PDGF R beta: Novus Biologicals

AF1042

PDGF R beta Antibody [Unconjugated]

100 μg

AF1042

HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

$Expression of sFlt1 in pericytes at the angiogenic front. a Maximum intensity projections of confocal images from P6 retinas of the Hey1-GFP transgenic reporter mouse model stained for GFP (green), PDGFR beta + (white) and IB4 (red). Images of the first row show enrichment of Hey1-GFP+, PDGFR beta + perivascular cells in the angiogenic front in comparison to mural cells covering the remodeling central plexus around veins (middle row) and arteries (bottom row). Note strong expression of Hey1-GFP reporter in arterial ECs (bottom row). Scale bar, 50 µm. b, Quantitation of Pdgfrb expression by qPCR in P6 PDGFR beta + retinal pericytes sorted based on GFP expression in comparison to whole-retina single-cell suspension (input). Note significant enrichment of Pdgfrb in both (GFP+ and GFP−) pericyte fractions and higher expression in the Hey1-GFP+ subset. Error bars, s.e.m. p-values, Kruskal–Wallis and Dunn’s multiple comparison test. NS, not statistically significant. c Quantitation of sFlt1 expression by qPCR in sorted P6 retinal pericytes in comparison to whole-retina single-cell suspension (input). Note significant enrichment of sFlt1 expression in Hey1-GFP+ pericytes in comparison to input and GFP- pericytes. Error bars, s.e.m. p-values, one-way ANOVA and Tukey’s multiple comparison test. NS, not statistically significant Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Genetic Strategies Validation. </b>VCAM1 is involved in the regulation of proliferation and morphological changes in oligodendrocytes.(a) Tissue lysates from 7-day-old NG2-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Ng2-Cre) or control (Ctrl) mouse spinal cords or 11-day-old whole brains were immunoblotted with an antibody against MBP, CNPase, VCAM1 or actin. Data are representative of three experiments. (b–d) Antibodies against Ki67 (green) and Olig2 (red) were used for co-staining in 2-day-old spinal cord cross sections. Data are representative. The scale bars indicate 200 μm. The number of Olig2+ cells per one square millimetre was counted. Data were evaluated using Student’s t-test (**P=0.000528; n=19 slices of two independent experiments). The percentage of Ki67+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00807; n=6 slices of two independent experiments). (e) Antibodies against Ki67 and NG2 were used for co-staining in 2-day-old spinal cord. The percentage of Ki67+ cells among the NG2+ cells per one square millimetre is shown. Data were evaluated using Student’s t-test (*P=0.0275; n=6 slices of two independent experiments). (f–h) Knock-down efficiencies of shRNAs were confirmed by immunoblotting. Data are representative of three experiments. Oligodendrocytes were transfected with VCAM1 shRNA or control, co-cultured with neurons for 2 days, and co-stained with antibodies against NF (green) and NG2 (red). Magnified images of the dotted boxes (i, attached; ii, aligned) are shown below. Data are representative. Scale bar, 100 μm. The percentage of NG2+ cells whose process tips or cell bodies were attached to axons or aligned along axons is shown. Data were evaluated using Student’s t-test (**P=0.00109 (attached cells) or P=0.00982 (aligned cells); n=6 areas of three experiments). (i,k) Oligodendrocytes were cultured on dishes coated with or without recombinant alpha 4 beta 1 integrin in a growth medium containing PDGF and bFGF. Cells were co-stained with an

Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). Note blunt appearance of the vessel front after VEGF-A injection but not for vehicle (PBS) control. Scale bar, 200 µm. b Quantitation of sprouts and filopodia at the front of the P6 vessel plexus after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. c PDGFR beta + (green) pericytes are unaffected by short-term VEGF-A administration, whereas VEGFR2 immunosignals (white) are increased in IB4+ (red) ECs (arrowheads). Images shown correspond to insets in a. Scale bar, 100 µm. d Quantitation of VEGFR2 immunosignals intensity in the peripheral plexus of P6 retinas after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. e Confocal images showing increased Esm1 immunostaining (white) in IB4+ (red) ECs in the peripheral plexus (arrowheads) after VEGF-A injection in P6 pups. Scale bar, 200 µm. f VEGF-A165 injection-mediated increase of Esm1 immunosignals (normalized to IB4+ EC area) in the peripheral capillary plexus but not at the edge of the angiogenic front in comparison to PBS-injected controls at P6. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant. g Short-term VEGF-A165 administration leads to clustering of Erg1+ (green) and IB4+ (red) ECs, as indicated, in thick sprout-like structures of P6 retinas. Panels in the center and on the right (scale bar, 20 µm) show higher magnification of the insets on the left (scale bar, 100 µm). Dashed lines in panels on the right outline IB4+ vessels. h Quantitation of EC density in the leading front vessel and emerging sprouts of the P6 angiogenic front after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubme

AF1042) at 1.7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of mouse embryo were probed for PDGFRb mRNA (ACD RNAScope Probe, catalog #411388; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse PDGFRb polyclonal antibody (R&D Systems catalog # <a class=

AF1042) at 1.7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. " class="big_thumb" />

PDGF autocrine loop is critical for VEGFR-2 down-expression and anti-VEGF resistance in GBM-associated ECs. a GBM tumor-derived ECs were treated with control IgG or antibody against PDGF-AA or PDGF-BB. Cell lysates were immunoblotted. b, c GBM tumor-derived ECs were transfected with control scrambled siRNA or siRNA targeting PDGFR-alpha and PDGFR-beta . b Cell lysates were immunoblotted. c Cell proliferation was determined (n = 3, mean ± SEM). d GBM tumor-derived ECs were treated with Ki8751 and crenolanib at different doses. Cell proliferation was determined 4 days after treatment. Inhibition rates were calculated and expressed as % of control cells Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30150753), licensed under a CC-BY license. Not internally tested by R&D Systems.

PDGF induces downregulation of VEGFR-2 expression in ECs. a, b Normal human brain microvascular ECs (#1 and #2 from adult brain and #3 from fetal brain) were treated with glioma-conditioned medium (glioma-CM). RNA was isolated and subjected to transcriptome analysis by RNA deep sequencing (RNA-seq). Left, heat map for expression of VEGF receptors. Right, fold changes of VEGFR-1, VEGFR-2, and VEGFR-3 (n = 3, mean ± SEM). b Shown are FPKM values of FSP-1 (n = 3). c Normal brain ECs were treated with glioma-CM or control normal medium. Cell lysates were immunoblotted. d Gene set analysis of upregulated pathways/genes identified by RNA-seq in glioma-CM-treated ECs. e ECs were isolated from GBM tumors or peri-tumor tissues of human patients or normal brains. Cell lysates were immunoblotted. Note: the lyates were also immunoblotted in Fig. 1c, and the same blot for GAPDH was shown. f Normal brain ECs were treated with 100 ng/ml PDGF-AA, PDGF-AB, or PDGF-BB. Cell lysates were immunoblotted Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30150753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Single-cell transcriptomic analysis of vessel organoids (VOrs).(A) UMAP plot showing the nine major cell types isolated from day (D) 40 VOrs. (B) Violin plots showing the expression value of the typical markers in each cluster. (C) Expression pattern of cell-type-specific markers in VOrs. Relative expression level is plotted from gray (low) to blue (high) colors. (D) Immunostaining of alpha SMA for representing the smooth muscle cells in VOrs. Scale bar, 10 μm. (E) Immunostaining of PDGFR beta for representing the pericytes in VOrs. Scale bar, 10 μm. (F) Immunostaining of microglia markers (IBA1, TREM2, TMEM119) and endothelial marker CD31 in VOrs at D40. Scale bar, 20 μm. (G) Violin plots showing the expression value of the venous marker EPHB4 and arterial marker DLL4 in endothelial cell (EC) clusters. (H) Expression pattern of arterial and venous markers in EC clusters. Relative expression level is plotted from gray to green (EPHB4) or red (DLL4) colors.Cell-type analysis for vessel organoids (VOrs) by scRNA-seq and immunostaining.(A) Correlation analysis of scRNA-seq data from two batches of VOr samples. (B) Proportions of cell types among all the cells from VOrs. (C) Heatmap showing the top five most enriched genes for each cell type. (D) Immunostaining of DLL4 for labeling the arterial endothelial cells in VOrs. Scale bar, 10 μm. (E) Immunostaining of EPHB4 for labeling the venous endothelial cells in VOrs. Scale bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35506651), licensed under a CC-BY license. Not internally tested by R&D Systems.

RFC1 protein is abundantly expressed in endothelial cells and pericytes of the retinal microvessels. A Ex-vivo labeling of 20 µm radial cryo-section from PFA fixated eyeballs of naive Swiss Albino mice with anti-RFC1 antibody (red). Abundant RFC1 immunopositivity is observed along ganglion cell layer (GCL), inner plexiform layer (IPL), outer plexiform layer (OPL) where retinal microvessels forms vascular horizontal vascular beds; as well as the retinal pigment epithelium (RPE). However, this preparation limited the observation of microvessels, hence inner BRB, as it commonly included microvessels circularly rather than longitudinally. Scale bar= 25 µm B RPE which is known to express RFC1 previously is well stained with anti-RFC1 antibody (red) as our positive control. C The microvessels constituting inner BRB form the deep vascular plexus of the retina were immunohistochemically labelled with anti-RFC1 antibody in PFA fixated whole-mount retinas. D Retinal microvessels which were obtained via retinal trypsin digestion method that allowed to get only microvessels (< 9 μm diameter) were immunofluorescently labeled with anti-RFC1 antibody (n = 6 retina; red). Nuclei were labeled with Hoechst 33258 (blue) in all the rows. E The endothelial marker CD31 (green) (n = 3). The “bump-on-a log” shaped pericyte body was positively stained with anti-RFC1 antibody, but negative for endothelial marker CD31. Hence, RFC1 staining was specific. F–H RFC1 (red) also colocalized with accepted pericyte markers NG2, CD13, PDGFR-beta (green) shown respectively (n = 3/marker). Nuclei were labeled with Hoechst 33258 (blue). Scale bars =10 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37328777), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL‐3 is specifically expressed in astrocytes at 14 dpi. (A) Immunofluorescent staining of CD31 (green), IL‐3 (red), and nuclei (blue) in sagittal sections at 14 dpi. (B) Immunofluorescent staining of PDGFR beta (green), IL‐3 (red), and nuclei (blue) in sagittal sections at 14 dpi. (C) Immunofluorescent staining of CD68 (green), IL‐3 (red), and nuclei (blue) in sagittal sections at 14 dpi. (D) Immunofluorescent staining of NG2 (green), IL‐3 (red), and nuclei (blue) in sagittal sections at 14 dpi. (E) Immunofluorescent staining of GFAP (green), IL‐3 (red), and nuclei (blue) in sagittal sections at 14 dpi. The region of interest (ROI) represents the boxed region on the left. Asterisks indicate the injured core. Scale bars: 200 μm (left panel) and 20 μm (right panel). n = 3 animals per group. (F) Quantification of the proportion of IL‐3+CD31+ cells in CD31+ cells, IL‐3+PDGFR beta + cells in PDGFR beta + cells, IL‐3+CD68+ cells in CD68+ cells, IL‐3+NG2+ cells in NG2+ cells, or IL‐3+GFAP+ cells in GFAP+ cells at 14 dpi. ND, no determined, **p < 0.01; ****p < 0.001 by one‐way ANOVA followed by Tukey's post hoc test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39697159), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1042U-100UG
Alexa Fluor 405	AF1042V-100UG
Alexa Fluor 488	AF1042G-100UG
Alexa Fluor 532	AF1042X-100UG
Alexa Fluor 594	AF1042T-100UG
Alexa Fluor 647	AF1042R-100UG
Alexa Fluor 700	AF1042N-100UG
Alexa Fluor 750	AF1042S-100UG
Alexa Fluor Plus 405	AF1042AFP405-100UG
Alexa Fluor Plus 488	AF1042AFP488-100UG
Alexa Fluor Plus 555	AF1042AFP555-100UG
Alexa Fluor Plus 594	AF1042AFP594-100UG
Alexa Fluor Plus 647	AF1042AFP647-100UG
Alexa Fluor Plus 680	AF1042AFP680-100UG
Allophycocyanin	AF1042A
Biotin	BAF1042
CoraFluor 1	AF1042CL1
DyLight 350	AF1042D
DyLight 405	AF1042E
DyLight 488	AF1042K
DyLight 550	AF1042L
DyLight 594	AF1042M
DyLight 650	AF1042W
DyLight 680	AF1042Y
DyLight 755	AF1042Z
FITC	AF1042F
HRP	AF1042H
Janelia Fluor 525	AF1042JF525
Janelia Fluor 549	AF1042I
Janelia Fluor 646	AF1042J
Janelia Fluor 669	AF1042JF669
PE	AF1042P
PerCP	AF1042C
mFluor Violet 450 SE	AF1042MFV450
mFluor Violet 500 SE	AF1042MFV500
mFluor Violet 610 SE	AF1042MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1042-SP

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AF385

PDGF R beta Antibody [Unconjugated]

(2 Reviews)

(64 Publications)

100 μg

AF385

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

385-PR) inhibits Recombinant Human PDGF-BB (220-BB) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND₅₀ is typically 10-40 µg/mL." class="big_lightbox">

Recombinant Human PDGF R beta Fc Chimera (<a class=

385-PR) inhibits Recombinant Human PDGF-BB (220-BB) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND₅₀ is typically 10-40 µg/mL." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

U87MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

U118MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

$Co-expression of EC marker and PDGFR beta after EPC-CM incubation.Co-expression of vWF and PDGFR beta was measured using dual color FACS analysis. HUVEC were kept in control medium containing only 1% FCS or EPC-CM for 24 h before the measurement. HUVEC incubated in control medium showed only a fractional amount of vWF+/PDGFR beta + cells (A). After EPC-CM exposure the proportion of vWF+/PDGFR beta + double positive population was significantly increased, suggesting a strong phenotype shift of endothelial cells towards PDGFR beta + (B). The addition of neutralizing antibody AF385 did not block the upreguation of the PDGFR beta by EPC-CM stimulation (C). However, such enhanced PDGFR beta expression could not be evoked by solely adding 100 ng/ml (D) or 100 pg/ml (E) rhPDGF-BB to the control medium. *, P<0.01 compared to controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014107), licensed under a CC-BY license. Not internally tested by R&D Systems.$

HAF017). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of SH‑SY5Y human neuroblastoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Pericyte soma on WM capillaries demonstrated by immunofluorescence. A–F. PDGFR‐ beta (red) and COL4 (green) immunofluorescence staining with nuclei (DAPI) (blue). A,B. Low‐ and high‐power images showing capillary segments (arrowheads) with overlapping PDGFR‐ beta (red), COL4 (green) and DAPI (blue). C. Same vessel segment as B with PDGFR‐ beta (red) and COL4 (green); D. COL4 (green) and DAPI (blue); E. PDGFR‐ beta (red) and DAPI (blue). F. Another capillary segment with PDGFR‐ beta (red), COL4 (green) and DAPI (blue) clearly showing pericyte cell body. G–J. Images taken by a confocal microscope showing pericyte cell bodies (arrows) with nuclear stain (DAPI). Capillaries and pericyte processes are revealed by COL4 and PDGFR‐ beta (red) immunoreactivities. Magnification bars: A = 50 µm, F, J = 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32705757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of MCT8 and OATP1C1 in blood vessels and brain barriers in the human and macaque basal ganglia and adjacent choroid plexus. (A) Representative brightfield photomicrograph shows immunostaining for MCT8 in the human putamen. Note that an MCT8 immunopositive signal is observed along the capillary wall (red arrowhead), fibers (green arrowhead), and “bump-on-a-log” morphology pericytelike cells (white arrowhead). (B–H) Representative confocal microscope compositions from multiple-stained sections for MCT8 (green), the endothelial marker UEA-I (red), and the vascular and pericyte biomarker PDGFR-beta (purple) in human and macaque caudate nucleus. Merged image (E,H) shows the colocalization of all signals. (B–E) Coexpression of MCT8, UEA-I, and PDGFR-beta is observed in a vessel, while coexpression of MCT8 and PDGFR-beta but not UEA-I is observed in a capillary-associated pericyte (white arrowheads) in humans. (F–H) Coexpression of MCT8 and PDGFR-beta in a vessel and pericytelike cells (white arrowheads) in macaques. Counterstaining with DAPI (blue) shows nuclei of all cells. (I,J) Representative brightfield photomicrographs show immunostaining for MCT8 (I) and OATP1C1 (J) in the macaque choroid plexus at the lateral ventricle. Black arrowheads point to ependymocytes. Cd: caudate nucleus, Put: putamen, PDGFR-beta : platelet-derived growth factor receptor-beta , UEA-I: Ulex europaeus agglutinin-I. Scale bar = 10 μm (A–H) and 50 μm (I,J). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF385U-100UG
Alexa Fluor 405	AF385V-100UG
Alexa Fluor 488	AF385G-100UG
Alexa Fluor 532	AF385X-100UG
Alexa Fluor 594	AF385T-100UG
Alexa Fluor 647	AF385R-100UG
Alexa Fluor 700	AF385N-100UG
Alexa Fluor 750	AF385S-100UG
Alexa Fluor Plus 405	AF385AFP405-100UG
Alexa Fluor Plus 488	AF385AFP488-100UG
Alexa Fluor Plus 555	AF385AFP555-100UG
Alexa Fluor Plus 594	AF385AFP594-100UG
Alexa Fluor Plus 647	AF385AFP647-100UG
Alexa Fluor Plus 680	AF385AFP680-100UG
Allophycocyanin	AF385A
Biotin	BAF385
CoraFluor 1	AF385CL1
DyLight 350	AF385D
DyLight 405	AF385E
DyLight 488	AF385K
DyLight 550	AF385L
DyLight 594	AF385M
DyLight 650	AF385W
DyLight 680	AF385Y
DyLight 755	AF385Z
FITC	AF385F
HRP	AF385H
Janelia Fluor 525	AF385JF525
Janelia Fluor 549	AF385I
Janelia Fluor 646	AF385J
Janelia Fluor 669	AF385JF669
PE	AF385P
PerCP	AF385C
mFluor Violet 450 SE	AF385MFV450
mFluor Violet 500 SE	AF385MFV500
mFluor Violet 610 SE	AF385MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF385-SP

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MAB1263

PDGF R beta Antibody (PR7212) [Unconjugated]

(23 Publications)

250 μg

MAB1263

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="MG-63 cells were stained with Mouse Anti-Human PDGF R beta Monoclonal Antibody (Catalog # MAB1263, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta , (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1263U-100UG
Alexa Fluor 405	FAB1263V-100UG
Alexa Fluor 488	FAB1263G-100UG
Alexa Fluor 532	FAB1263X
Alexa Fluor 594	FAB1263T-100UG
Alexa Fluor 647	FAB1263R-100UG
Alexa Fluor 700	FAB1263N-100UG
Alexa Fluor 750	FAB1263S-100UG
Alexa Fluor Plus 405	FAB1263AFP405-100UG
Alexa Fluor Plus 488	FAB1263AFP488-100UG
Alexa Fluor Plus 555	FAB1263AFP555-100UG
Alexa Fluor Plus 594	FAB1263AFP594-100UG
Alexa Fluor Plus 647	FAB1263AFP647-100UG
Alexa Fluor Plus 680	FAB1263AFP680-100UG
Allophycocyanin	FAB1263A
Allophycocyanin/Cy7	FAB1263APCCY7
Biotin	FAB1263B
CoraFluor 1	FAB1263CL1
DyLight 350	FAB1263D
DyLight 405	FAB1263E
DyLight 488	FAB1263K
DyLight 550	FAB1263L
DyLight 594	FAB1263M
DyLight 650	FAB1263W
DyLight 680	FAB1263Y
DyLight 755	FAB1263Z
Fluorescein	FAB1263F
HRP	FAB1263H
Janelia Fluor 525	FAB1263JF525
Janelia Fluor 549	FAB1263I
Janelia Fluor 646	FAB1263J
Janelia Fluor 669	FAB1263JF669
PE/Atto594	FAB1263PEATT594
PE/Cy5.5	FAB1263PECY55
PE/Cy7	FAB1263PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1263C
Phycoerythrin	FAB1263P
mFluor Violet 450 SE	FAB1263MFV450
mFluor Violet 500 SE	FAB1263MFV500
mFluor Violet 610 SE	FAB1263MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1263A
Carrier Free	FAB1263C
Carrier Free	FAB1263F
Carrier Free	FAB1263P
Carrier Free	MAB1263-SP
Carrier Free	FAB1263V-100UG
Carrier Free	FAB1263U-100UG
Carrier Free	FAB1263T-100UG
Carrier Free	FAB1263S-100UG
Carrier Free	FAB1263R-100UG
Carrier Free	FAB1263G-100UG
Carrier Free	FAB1263N-100UG

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NBP2-46361

PDGF R beta Antibody (OTI1E8)

0.1 ml

NBP2-46361

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73304AF350
Alexa Fluor 405	NBP2-73304AF405
Alexa Fluor 488	NBP2-73304AF488
Alexa Fluor 532	NBP2-73304AF532
Alexa Fluor 594	NBP2-73304AF594
Alexa Fluor 647	NBP2-73304AF647
Alexa Fluor 700	NBP2-73304AF700
Alexa Fluor 750	NBP2-73304AF750
Alexa Fluor Plus 405	NBP2-73304AFP405
Alexa Fluor Plus 488	NBP2-73304AFP488
Alexa Fluor Plus 555	NBP2-73304AFP555
Alexa Fluor Plus 594	NBP2-73304AFP594
Alexa Fluor Plus 647	NBP2-73304AFP647
Alexa Fluor Plus 680	NBP2-73304AFP680
Allophycocyanin	NBP2-73304APC
Biotin	NBP2-73304B
CoraFluor 1	NBP2-73304CL1
DyLight 350	NBP2-73304UV
DyLight 405	NBP2-73304V
DyLight 488	NBP2-73304G
DyLight 550	NBP2-73304R
DyLight 594	NBP2-73304DL594
DyLight 650	NBP2-73304C
DyLight 680	NBP2-73304FR
DyLight 755	NBP2-73304IR
FITC	NBP2-73304F
HRP	NBP2-73304H
Janelia Fluor 525	NBP2-73304JF525
Janelia Fluor 549	NBP2-73304JF549
Janelia Fluor 646	NBP2-73304JF646
Janelia Fluor 669	NBP2-73304JF669
PE	NBP2-73304PE
PerCP	NBP2-73304PCP
mFluor Violet 450 SE	NBP2-73304MFV450
mFluor Violet 500 SE	NBP2-73304MFV500
mFluor Violet 610 SE	NBP2-73304MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73304

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385-PR

Recombinant Human PDGF R beta Fc Chimera Protein

(1 Review)

(15 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	385-PR-100/CF

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NBP1-43349

PDGF R beta Antibody (APB5) - BSA Free

(1 Review)

(6 Publications)

0.1 mg

NBP1-43349

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43349AF350
Alexa Fluor 405	NBP1-43349AF405
Alexa Fluor 488	NBP1-43349AF488
Alexa Fluor 532	NBP1-43349AF532
Alexa Fluor 594	NBP1-43349AF594
Alexa Fluor 647	NBP1-43349AF647
Alexa Fluor 700	NBP1-43349AF700
Alexa Fluor 750	NBP1-43349AF750
Alexa Fluor Plus 405	NBP1-43349AFP405
Alexa Fluor Plus 488	NBP1-43349AFP488
Alexa Fluor Plus 555	NBP1-43349AFP555
Alexa Fluor Plus 594	NBP1-43349AFP594
Alexa Fluor Plus 647	NBP1-43349AFP647
Alexa Fluor Plus 680	NBP1-43349AFP680
Allophycocyanin	NBP1-43349APC
CoraFluor 1	NBP1-43349CL1
DyLight 350	NBP1-43349UV
DyLight 405	NBP1-43349V
DyLight 488	NBP1-43349G
DyLight 550	NBP1-43349R
DyLight 594	NBP1-43349DL594
DyLight 650	NBP1-43349C
DyLight 680	NBP1-43349FR
DyLight 755	NBP1-43349IR
FITC	NBP1-43349F
HRP	NBP1-43349H
Janelia Fluor 525	NBP1-43349JF525
Janelia Fluor 549	NBP1-43349JF549
Janelia Fluor 646	NBP1-43349JF646
Janelia Fluor 669	NBP1-43349JF669
PE	NBP1-43349PE
PerCP	NBP1-43349PCP
mFluor Violet 450 SE	NBP1-43349MFV450
mFluor Violet 500 SE	NBP1-43349MFV500
mFluor Violet 610 SE	NBP1-43349MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43349-0.05mg

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DYC1767-2

Human Phospho-PDGF R beta DuoSet IC ELISA, 2 Plate

(6 Publications)

1 Kit (for 2 Plates)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC1767E
Biotin	DYC1767-5

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DYC385-2

Human Total PDGF R beta DuoSet IC ELISA, 2 Plate

(3 Publications)

1 Kit (for 2 Plates)

DYC385-2

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC385-5

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AF1767

PDGF R beta [p Tyr751] Antibody

(1 Publication)

50 μg

AF1767

221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # <a class=

221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

221-AA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 274 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of CCD-1070Sk human foreskin fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA (Catalog # <a class=

221-AA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 274 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1767-SP

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MAB385

PDGF R beta Antibody (61520)

(3 Publications)

500 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB385U-100UG
Alexa Fluor 405	FAB385V-100UG
Alexa Fluor 488	FAB385G-100UG
Alexa Fluor 532	FAB385X-100UG
Alexa Fluor 594	FAB385T-100UG
Alexa Fluor 647	FAB385R-100UG
Alexa Fluor 700	FAB385N-100UG
Alexa Fluor 750	FAB385S-100UG
Alexa Fluor Plus 405	FAB385AFP405-100UG
Alexa Fluor Plus 488	FAB385AFP488-100UG
Alexa Fluor Plus 555	FAB385AFP555-100UG
Alexa Fluor Plus 594	FAB385AFP594-100UG
Alexa Fluor Plus 647	FAB385AFP647-100UG
Alexa Fluor Plus 680	FAB385AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB385-SP

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NBP1-19191

PDGF R beta Antibody (42G12) - BSA Free

(1 Publication)

0.1 mg

NBP1-19191

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191] - Human placenta at 20 ug/ml

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-19191-0.025mg

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1042-PR

Recombinant Mouse PDGF R beta Fc Chimera Protein, CF

(3 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Pdgfrb Products By Gene Name

PDGF R beta Antibody [Unconjugated]

PDGF R beta Antibody [Unconjugated]

PDGF R beta Antibody (PR7212) [Unconjugated]

PDGF R beta Antibody (OTI1E8)

Recombinant Human PDGF R beta Fc Chimera Protein

PDGF R beta Antibody (APB5) - BSA Free

Human Phospho-PDGF R beta DuoSet IC ELISA, 2 Plate

Human Total PDGF R beta DuoSet IC ELISA, 2 Plate

PDGF R beta [p Tyr751] Antibody

PDGF R beta Antibody (61520)

PDGF R beta Antibody (42G12) - BSA Free

Recombinant Mouse PDGF R beta Fc Chimera Protein, CF