Il18 Products By Gene Name

HAF018). A specific band was detected for IL-18/IL-1F4 at approximately 22 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-18/IL-1F4 at approximately 22 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line, MCF 10A human breast epithelial cell line, A431 human epithelial carcinoma cell line, and HEK293T human embryonic kidney cell line (negative control cell line). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-18/IL-1F4 Propeptide Monoclonal Antibody (Catalog # MAB646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-18/IL-1F4 at approximately 22 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

MAB002, open histogram) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002, open histogram) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF018). A specific band was detected for IL-18/IL-1F4 at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH ) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-18/IL-1F4 at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH ) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-18/IL-1F4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF588) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." class="big_lightbox"> 210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." alt="In the presence of Recombinant Human TNF-alpha (Catalog # 210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB29241) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_lightbox"> 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." alt="In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_thumb" />