| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77106-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72550 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92086-25ul | |||
![Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.](http://images.novusbio.com/fullsize/LXR-Antibody-Western-Blot-NB400-157-img0001.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.")
![Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/LXR-Antibody-Simple-Western-NB400-157-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0013.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0006.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody [NB300-612] - analysis of LXR alpha was done on HeLa. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 2.5% BSA for 30 minutes at room temperature. Cells were labeled with LXR alpha Rabbit Polyclonal Antibody (PA1330, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Flow-Cytometry-NB300-612-img0012.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody (2ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples was electrophoresed using 4-12 % Bis-Tris gel, Electrophoresis System and Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Blot 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0005.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue with LXR-A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunohistochemistry-Paraffin-NBP1-77106-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue lysate at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Rat liver tissue with LXR-A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunofluorescence-NBP1-77106-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419541563.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419313242.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419553048.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419432221.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Western-Blot-NBP2-46220-img0017.jpg "Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.")
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0016.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0001.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0002.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0003.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0005.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0007.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0008.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0009.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0010.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0011.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0012.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0013.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0014.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0015.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Western blot analysis of extracts of various cell lines, using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Western-Blot-NBP3-16304-img0007.jpg "Western Blot: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Western blot analysis of extracts of various cell lines, using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0006.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded mouse testis using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of Hep G2 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0005.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded rat ovary using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0001.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded human colon using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-5220251215493.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Confocal imaging of C2C12 cells using LXR alpha/NR1H3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-522025121547.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-52202511383726.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [LXR alpha/NR1H3] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-52202517304916.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [LXR alpha/NR1H3] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-5220251734586.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-72550-img0001.jpg "Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.")
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0016.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0002.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0003.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0005.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0007.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0008.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0009.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0010.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0011.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0012.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0013.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0014.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0015.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0017.jpg)
![Western Blot: LXR alpha/beta Antibody [NB100-1465] - Staining of Human Liver (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LXR-alpha-beta-Antibody-Western-Blot-NB100-1465-img0003.jpg "Western Blot: LXR alpha/beta Antibody [NB100-1465] - Staining of Human Liver (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/beta Antibody [NB100-1465] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/LXR-alpha-beta-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1465-img0004.jpg)
![Flow Cytometry: LXR alpha/beta Antibody [NB100-1465] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LXR-alpha-beta-Antibody-Flow-Cytometry-NB100-1465-img0005.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NBP1-92086] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-92086-img0004.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NBP1-92086] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody [NBP1-92086] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunohistochemistry-Paraffin-NBP1-92086-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (S07-6A4) [NBP3-19682] - Western blot detection of LXR alpha/NR1H3 in Rat Brain lysates using NBP3-19682 (1:1000 diluted). Predicted band size: 50kDa. Observed band size: 50kDa.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-S07-6A4-Western-Blot-NBP3-19682-img0002.jpg "Western Blot: LXR alpha/NR1H3 Antibody (S07-6A4) [NBP3-19682] - Western blot detection of LXR alpha/NR1H3 in Rat Brain lysates using NBP3-19682 (1:1000 diluted). Predicted band size: 50kDa. Observed band size: 50kDa.")
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (S07-6A4) [NBP3-19682] - Immunohistochemistry of LXR alpha/NR1H3 in paraffin-embedded Human breast cancer tissue using NBP3-19682 at dilution 1/50](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-S07-6A4-Immunohistochemistry-Paraffin-NBP3-19682-img0001.jpg)