| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74462 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38460-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35805-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38880-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22607-50ul | |||
![Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Western-Blot-NBP2-00597-img0004.jpg "Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.")
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Immunocytochemistry-Immunofluorescence-NBP2-00597-img0006.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0003.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0001.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0002.jpg)
![Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Western-Blot-NB100-57094-img0002.jpg "Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NB100-57094-img0003.jpg)
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38460-img0005.jpg "Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38460] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NBP2-38460-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)
![Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TBC1D4-[p-Ser588]-Antibody-Western-Blot-NBP3-05451-img0001.jpg "Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38880] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NBP2-38880-img0002.jpg "Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38880] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.")
![Western Blot: TBC1D4 Overexpression Lysate (Adult Normal) [NBP2-07277] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07277-img0001.jpg "Western Blot: TBC1D4 Overexpression Lysate (Adult Normal) [NBP2-07277] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TBC1D4.")
![Immunohistochemistry: TBC1D4 Antibody - BSA Free [NBP2-81935] - Immunohistochemistry of TBC1D4 in human prostate tissue with TBC1D4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81935_rabbit-polyclonal-tbc1d4-antibody-2711202420171775.jpg "Immunohistochemistry: TBC1D4 Antibody - BSA Free [NBP2-81935] - Immunohistochemistry of TBC1D4 in human prostate tissue with TBC1D4 antibody at 10 ug/mL.")
![Western Blot: TBC1D4 [p Thr 642] Antibody (SR1272) [NBP3-22607] - Western blot analysis using NBP3-22607 in 293T treated with insulin cell lysate.](http://images.novusbio.com/fullsize/nbp3-22607_rabbit-tbc1d4-p-thr-642-mab-sr1272-5720231531231.jpg "Western Blot: TBC1D4 [p Thr 642] Antibody (SR1272) [NBP3-22607] - Western blot analysis using NBP3-22607 in 293T treated with insulin cell lysate.")