| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-154SS | |||
| Azide and BSA Free | NBP2-80871 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37914-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21202-25ul | |||
| Azide and BSA Free | NBP3-44294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15123-25ul | |||
| Azide and BSA Free | NBP3-44353 | |||
![Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB300-155-img0004.jpg "Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).")
![Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-NB300-155-img0003.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27b-Immunohistochemistry-Paraffin-NB300-154-img0002.jpg "Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.")
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody [NBP2-37914] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-37914-img0006.jpg "Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody [NBP2-37914] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody [NBP2-37914] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-37914-img0004.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody [NBP2-37914] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-37914-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody [NBP2-37914] - Staining of human testis shows strong granular positivity in cytoplasm in Leydig cells.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-37914-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Neurofibromin 1 Antibody (CL11773) [NBP3-15123] - Staining of U-2 OS cells using the Anti-NF1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-CL11773-Immunocytochemistry-Immunofluorescence-NBP3-15123-img0001.jpg "Immunocytochemistry/Immunofluorescence: Neurofibromin 1 Antibody (CL11773) [NBP3-15123] - Staining of U-2 OS cells using the Anti-NF1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.")
![Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-29269] -Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NBP3-29269 (lot NBP3-29269-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies. For blotting immunoprecipitated NF1, NBP3-29269 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29269_rabbit-neurofibromin-1-pab-301220241952588.jpg "Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-29269] -Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NBP3-29269 (lot NBP3-29269-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies. For blotting immunoprecipitated NF1, NBP3-29269 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-30166] -Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NBP3-30166 (lot NBP3-30166-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies A304-931A and A300-140A. For blotting immunoprecipitated NF1, NBP3-30166 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30166_rabbit-neurofibromin-1-pab-301220242046549.jpg "Immunoprecipitation:Neurofibromin 1 AntibodyNBP3-30166] -Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NBP3-30166 (lot NBP3-30166-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies A304-931A and A300-140A. For blotting immunoprecipitated NF1, NBP3-30166 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")