HAF017). Specific bands were detected for 4-1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF017). Specific bands were detected for 4-1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with recombinant mouse 4-1BB/Fc chimera. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF017). Specific bands were detected for 4-1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
F0107). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
MAB484). The ED
50 for this effect is typically 0.400–7.00 μg/mL." class="big_lightbox">
MAB484). The ED
50 for this effect is typically 0.400–7.00 μg/mL." class="big_thumb" />
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog #
MAB937) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog #
MAB937) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog #
MAB937) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog #
MAB937) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/ CD137-eGFP Fusion. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4-1BB/ TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody ([catalogNumber:HAF017). A specific band was detected for 4-1BB/ TNFRSF9/CD137-eGFP Fusion at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF017). Specific bands were detected for 4-1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with recombinant mouse 4-1BB/Fc chimera. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for 4-1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
FAB168A) and either (A) Rat Anti-Mouse 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." alt="Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Rat Anti-Mouse 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B)." alt="Human CD3+peripheral blood mononuclear cells were treated for 48 hours with 5 µg/mL PHA then stained with Mouse Anti-Human 4-1BB/TNFRSF9/CD137 Mono-clonal Antibody (Catalog # MAB838, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for 
F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of PHA stimulated cells. View our protocol for 
838-4B) was serially diluted and captured by Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) coated on a Clear Polystyrene Microplate (Catalog # 
838-4B) was serially diluted and captured by Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # 
838-4B) was serially diluted and captured by Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # 
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
FAB168A) and either (A) Goat Anti-Mouse 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (
CTS009) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for 
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (
MAB484). The ED50 for this effect is typically 0.400–7.00 μg/mL." class="big_thumb" />
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # 
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # ![N/A 4-1BB/TNFRSF9/CD137 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_41BB_DY838_ELISA_2242.jpg "N/A 4-1BB/TNFRSF9/CD137 [Biotin]")
FAB168A) and either (A) Rat Anti-Mouse 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) or (B) Rat IgG2AIsotype Control (Catalog # 
937-4B) was serially diluted and captured by Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) coated on a Clear Polystyrene Microplate (Catalog # ![N/A 4-1BB/TNFRSF9/CD137 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_41BB_DY937_ELISA_2270.jpg "N/A 4-1BB/TNFRSF9/CD137 [Biotin]")
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Western-Blot-NBP3-12029-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 4B4-1-1 (NBP3-12029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12029-img0002.jpg)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.](http://images.novusbio.com/fullsize/CD137-Antibody-Western-Blot-NBP2-31365-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.")
