5'-Nucleotidase/CD73 Antibodies
We offer 5'-Nucleotidase/CD73 Antibodies for use in common research applications: ELISA, Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each 5'-Nucleotidase/CD73 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 5'-Nucleotidase/CD73 Antibodies can be used in a variety of model species: Equine, Human, Mouse, Porcine, Rat. Use the list below to choose the 5'-Nucleotidase/CD73 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 5'-Nucleotidase/CD73 polyclonal antibodies and browse our 5'-Nucleotidase/CD73 monoclonal antibody catalog.
Alternate Names for 5'-Nucleotidase/CD73 Antibodies
anti-5'-Nucleotidase/CD73 antibody, anti-NT5E antibody, anti-5' nucleotidase (CD73) antibody, anti-5'-NT antibody, anti-5'-nucleotidase, ecto (CD73) antibody, anti-CD73 antibody, anti-CD73 antigen antibody, anti-E5NT antibody, anti-EC 3.1.3.5 antibody, anti-ecto-5'-nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT55'-nucleotidase antibody, anti-NTE antibody, anti-Purine 5-Prime-Nucleotidase antibody
35 Results for "5-nucleotidase-cd73" in Primary Antibodies
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of bladder cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0005.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-ELISA-NBP2-37274-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Western-Blot-NBP3-14679-img0003.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of IMR-90 cells. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:100. HRP-conjugated goat anti-rabbit IgG Substrate DAB.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunocytochemistry-NBP3-14679-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human tonsil. Antibody: rabbit anti-CD73 recombinant monoclonal antibody used at 1:250. secondary: HRP- conjugated goat anti-rabbit igG](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (BLR054F) [NBP3-14679] - FFPE section of human head and neck squamous cell carcinoma. Rabbit anti-CD73 recombinant monoclonal antibody used at 1:500. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-BLR054F-Immunohistochemistry-NBP3-14679-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP1-32951-img0006.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - MDAMB231 xenograft, using CD73 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-32951-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32951_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405720.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Western-Blot-NBP2-89705-img0002.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Staining of human NT5E in human spleen with rabbit monoclonal antibody (1:5000).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89705-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (102) [NBP2-89706] - Staining of NT5E-3 in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NT5E-3 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-102-Immunocytochemistry-Immunofluorescence-NBP2-89706-img0001.jpg "Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (102) [NBP2-89706] - Staining of NT5E-3 in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NT5E-3 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm .")