| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04307SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | IC4508G | |||
| Alexa Fluor 700 | IC4508N | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC4508G | |||
| Carrier Free | IC4508N | |||
| Carrier Free | AF4508-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71043 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14128-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21400-25ul | |||
![Western Blot: IRF5 Antibody [NB100-1092] - Western blot analysis of Human Spleen (A) and A549 cell lysate (B) + peptide (C), 35 ug protein in RIPA buffer. Antibody at 1 ug/mL. Detected by chemiluminescence](http://images.novusbio.com/fullsize/IRF5-Antibody-Western-Blot-NB100-1092-img0004.jpg "Western Blot: IRF5 Antibody [NB100-1092] - Western blot analysis of Human Spleen (A) and A549 cell lysate (B) + peptide (C), 35 ug protein in RIPA buffer. Antibody at 1 ug/mL. Detected by chemiluminescence")
![<b>Genetic Strategies Validation. </b>Western Blot: IRF5 Antibody [NB100-1092] - Endogenous IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs and lysates from same nucleofection run on multiple independent blots for comparative analysis of seven different commercially available alpha-IRF5 antibodies. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep31002), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IRF5-Antibody-Western-Blot-NB100-1092-img0003.jpg)
![Western Blot: IRF5 Antibody [NB100-1092] - Comparative analysis of IRF5 protein expression between immortalized lymphoid cell lines by immunoblot. Endogenous IRF5 protein expression in lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) was detecting by running multiple independent blots for the analysis of antibody specificity; seven different commercially available alpha-IRF5 antibodies were evaluated. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. beta-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep31002), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IRF5-Antibody-Western-Blot-NB100-1092-img0002.jpg)
![Western Blot: IRF5 Antibody [NB100-1092] - Overexpression of IRF5 in MCF-7 & MDA-MB-231 cells sensitizes them to DNA damage-induced growth inhibition. A. Endogenous IRF expression was analyzed by Western blot in transformed mammary epithelial cell lines. Levels of beta -actin are shown as loading controls. B. Western blot analysis of stable cell lines generated to overexpress retroviral pBIRF5. C. Cell survival was measured in MCF-7 & MDA-MB-231 pBabe cell lines by colony formation assay before & after treatment. Cells were treated with 0.1 or 1 μM Doxorubicin (Dox) or 2, 5 & 10 Gy gamma -IR. The number of colonies is plotted on the y-axis as percent of control; 100% represents the number of colonies in empty pBabe control lines. Data are expressed as mean ± SD of three independent experiments performed in duplicate. Statistical significance was determined by comparing the difference between colonies in pBabe versus pBIRF5 cell lines after each treatment; * denotes P 70% reduction of endogenous IRF5 proteins after normalization to beta -actin levels. B. Cells were exposed to 5 Gy IR or the same dose plus IFN-gamma (IR/ gamma ) for 24 h. Percent of Annexin V-FITC stained positive cells is shown in the upper & lower right-hand quadrants. Representative histogram plots from three independent experiments performed in duplicate are shown. C. Same as in B, except cells were exposed to 1 μM Dox or Dox & IFN-gamma for five hours. Percent of Annexin V-FITC stained positive cells compared to control is plotted on y-axis. Data are expressed as mean ± SD of three independent experiments performed in duplicate. Statistical significance was determined by comparing the difference between cells transfected with Lamin A/C siRNAs (12Asicon) & IRF5 siRNAs (12AsiIRF5) after each treatment; ** denotes P < 0.001. D. Cells were treated with the indicated doses of Dox or IR after siRNA transfection. Number of colonies is plotted on y-axis as percent of control. A total of 100% represents the number of colonies in control untreated 12Asicon cells. Data are expressed as mean ± SD of three independent experiments performed in duplicate. Statistical significance was determined by comparing the difference between colonies in 12Asicon versus 12AsiIRF5 cells after each treatment; * denotes P < 0.05. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1092_goat-polyclonal-irf5-antibody-imgenex-img-3525-31020241612691.jpg)
![Western Blot: IRF5 Antibody [NB100-1092] - Comparative analysis of IRF5 protein expression between immortalized lymphoid cell lines by immunoblot.(A) qRT-PCR analysis of IRF5 transcript expression in immortalized lymphoid cell lines. Data is presented as relative expression after normalization to beta -actin using the ∆∆Ct method. (B) Immunoblot analysis of endogenous IRF5 protein expression in immortalized cell lines. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. beta -actin levels & apparent molecular weight standards are shown as loading controls & for size comparison, respectively. (C) Experimental details for the comparative analysis of alpha -IRF5 antibody specificity by immunoblot analysis. (D) Same as in (B)except lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) were run on multiple independent blots for the analysis of antibody specificity; seven different commercially available alpha -IRF5 antibodies were evaluated. (E) Same as in (D) except antibodies were evaluated by IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs & lysates from same nucleofection run on multiple independent blots for comparative analysis. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data (except in (C)) are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep31002), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1092_goat-polyclonal-irf5-antibody-imgenex-img-3525-310202415525278.jpg)
![Western Blot: IRF5 Antibody [NB100-1092] - Overexpression of IRF5 in MCF-7 & MDA-MB-231 cells sensitizes them to DNA damage-induced growth inhibition. A. Endogenous IRF expression was analyzed by Western blot in transformed mammary epithelial cell lines. Levels of beta -actin are shown as loading controls. B. Western blot analysis of stable cell lines generated to overexpress retroviral pBIRF5. C. Cell survival was measured in MCF-7 & MDA-MB-231 pBabe cell lines by colony formation assay before & after treatment. Cells were treated with 0.1 or 1 μM Doxorubicin (Dox) or 2, 5 & 10 Gy gamma -IR. The number of colonies is plotted on the y-axis as percent of control; 100% represents the number of colonies in empty pBabe control lines. Data are expressed as mean ± SD of three independent experiments performed in duplicate. Statistical significance was determined by comparing the difference between colonies in pBabe versus pBIRF5 cell lines after each treatment; * denotes P < 0.05, ** P < 0.001. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1092_goat-polyclonal-irf5-antibody-imgenex-img-3525-31020241553200.jpg)
![Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/IRF5-Antibody-10T1-Western-Blot-NBP1-04307-img0005.jpg "Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/IRF5-Antibody-10T1-Immunocytochemistry-Immunofluorescence-NBP1-04307-img0008.jpg)
![Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/IRF5-Antibody-10T1-Immunohistochemistry-Paraffin-NBP1-04307-img0003.jpg)
![Flow (Intracellular): IRF5 Antibody (10T1) [NBP1-04307] - An intracellular stain was performed on THP-1 cells with IRF5 Antibody (10T1) NBP1-04307PE (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/IRF5-Antibody-10T1-Flow-Intracellular-NBP1-04307-img0010.jpg)
![Flow Cytometry: IRF5 Antibody (10T1) [NBP1-04307] - Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/IRF5-Antibody-10T1-Flow-Cytometry-NBP1-04307-img0009.jpg)
![Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/IRF5-Antibody-SD203-07-Western-Blot-NBP2-67742-img0004.jpg "Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IRF5-Antibody-SD203-07-Immunocytochemistry-Immunofluorescence-NBP2-67742-img0005.jpg)
![Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF5-Antibody-SD203-07-Immunohistochemistry-Paraffin-NBP2-67742-img0003.jpg)
![Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IRF5-Antibody-SD203-07-Flow-Cytometry-NBP2-67742-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IRF5-Antibody-SD203-07-Immunocytochemistry-Immunofluorescence-NBP2-67742-img0002.jpg)
![Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/IRF5-Antibody-1H6-Western-Blot-H00003663-M03-img0008.jpg "Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of monoclonal antibody to IRF5 on A-431 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/IRF5-Antibody-1H6-Immunocytochemistry-Immunofluorescence-H00003663-M03-img0005.jpg)
![Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/IRF5-Antibody-1H6-Western-Blot-H00003663-M03-img0007.jpg)
![ELISA: IRF5 Antibody (1H6) [H00003663-M03] - Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRF5-Antibody-1H6-ELISA-H00003663-M03-img0004.jpg)
![Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 mouse polyclonal antibody.](http://images.novusbio.com/fullsize/IRF5-Antibody-1H6-Immunoprecipitation-H00003663-M03-img0006.jpg)
![Western Blot: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5.](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Western-Blot-NBP2-45425-img0007.jpg "Western Blot: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5.")
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0006.jpg)
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0001.jpg)
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0002.jpg)
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0003.jpg)
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0004.jpg)
![Immunohistochemistry: IRF5 Antibody (1G7) [NBP2-45425] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-1G7-Immunohistochemistry-NBP2-45425-img0005.jpg)
![Western Blot: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5.](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Western-Blot-NBP2-71043-img0001.jpg "Western Blot: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5.")
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0006.jpg)
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0002.jpg)
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0003.jpg)
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0004.jpg)
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0005.jpg)
![Immunohistochemistry: IRF5 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71043] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IRF5-Antibody-OTI1G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71043-img0007.jpg)
![Western Blot: IRF5 Antibody (6L2B5) [NBP3-15343] - Western blot analysis of extracts of various cell lines, using IRF5 Rabbit mAb (NBP3-15343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/IRF5-Antibody-6L2B5-Western-Blot-NBP3-15343-img0001.jpg "Western Blot: IRF5 Antibody (6L2B5) [NBP3-15343] - Western blot analysis of extracts of various cell lines, using IRF5 Rabbit mAb (NBP3-15343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.")
![Immunoprecipitation: IRF5 Antibody (6L2B5) [NBP3-15343] - Immunoprecipitation analysis of 600ug extracts of Mouse thymus using 3ug IRF5 antibody (NBP3-15343). Western blot was performed from the immunoprecipitate using IRF5 antibody (NBP3-15343) at a dilition of 1:500.](http://images.novusbio.com/fullsize/IRF5-Antibody-6L2B5-Immunoprecipitation-NBP3-15343-img0002.jpg)
![Western Blot: IRF5 Antibody [NBP3-23445] - Western Blot analysis of Hela treated or untreated by LPS lysis, using NBP3-23445 at 1:1000 dilution. Secondary antibody was diluted at 1:10000](http://images.novusbio.com/fullsize/nbp3-23445_rabbit-irf5-pab-23420241446225.jpg "Western Blot: IRF5 Antibody [NBP3-23445] - Western Blot analysis of Hela treated or untreated by LPS lysis, using NBP3-23445 at 1:1000 dilution. Secondary antibody was diluted at 1:10000")
![Western Blot: IRF5 Antibody [NBP2-14128] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/IRF5-Antibody-Western-Blot-NBP2-14128-img0003.jpg "Western Blot: IRF5 Antibody [NBP2-14128] - Analysis in human cell line HDLM-2.")
