MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="CHO Chinese hamster ovary cell line transfected with human Slit2 was stained with Rat Anti-Mouse Slit2 Monoclonal Antibody (Catalog # MAB5444, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5444-SP | |||
| Carrier Free | FAB5444V-100UG | |||
| Carrier Free | FAB5444U-100UG | |||
| Carrier Free | FAB5444T-100UG | |||
| Carrier Free | FAB5444S-100UG | |||
| Carrier Free | FAB5444R-100UG | |||
| Carrier Free | FAB5444N-100UG | |||
| Carrier Free | FAB5444G-100UG | |||
NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Slit2 was detected in immersion fixed frozen sections of E13.5 mouse hindlimb, shown localized to interdigital region (upper panel), using Sheep Anti-Mouse Slit2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5444) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5444-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 8616-SL-250 | |||
| Carrier Free | 8616-SL-01M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80742-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21803-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80743-25ul | |||
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg "Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Western blot analysis on different lysates with NBP3-32955 at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (30 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32955 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_-western-blot-639204597796446487.jpg)
![Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NB600-881-img0009.jpg "Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).")
![Immunohistochemistry: Slit2 Antibody [NB600-881] - Analysis of human breast carcinoma Tissue: Human prostate Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45” RT](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-NB600-881-img0005.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NB100-40807-img0001.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Western-Blot-NBP3-16194-img0002.jpg "Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Slit2 Antibody (1T5A3) [NBP3-16194] - Immunohistochemistry of paraffin-embedded rat ovary using Slit2 Rabbit mAb (NBP3-16194) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Immunohistochemistry-Paraffin-NBP3-16194-img0001.jpg)
![Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-21803_rabbit-slit2-mab-sr2034-57202316494277.jpg "Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.")
