| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91660-25ul | |||
HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera-2 human testicular embryonic carcinoma cell line, and mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2909-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91659-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47996-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54376-100ug | |||
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91660-img0004.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human pancreas shows no postivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0014.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249374918.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249383191.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-212024940384.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249393565.jpg)
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse kidney.</br>Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-immunohistochemistry-paraffin-2611202518413916.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-western-blot-26112025198367.jpg)
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human kidney.</br>Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-immunohistochemistry-paraffin-261120251838151.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Western-Blot-NBP3-19565-img0003.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunocytochemistry-Immunofluorescence-NBP3-19565-img0001.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunohistochemistry of Alkaline Phosphatase, Tissue Non-Specific in paraffin-embedded Human lung cancer tissue using NBP3-19565 at dilution 1/50](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunohistochemistry-Paraffin-NBP3-19565-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19565_rb-alkaline-phosphatase-tissue-non-specific-mab-s08-6h0-11320251555912.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human pancreas shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0014.jpg)
![Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp3-09106_mouse-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-w8b2b10-flow-cytometry-1212202415932..jpg "Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).")
![Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Anti-Hu TNAP purified antibody (concentration in sample 0.3 ug/ml, GAM APC, red-filled histogram) binds specifically to TNAP on subpopulation of HeLa cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).](http://images.novusbio.com/fullsize/antibody/nbp3-09106_mouse-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-w8b2b10-flow-cytometry-12122024151035..jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-V17-1-Immunohistochemistry-Paraffin-NBP2-44962-img0002.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0008.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0005.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0006.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0007.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0007.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - ALPL monoclonal antibody (M01), clone 4H1 Western Blot analysis of ALPL expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0001.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0005.jpg)
![ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-ELISA-H00000249-M01-img0006.jpg)
![ELISA: Mouse Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP3-18017] - Samples were spiked with high concentrations of Mouse Alkaline Phosphatase, Liver/Bone/Kidney and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18017_mouse-alkaline-phosphatase-liver-bone-kidney-elisa-kit-132202416211379.jpg "ELISA: Mouse Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP3-18017] - Samples were spiked with high concentrations of Mouse Alkaline Phosphatase, Liver/Bone/Kidney and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP3-18017] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18017_mouse-alkaline-phosphatase-liver-bone-kidney-elisa-kit-4320249163359.jpg)
![ELISA: Monkey Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP2-69934] - Samples were spiked with high concentrations of Monkey Alkaline Phosphatase, Liver/Bone/Kidney and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69934_monkey-alkaline-phosphatase-liver-bone-kidney-elisa-kit-13220241617340.jpg)
![ELISA: Monkey Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP2-69934] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69934_monkey-alkaline-phosphatase-liver-bone-kidney-elisa-kit-432024915810.jpg)
