5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." alt="Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca²⁺ionomycin then stained with Sheep Anti-Mouse SLAM/CD150 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_thumb" />

HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 10 ng/mL PMA and 200 ng/mL ionomycin for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." alt="Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca²⁺ionomycin then stained with Mouse SLAM/CD150 Monoclonal Antibody (Catalog # MAB4330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." class="big_thumb" />

HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_thumb" />

MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human SLAM/CD150 protein was serially diluted 2-fold and captured by Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />