Jagged 1 Products

599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox" target="_blank">

599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox" target="_blank">

355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.00-6.00 ug/mL. " class="big_lightbox" target="_blank">