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CD14 Antibodies

We offer CD14 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Cell Surface), Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Peptide ELISA, Sandwich ELISA, Western Blot. Each CD14 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD14 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Feline, Goat, Human, Llama, Mink, Monkey, Mouse, Mustelid, Porcine, Primate, Rabbit, Sheep. Use the list below to choose the CD14 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD14 polyclonal antibodies and browse our CD14 monoclonal antibody catalog.

Alternate Names for CD14 Antibodies

anti-CD14 antibody, anti-CD14 antigen antibody, anti-CD14 molecule antibody, anti-monocyte differentiation antigen CD14 antibody, anti-Myeloid cell-specific leucine-rich glycoprotein antibody

76 Results for "CD14" in Primary Antibodies

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NB100-77758

CD14 Antibody (M5E2) - BSA Free

(2 Reviews)

(14 Publications)

0.1 mg

NB100-77758

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.

Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Human peripheral blood monocytes stained with M5E2 APC

Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.

Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.

Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.

Dual RNAscope ISH-IHC: CD14 Antibody (M5E2) [NB100-77758] - TNFA mRNA (red) and CD14 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-TNFA and CD14 antibody (M5E2) at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Reactivity:

Hu, Po, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Po, Bv, Ca, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77758AF350
Alexa Fluor 405	NB100-77758AF405
Alexa Fluor 488	NB100-77758AF488
Alexa Fluor 532	NB100-77758AF532
Alexa Fluor 594	NB100-77758AF594
Alexa Fluor 647	NB100-77758AF647
Alexa Fluor 700	NB100-77758AF700
Alexa Fluor 750	NB100-77758AF750
Alexa Fluor Plus 405	NB100-77758AFP405
Alexa Fluor Plus 488	NB100-77758AFP488
Alexa Fluor Plus 555	NB100-77758AFP555
Alexa Fluor Plus 594	NB100-77758AFP594
Alexa Fluor Plus 647	NB100-77758AFP647
Alexa Fluor Plus 680	NB100-77758AFP680
Allophycocyanin	NB100-77758APC
Biotin	NB100-77758B
CoraFluor 1	NB100-77758CL1
DyLight 350	NB100-77758UV
DyLight 405	NB100-77758V
DyLight 488	NB100-77758G
DyLight 550	NB100-77758R
DyLight 594	NB100-77758DL594
DyLight 650	NB100-77758C
DyLight 680	NB100-77758FR
DyLight 755	NB100-77758IR
FITC	NB100-77759
FITC	NB100-77758F
HRP	NB100-77758H
Janelia Fluor 525	NB100-77758JF525
Janelia Fluor 549	NB100-77758JF549
Janelia Fluor 646	NB100-77758JF646
Janelia Fluor 669	NB100-77758JF669
PE	NB100-77758PE
PerCP	NB100-77758PCP
mFluor Violet 450 SE	NB100-77758MFV450
mFluor Violet 500 SE	NB100-77758MFV500
mFluor Violet 610 SE	NB100-77758MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-77758-0.5mg
BSA Free	NB100-77758SS
Azide and BSA Free	NBP2-80627

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MAB3832

CD14 Antibody (134620) [Unconjugated]

(26 Publications)

100 μg

MAB3832

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # <a class=

F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD14 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3832U-100UG
Alexa Fluor 405	FAB3832V-100UG
Alexa Fluor 488	FAB3832G-100UG
Alexa Fluor 532	FAB3832X
Alexa Fluor 594	FAB3832T-100UG
Alexa Fluor 647	FAB3832R-025
Alexa Fluor 647	FAB3832R-100
Alexa Fluor 700	FAB3832N-025
Alexa Fluor 700	FAB3832N-100
Alexa Fluor 750	FAB3832S-025
Alexa Fluor 750	FAB3832S-100
Alexa Fluor Plus 405	FAB3832AFP405-100UG
Alexa Fluor Plus 488	FAB3832AFP488-100UG
Alexa Fluor Plus 555	FAB3832AFP555-100UG
Alexa Fluor Plus 594	FAB3832AFP594-100UG
Alexa Fluor Plus 647	FAB3832AFP647-100UG
Alexa Fluor Plus 680	FAB3832AFP680-100UG
Allophycocyanin	FAB3832A-025
Allophycocyanin	FAB3832A-100
Allophycocyanin/Cy7	FAB3832APCCY7
Biotin	FAB3832B
CoraFluor 1	FAB3832CL1
DyLight 350	FAB3832D
DyLight 405	FAB3832E
DyLight 488	FAB3832K
DyLight 550	FAB3832L
DyLight 594	FAB3832M
DyLight 650	FAB3832W
DyLight 680	FAB3832Y
DyLight 755	FAB3832Z
Fluorescein	FAB3832F-100
Fluorescein	FAB3832F-025
HRP	FAB3832H
Janelia Fluor 525	FAB3832JF525
Janelia Fluor 549	FAB3832I
Janelia Fluor 646	FAB3832J
Janelia Fluor 669	FAB3832JF669
PE/Atto594	FAB3832PEATT594
PE/Cy5.5	FAB3832PECY55
PE/Cy7	FAB3832PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB3832C-025
PerCP (Peridinin-chlorophyll Protein Complex)	FAB3832C-100
Phycoerythrin	FAB3832P-025
Phycoerythrin	FAB3832P-100
mFluor Violet 450 SE	FAB3832MFV450
mFluor Violet 500 SE	FAB3832MFV500
mFluor Violet 610 SE	FAB3832MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3832V-100UG
Carrier Free	FAB3832C-100
Carrier Free	FAB3832U-100UG
Carrier Free	FAB3832P-025
Carrier Free	FAB3832P-100
Carrier Free	FAB3832N-025
Carrier Free	FAB3832N-100
Carrier Free	FAB3832F-025
Carrier Free	FAB3832F-100
Carrier Free	FAB3832C-025
Carrier Free	FAB3832A-025
Carrier Free	FAB3832T-100UG
Carrier Free	FAB3832A-100
Carrier Free	MAB3832-500
Carrier Free	FAB3832R-025
Carrier Free	FAB3832R-100
Carrier Free	FAB3832S-025
Carrier Free	FAB3832S-100
Carrier Free	MAB3832-SP
Carrier Free	FAB3832G-100UG

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NBP2-37291

CD14 Antibody (4B4F12) - BSA Free

(1 Review)

(1 Publication)

0.1 ml

NBP2-37291

Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.

<b>Biological Strategies Validation. </b>CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0010.jpg

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37291-0.025ml

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NBP2-67630

CD14 Antibody (SC69-02)

(3 Publications)

100 ul

NBP2-67630

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - analysis of CD14 on different lysates with Rabbit anti-CD14 antibody at 1/1,000 dilution.Lane 1: SW480-si NT cell lysateLane 2: SW480-si CD14 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 40 kDaObserved band size: 50-60 kDaExposure time: 7 seconds;ECL: merk4-20% SDS-PAGE gel.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB100-2807

CD14 Antibody

(4 Publications)

0.1 mg

NB100-2807

Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] - (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: Goat Polyclonal CD14 Antibody [NB100-2807] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-3991

Alt Catalog# IMG-3991

Conjugate:

Unconjugated

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NBP2-37295

CD14 Antibody (1H5D8) - BSA Free

(1 Review)

(1 Publication)

0.1 ml

NBP2-37295

Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) [NBP2-37295] - Human tonsil FFPE tissue. Antigen retrieval with EDTA buffer. Image from verified customer review.

Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)

Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded cervical cancer tissues using CD14 mouse mAb with DAB staining.

Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded colon tissues using CD14 mouse mAb with DAB staining.

ELISA: CD14 Antibody (1H5D8) [NBP2-37295] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37295-0.025ml

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NBP1-86168

CD14 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-86168

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86168 (A) shows similar protein distribution across tissues to independent antibody NBP1-86166 (B).

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.

<b>Independent Antibodies Validation. </b>Analysis using Anti-CD14 antibody NBP1-86168 (A) shows similar pattern to independent antibody NBP1-86166 (B).

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86168-25ul

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MAB4597

CD14 Antibody (433423)

(1 Review)

(2 Publications)

100 μg

MAB4597

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="Porcine peripheral blood mononuclear cells were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

<b>Biological Strategies Validation. </b>Equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # <a class=

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Po, Eq

Species Glossary

Reactivity: Po, Eq

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4597U-100UG
Alexa Fluor 405	FAB4597V-100UG
Alexa Fluor 488	FAB4597G-100UG
Alexa Fluor 532	FAB4597X
Alexa Fluor 594	FAB4597T-100UG
Alexa Fluor 647	FAB4597R-100UG
Alexa Fluor 700	FAB4597N-100UG
Alexa Fluor 750	FAB4597S-100UG
Alexa Fluor Plus 405	FAB4597AFP405-100UG
Alexa Fluor Plus 488	FAB4597AFP488-100UG
Alexa Fluor Plus 555	FAB4597AFP555-100UG
Alexa Fluor Plus 594	FAB4597AFP594-100UG
Alexa Fluor Plus 647	FAB4597AFP647-100UG
Alexa Fluor Plus 680	FAB4597AFP680-100UG
Allophycocyanin	FAB4597A
Allophycocyanin/Cy7	FAB4597APCCY7
Biotin	FAB4597B
CoraFluor 1	FAB4597CL1
DyLight 350	FAB4597D
DyLight 405	FAB4597E
DyLight 488	FAB4597K
DyLight 550	FAB4597L
DyLight 594	FAB4597M
DyLight 650	FAB4597W
DyLight 680	FAB4597Y
DyLight 755	FAB4597Z
FITC	FAB4597F
HRP	FAB4597H
Janelia Fluor 525	FAB4597JF525
Janelia Fluor 549	FAB4597I
Janelia Fluor 646	FAB4597J
Janelia Fluor 669	FAB4597JF669
PE	FAB4597P
PE/Atto594	FAB4597PEATT594
PE/Cy5.5	FAB4597PECY55
PE/Cy7	FAB4597PECY7
PerCP	FAB4597C
mFluor Violet 450 SE	FAB4597MFV450
mFluor Violet 500 SE	FAB4597MFV500
mFluor Violet 610 SE	FAB4597MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4597-SP
Carrier Free	FAB4597V-100UG
Carrier Free	FAB4597U-100UG
Carrier Free	FAB4597T-100UG
Carrier Free	FAB4597S-100UG
Carrier Free	FAB4597R-100UG
Carrier Free	FAB4597N-100UG
Carrier Free	FAB4597G-100UG

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AF982

CD14 Antibody [Unconjugated]

(5 Publications)

100 μg

AF982

Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CD14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF982) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CD14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF982) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>Glimepiride releases CD14 from RAW 264 cells. (A) The amounts of CD14 in RAW 264 cells treated for one hour with control medium (□) or glimepiride (■) as shown. Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (B) The amounts of CD14 in supernatants from RAW 264 cells treated for one hour with control medium (□) or glimepiride as shown (■). Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (C) Immunoblots showing the amounts of CD14, PrPC, CD55 and caveolin in extracts from RAW 264 cells treated for 1 hour with control medium (i) or 5 μM glimepiride (ii). (D) The amounts of CD14 in cells (□) or supernatants (■) from microglial cells treated for 1 hour with control medium, 5 μM glimepiride or 5 μM glipizide. Values are mean units CD14 ± SD, from triplicate experiments performed 3 times, n = 9. *Cellular CD14 significantly less than control cells. **supernatant CD14 significantly greater than control supernatants. (E) Blot showing the amounts of CD14 in supernatants from microglial cells treated with concentrations of glimepiride as shown for one hour. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24952384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF982U-100UG
Alexa Fluor 405	AF982V-100UG
Alexa Fluor 488	AF982G-100UG
Alexa Fluor 532	AF982X-100UG
Alexa Fluor 594	AF982T-100UG
Alexa Fluor 647	AF982R-100UG
Alexa Fluor 700	AF982N-100UG
Alexa Fluor 750	AF982S-100UG
Alexa Fluor Plus 405	AF982AFP405-100UG
Alexa Fluor Plus 488	AF982AFP488-100UG
Alexa Fluor Plus 555	AF982AFP555-100UG
Alexa Fluor Plus 594	AF982AFP594-100UG
Alexa Fluor Plus 647	AF982AFP647-100UG
Alexa Fluor Plus 680	AF982AFP680-100UG
Allophycocyanin	AF982A
Biotin	BAF982
CoraFluor 1	AF982CL1
DyLight 350	AF982D
DyLight 405	AF982E
DyLight 488	AF982K
DyLight 550	AF982L
DyLight 594	AF982M
DyLight 650	AF982W
DyLight 680	AF982Y
DyLight 755	AF982Z
FITC	AF982F
HRP	AF982H
Janelia Fluor 525	AF982JF525
Janelia Fluor 549	AF982I
Janelia Fluor 646	AF982J
Janelia Fluor 669	AF982JF669
PE	AF982P
PerCP	AF982C
mFluor Violet 450 SE	AF982MFV450
mFluor Violet 500 SE	AF982MFV500
mFluor Violet 610 SE	AF982MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF982-SP

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NBP1-86166

CD14 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-86166

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.

<b>Independent Antibodies Validation. </b>Analysis using Anti-CD14 antibody NBP1-86166 (A) shows similar pattern to independent antibody NBP1-86168 (B).

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86166-25ul

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MAB982

CD14 Antibody (159010) [Unconjugated]

(4 Publications)

500 μg

MAB982

Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CD14 Monoclonal Antibody (Catalog # MAB982) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CD14 Monoclonal Antibody (Catalog # MAB982) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD14 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD14 Monoclonal Antibody (Catalog # MAB982) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
CoraFluor 1	FAB982CL1
mFluor Violet 450 SE	FAB982MFV450
mFluor Violet 500 SE	FAB982MFV500
mFluor Violet 610 SE	FAB982MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB982-SP

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NBP2-25194

CD14 Antibody (RPA-M1)

0.1 mg

NBP2-25194

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Cell surface analysis of CD14 PerCP/Cy5.5 in 10^6 human monocytes using 10 ul of this antibody. Green represents PerCP-Cy5.5 conjugated isotype control; red represents anti-CD14 antibody. Cell surface flow kit was used for this test.

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the Azide Free version of NBP2-25194. Staining of CD14 in human PBMCs using anti-CD14 DyLight 405 conjugated antibody. Image from verified customer review.

Flow (Cell Surface): CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the FITC conjugate of NBP2-25194. Staining of CD14 in 10^6 human monocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD14 antibody.

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-25194. Staining of CD14 using CD14 monoclonal antibody. Surface staining of CD14 on 10^6 human monocytes using 10 ul of CD14 antibody. Green represents isotype control antibody; red represents CD14

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6350A

Alt Catalog# IMG-6350A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27215AF350
Alexa Fluor 405	NBP2-27215AF405
Alexa Fluor 488	NBP2-27215AF488
Alexa Fluor 532	NBP2-27215AF532
Alexa Fluor 594	NBP2-27215AF594
Alexa Fluor 647	NBP2-27215AF647
Alexa Fluor 700	NBP2-27215AF700
Alexa Fluor 750	NBP2-27215AF750
Alexa Fluor Plus 405	NBP2-27215AFP405
Alexa Fluor Plus 488	NBP2-27215AFP488
Alexa Fluor Plus 555	NBP2-27215AFP555
Alexa Fluor Plus 594	NBP2-27215AFP594
Alexa Fluor Plus 647	NBP2-27215AFP647
Alexa Fluor Plus 680	NBP2-27215AFP680
Allophycocyanin	NBP2-27215APC
Biotin	NBP2-27215B
DyLight 350	NBP2-27215UV
DyLight 405	NBP2-27215V
DyLight 488	NBP2-27215G
DyLight 550	NBP2-27215R
DyLight 594	NBP2-25194DL594
DyLight 650	NBP2-27215C
DyLight 680	NBP2-27215FR
DyLight 755	NBP2-27215IR
FITC	NBP2-27215F
Janelia Fluor 549	NBP2-27215JF549
Janelia Fluor 646	NBP2-27215JF646
PE	NBP2-27215PE
PerCP	NBP2-27215PCP
mFluor Violet 450 SE	NBP2-27215MFV450
mFluor Violet 500 SE	NBP2-27215MFV500
mFluor Violet 610 SE	NBP2-27215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-27215
Azide and BSA Free	NBP2-27215-25ug

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD14 Antibodies

CD14 Antibody (M5E2) - BSA Free

CD14 Antibody (134620) [Unconjugated]

CD14 Antibody (4B4F12) - BSA Free

CD14 Antibody (SC69-02)

CD14 Antibody

CD14 Antibody (1H5D8) - BSA Free

CD14 Antibody - BSA Free

CD14 Antibody (433423)

CD14 Antibody [Unconjugated]

CD14 Antibody - BSA Free

CD14 Antibody (159010) [Unconjugated]

CD14 Antibody (RPA-M1)