specific band for CDC73 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDC73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for CDC73 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5508-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22637-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17817-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55257-25ul | |||
![Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Western-Blot-NB200-184-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Immunoprecipitation-NB200-184-img0007.jpg)
![Immunohistochemistry: CDC73/HRPT2 Antibody (JE66-42) [NBP3-32442] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CDC73/HRPT2 antibody (NBP3-32442) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32442) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32442_rabbit-cdc73-hrpt2-mab-je66-42-298202414304141.jpg "Immunohistochemistry: CDC73/HRPT2 Antibody (JE66-42) [NBP3-32442] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CDC73/HRPT2 antibody (NBP3-32442) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32442) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32442 at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: MCF7 cell lysate Lane 7: HEK-293 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32442 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32442_-western-blot-639204597796352212.jpg)
![Western Blot: CDC73/HRPT2 Antibody (1B8Q1) [NBP3-33442] - Western blot analysis of various lysates, using CDC73/HRPT2 Rabbit mAb at 1:800 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33442_rabbit-cdc73-hrpt2-mab-1b8q1-912202415143691.jpg "Western Blot: CDC73/HRPT2 Antibody (1B8Q1) [NBP3-33442] - Western blot analysis of various lysates, using CDC73/HRPT2 Rabbit mAb at 1:800 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.")
![Western Blot: CDC73/HRPT2 Antibody (SR2070) [NBP3-22637] - Western blot analysis using NBP3-22637 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22637_rabbit-cdc73-hrpt2-mab-sr2070-57202317215141.jpg "Western Blot: CDC73/HRPT2 Antibody (SR2070) [NBP3-22637] - Western blot analysis using NBP3-22637 in 293T cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP3-17817] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17817-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP3-17817] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP2-55257] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55257-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP2-55257] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.")
![Western Blot: CDC73/HRPT2 Antibody [NBP2-59257] - Western blot was performed on whole cell extracts (WCE) from drosophila larva and drosophila adults (Figure A) or on nuclear extracts from HeLa cells (HeLa NE, 20 ug) (Figure B) using the antibody against dCdc73 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Western-Blot-NBP2-59257-img0001.jpg "Western Blot: CDC73/HRPT2 Antibody [NBP2-59257] - Western blot was performed on whole cell extracts (WCE) from drosophila larva and drosophila adults (Figure A) or on nuclear extracts from HeLa cells (HeLa NE, 20 ug) (Figure B) using the antibody against dCdc73 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![Western Blot: CDC73/HRPT2 Antibody [NBP1-32972] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Western-Blot-NBP1-32972-img0004.jpg "Western Blot: CDC73/HRPT2 Antibody [NBP1-32972] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.")
