| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59382-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84846-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41371-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41373-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35497-20ul | |||
![Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Western-Blot-NB100-74357-img0002.jpg "Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0003.jpg)
![Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16353-img0002.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] -Mouse kidney.ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp2-16353_rabbit-polyclonal-epithelial-sodium-channel-alpha-antibody-immunohistochemistry-paraffin-592023111449..jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [N3C2], Internal (NBP2-16353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16353_rabbit-polyclonal-epithelial-sodium-channel-alpha-antibody-western-blot-261120251825500.jpg)
![ENaC Alpha antibody [N3C2], Internal detects ENaC Alpha protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal (NBP2-16353) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16353_rabbit-polyclonal-epithelial-sodium-channel-alpha-antibody-immunocytochemistry-immunofluorescence-2611202518345212.jpg)
![Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Western-Blot-NBP2-59382-img0003.jpg "Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.")
![Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Immunohistochemistry-NBP2-59382-img0005.jpg)
![Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Immunohistochemistry-NBP2-59382-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL3027] (NBP3-48763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48763_rabbit-epithelial-sodium-channel-alpha-mab-hl3027-western-blot-1411202518345921.jpg)
![Western Blot: epithelial Sodium Channel alpha Antibody - BSA Free [NBP3-45424] - PC-13 cells were subjected to SDS PAGE followed by western blot with (epithelial Sodium Channel alpha Antibody) at dilution of 1:600](http://images.novusbio.com/fullsize/nbp3-45424_rabbit-epithelial-sodium-channel-alpha-pab-bsa-free-28420251233173.jpg "Western Blot: epithelial Sodium Channel alpha Antibody - BSA Free [NBP3-45424] - PC-13 cells were subjected to SDS PAGE followed by western blot with (epithelial Sodium Channel alpha Antibody) at dilution of 1:600")
![Immunohistochemistry: epithelial Sodium Channel alpha Antibody - BSA Free [NBP3-45424] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (epithelial Sodium Channel alpha Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-45424_rabbit-epithelial-sodium-channel-alpha-pab-bsa-free-28420251233121.jpg)
![Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP1-84846] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-84846-img0004.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP1-84846] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP1-84846] - Staining of human gall bladder shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-84846-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP1-84846] - Staining in human gallbladder and skeletal muscle tissues using anti-SCNN1A antibody. Corresponding SCNN1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-84846-img0005.jpg)
![Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Western blot analysis of Rat kidney tissue lysates showing detection of epithelial Sodium Channel gamma protein using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:1000.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-Western-Blot-NBP3-18256-img0002.jpg "Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Western blot analysis of Rat kidney tissue lysates showing detection of epithelial Sodium Channel gamma protein using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:1000.")
![Immunohistochemistry: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Immunohistochemistry analysis using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-Immunohistochemistry-NBP3-18256-img0001.jpg)
![Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Western blot analysis of Mouse kidney cortex showing detection of epithelial Sodium Channel gamma protein using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.](http://images.novusbio.com/fullsize/epithelial Sodium Channel gamma Antibody-Western Blot-NBP3-18256-img0003.jpg)
![Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-Western-Blot-NBP2-41371-img0006.jpg "Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug")
![ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-ELISA-NBP2-41371-img0007.jpg)
![Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. Staining of Human kidney tissue. Courtesy of Per Svenningsen, PhD.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-5c2-BSA-Free-Immunohistochemistry-Paraffin-NBP2-41373-img0003.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. Staining of Human kidney tissue. Courtesy of Per Svenningsen, PhD.")
![Sandwich ELISA: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. The calibration curveof a sandwich assay using NBP2-41371 as the capture antibody and NBP2-41372B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-5c2-BSA-Free-Sandwich-ELISA-NBP2-41373-img0004.jpg)
![Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-35497] - Western blot analysis of various lysates using epithelial Sodium Channel gamma Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35497_rabbit-epithelial-sodium-channel-gamma-pab-1012202411433428.jpg "Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-35497] - Western blot analysis of various lysates using epithelial Sodium Channel gamma Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: epithelial Sodium Channel gamma Antibody [NBP3-35497] - Immunofluorescence analysis of paraffin-embedded mouse kidney using epithelial Sodium Channel gamma Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35497_rabbit-epithelial-sodium-channel-gamma-pab-101220241136019.jpg)
![Immunocytochemistry/ Immunofluorescence: epithelial Sodium Channel gamma Antibody [NBP3-35497] - Immunofluorescence analysis of paraffin-embedded human kidney cancer using epithelial Sodium Channel gamma Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35497_rabbit-epithelial-sodium-channel-gamma-pab-1012202411404066.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48756_rabbit-epithelial-sodium-channel-alpha-mab-hl2967-western-blot-141120251843561.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48756_rabbit-epithelial-sodium-channel-alpha-mab-hl2967-western-blot-141120251826020.jpg)
![Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody [NBP1-06075] - (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-06075-img0001.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody [NBP1-06075] - (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")