Angiopoietin-1 Antibodies

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin-1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Angiopoietin‑1 (Catalog # 923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF005). A specific band was detected for Angiopoietin-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for Angiopoietin-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin-1 Monoclonal Antibody (Catalog # MAB8220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />