CD14 Antibodies

383-CD) was serially diluted and captured by Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3833) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 383-CD) was serially diluted and captured by Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3833) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human CD14 (Catalog # 383-CD) was serially diluted and captured by Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3833) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD14 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rabbit Anti-Human CD14, Monoclonal Antibody (Catalog #MAB11722) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF008). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for CD14 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for CD14 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of Human PBMC. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3831) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD14 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832R) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0111). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human CD14 Monoclonal Antibody (Catalog # MAB10461) followed by Goat anti-Rabbit IgG APC-conjugated Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

D8000C). IL-8 secre-tion elicited by Recombinant Human CD14 (Catalog # 383-CD, 10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human CD14 Monoclonal Antibody (Catalog # MAB10461). The ND₅₀ is typically 50-500 ng/mL in the presence of 15 ng/mL LPS and 0.5 ng/mL Recombinant Human LBP (Catalog # 870-LP)." class="big_lightbox"> D8000C). IL-8 secre-tion elicited by Recombinant Human CD14 (Catalog # 383-CD, 10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human CD14 Monoclonal Antibody (Catalog # MAB10461). The ND₅₀ is typically 50-500 ng/mL in the presence of 15 ng/mL LPS and 0.5 ng/mL Recombinant Human LBP (Catalog # 870-LP)." class="big_thumb" />

F0111). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human CD14 Monoclonal Antibody (Catalog # MAB11245) followed by Goat anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />