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![Western Blot: Lumican Overexpression Lysate (Adult Normal) [NBP2-10821] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lumican.](http://images.novusbio.com/fullsize/Lumican-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10821-img0001.jpg "Western Blot: Lumican Overexpression Lysate (Adult Normal) [NBP2-10821] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lumican.")
![Flow Cytometry: Lumican Antibody (077) [NBP2-89941] - Analysis of Human LUM expression on HeLa cells . The cells were treated, stained with purified anti-Human LUM, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Lumican-Antibody-077-Flow-Cytometry-NBP2-89941-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Lumican Antibody (077) [NBP2-89941] - Analysis of Human LUM expression on HeLa cells . The cells were treated, stained with purified anti-Human LUM, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: Lumican Antibody (212) [NBP2-89943] - Staining of Human LUM in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89943 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89943_rabbit-monoclonal-lumican-antibody-212-23520251437135.png "Immunocytochemistry/Immunofluorescence: Lumican Antibody (212) [NBP2-89943] - Staining of Human LUM in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89943 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")