MAB2198) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB2198) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human ADAMTS5 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog #
MAB2198) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB2198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human ADAMTS5 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB2198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![N/A ADAMTS5 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_ADAMTS5_DY219805_ELISA_2388.jpg "N/A ADAMTS5 [Biotin]")
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NBP2-15286-img0005.jpg "Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma, using ADAMTS5 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NBP2-15286-img0001.jpg)
![Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15286_rabbit-polyclonal-adamts5-antibody-western-blot-78202594139..png)
![Untreated (–) and treated (+) RMS 13 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15286_rabbit-polyclonal-adamts5-antibody-western-blot-7820259437..png)
![ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Samples were spiked with high concentrations of Human ADAMTS5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66444_human-adamts5-elisa-kit-colorimetric-132202416264315.jpg "ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Samples were spiked with high concentrations of Human ADAMTS5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66444_human-adamts5-elisa-kit-colorimetric-43202494159.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Colon, Carcinoma](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0006.jpg "Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Analysis of anti-ADAMTS5 antibody with cartilage at 3 ug/ ml.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0001.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Analysis of anti-ADAMTS5 antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0002.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0003.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Anti-ADAMTS5 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32001_rabbit-adamts5-mab-psh03-52-29202410334088.jpg "Immunocytochemistry/ Immunofluorescence: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Flow cytometric analysis of HepG2 cells labeling ADAMTS5.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32001, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32001_rabbit-adamts5-mab-psh03-52-392024884650.jpg)
![Immunohistochemistry: ADAMTS5 Antibody [NBP1-89247] - Staining of human lateral ventricle shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-NBP1-89247-img0003.jpg "Immunohistochemistry: ADAMTS5 Antibody [NBP1-89247] - Staining of human lateral ventricle shows strong cytoplasmic positivity in glial cells.")
![Immunocytochemistry/Immunofluorescence: ADAMTS5 Antibody [NBP2-55653] - Staining of human cell line RH-30 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55653-img0001.jpg "Immunocytochemistry/Immunofluorescence: ADAMTS5 Antibody [NBP2-55653] - Staining of human cell line RH-30 shows localization to nucleoplasm.")
