| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90201-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49701SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65678SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71537 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26435-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17682-25ul | |||
![Western Blot: Alix Antibody [NBP1-90201] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDCD6IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP1-90201-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Alix Antibody [NBP1-90201] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDCD6IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-90201] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90201-img0008.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0014.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0011.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0012.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0013.jpg)
![Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP1-49701-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0005.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-49701] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- ALIX used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-49701-img0002.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on NIH3T3 cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0004.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on HeLa cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0006.jpg)
![Simple Western: Alix Antibody - BSA Free [NBP1-49701] - Alix Antibody [NBP1-49701] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Alix antibody (NBP1-49701) at approximately 106 kDa (as indicated) using 1:10 of Alix antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Alix-Antibody-Simple-Western-NBP1-49701-img0003.jpg)
![Western Blot: Alix Antibody (3A9) [NB100-65678] - B. Western blot for the exosomal marker Alix and Nef in cells and exosomes (exNef). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31344124) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alix-Antibody-3A9-Western-Blot-NB100-65678-img0002.jpg "Western Blot: Alix Antibody (3A9) [NB100-65678] - B. Western blot for the exosomal marker Alix and Nef in cells and exosomes (exNef). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31344124) licensed under a CC-BY license.")
![Western Blot: Alix Antibody (3A9) [NB100-65678] - Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (STAR13B)](http://images.novusbio.com/fullsize/Alix-Antibody-3A9-Western-Blot-NB100-65678-img0001.jpg)
![Western Blot: Alix Antibody (3A9) [NB100-65678] - Nef-containing exosomes deliver Nef to macrophages.A—Size distribution of the extracellular vesicles secreted by HEK293 cells determined by EM; Inset–EM micrograph of the vesicles; bar– 200 nm. B—Western blot for the exosomal marker Alix & Nef in cells & exosomes (exNef); C—Western blot for the indicated amounts of rNef & in a typical preparation of exNef (10 μg of exosomal protein); D, E–Time-course of exosome uptake quantitated by confocal microscopy; F–Time-course of exosome uptake quantitated by fluorimetry; percentage of added exosomes that was taken up is shown; G–Cells were incubated with exosomes for 48 h, excess exosomes was washed out & cells incubated for the indicated periods of time in exosome-free medum; retained fluorscence of the exosome stain PKH67 was assessed using confocal microscopy; H–Visualisation of Nef-GFP inside the cells after exposure to exNef-GFP (5 μg/ml of exosomal protein) after staining with anti-GFP antibody. Scale bars– 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31344124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65678_mouse-monoclonal-alix-antibody-3a9-31020241555231.jpg)
![Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Western-Blot-NBP2-46348-img0006.jpg "Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.")
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0005.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0001.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0002.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0003.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0004.jpg)
![Western Blot: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-71537-img0001.jpg "Western Blot: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.")
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0005.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0002.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0003.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0004.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody (3C4) [H00010015-M01] - Analysis of monoclonal antibody to PDCD6IP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Alix-Antibody-3C4-Immunocytochemistry-Immunofluorescence-H00010015-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: Alix Antibody (3C4) [H00010015-M01] - Analysis of monoclonal antibody to PDCD6IP on HeLa cell. Antibody concentration 10 ug/ml.")
![ELISA: Alix Antibody (3C4) [H00010015-M01] - Detection limit for recombinant GST tagged PDCD6IP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alix-Antibody-3C4-ELISA-H00010015-M01-img0006.jpg)
![Immunohistochemistry:[NBP3-28996]-Detection of human ALIX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ALIX used at a dilution of 1: 200. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-28996_rabbit-alix-pab-231202512881.jpg "Immunohistochemistry:[NBP3-28996]-Detection of human ALIX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ALIX used at a dilution of 1: 200. Detection: DAB")
![Immunohistochemistry:[NBP3-28996]-Alix Antibody](http://images.novusbio.com/fullsize/nbp3-28996_rabbit-alix-pab-31120250364046.jpg)
![Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate. All lanes: Alix Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/nbp3-26435_rabbit-alix-mab-7e9-282202411485823.jpg "Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate. All lanes: Alix Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa")
![Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] - Overlay histogram showing Jurkat cells stained with Alix Antibody (7E9) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26435_rabbit-alix-mab-7e9-282202414312767.jpg)
![Western Blot: Alix Antibody [NBP3-17682] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP3-17682-img0001.jpg "Western Blot: Alix Antibody [NBP3-17682] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Western Blot: Alix Overexpression Lysate (Adult Normal) [NBP2-10343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alix.](http://images.novusbio.com/fullsize/Alix-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10343-img0001.jpg "Western Blot: Alix Overexpression Lysate (Adult Normal) [NBP2-10343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alix.")
![Western Blot: Alix Antibody (S09-4B4) [NBP3-19564] - Western blot detection of Alix in HT1080 cell lysates using NBP3-19564 (1:1000 diluted). Predicted band size: 96KDa. Observed band size: 96KDa.](http://images.novusbio.com/fullsize/Alix-Antibody-S09-4B4-Western-Blot-NBP3-19564-img0001.jpg "Western Blot: Alix Antibody (S09-4B4) [NBP3-19564] - Western blot detection of Alix in HT1080 cell lysates using NBP3-19564 (1:1000 diluted). Predicted band size: 96KDa. Observed band size: 96KDa.")