CD44: Novus Biologicals

BBA10

CD44 Antibody (2C5) [Unconjugated] - s Pan Specific

(5 Reviews)

200 μg

BBA10

HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PC-3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 156 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of MDA‑MB‑231 human breast cancer parental cell line and CD44 knockout MDA-MB-231 cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated secondary antibody. A specific band was detected for CD44 at approximately 81.5 kDa (as indicated) in the parental MDA-MB-231 cell line, but is not detectable in knockout MDA-MB-231 cell line. Primary antibody concentration used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

$MDA‑MB‑231 human breast cancer cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) pre-coupled to Dynabeads Protein G. Immunoprecipitated CD44 was detected in Western Blot with a rabbit CD44 antibody used at 1/3000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

MDA‑MB‑231 human breast cancer parental cell line WT and CD44 MDA-MB-231 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 2 μg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

MDA‑MB‑231 human breast cancer parental cell line WT and CD44 KO cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed in a 1:1 ratio, fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells were stained with Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) and a secondary antibody. Antibody staining was quantified, and representative images showing the staining intensity in the KO population (pink histogram, dashed line) compared to the WT cells (green histogram, solid line) are presented. Histograms with dotted lines represent secondary antibody-only controls in both WT and KO cells. Primary antibody concentration: 1 μg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Differential localization of Kv1.3 and KCa3.1 in migrating T cells.A. Distribution of Kv1.3 and KCa3.1 at the uropod. T cells were transiently transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CD44 antibody (uropod; red) without permeabilization. Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. B. Distribution of Kv1.3 and KCa3.1 at the leading-edge. T cells, transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CXCR-4 antibody (leading-edge; red) without permeabilization, were analyzed by confocal microscopy. Colocalization between the two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. C. Correlation coefficients for KCa3.1 and Kv1.3 localization in the uropod (U) and leading-edge (L). The data are the average of n = 15 cells for KCa3.1 at the U and n = 8 at the L, and n = 16 for Kv1.3 at the U and n = 11 at the U from 2 healthy individuals. Statistical significance was established by one way ANOVA. D. Localization of native Kv1.3 in the leading-edge. T cells from one healthy individual were fixed and stained with extracellular anti-Kv1.3 antibody (green) together with antibodies either against CD44 (red; left) or CXCR-4 (red, right). Yellow colors in the merge images indicate strong correlation. Scale bar = 5 µm. E. Average Correlation coefficients of native Kv1.3 with the leading-edge (L) (n = 9) and the uropod (U) markers (n = 11). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043859), licensed under a CC-BY license. Not internally tested by R&D Systems.

A-B Expression of CD44st, CD44s, CD44v6 mRNA and miR-138-5p in breast cancer cell lines. A Semi-quantitative PCR agarose electrophoresis plots of different cell lines B Quantitative PCR results of different cell lines Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36964570), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	BBA10U-100UG
Alexa Fluor 405	BBA10V-100UG
Alexa Fluor 488	BBA10G-100UG
Alexa Fluor 532	BBA10X-100UG
Alexa Fluor 594	BBA10T-100UG
Alexa Fluor 647	BBA10R-100UG
Alexa Fluor 700	BBA10N-100UG
Alexa Fluor 750	BBA10S-100UG
Alexa Fluor Plus 405	BBA10AFP405-100UG
Alexa Fluor Plus 488	BBA10AFP488-100UG
Alexa Fluor Plus 555	BBA10AFP555-100UG
Alexa Fluor Plus 594	BBA10AFP594-100UG
Alexa Fluor Plus 647	BBA10AFP647-100UG
Alexa Fluor Plus 680	BBA10AFP680-100UG
Allophycocyanin	FAB4948A
Biotin	BBA10B
CoraFluor 1	BBA10CL1
DyLight 350	BBA10D
DyLight 405	BBA10E
DyLight 488	BBA10K
DyLight 550	BBA10L
DyLight 594	BBA10M
DyLight 650	BBA10W
DyLight 680	BBA10Y
DyLight 755	BBA10Z
FITC	BBA10F
HRP	BBA10H
Janelia Fluor 525	BBA10JF525
Janelia Fluor 549	BBA10I
Janelia Fluor 646	BBA10J
Janelia Fluor 669	BBA10JF669
PerCP	BBA10C
Phycoerythrin	FAB4948P
mFluor Violet 450 SE	BBA10MFV450
mFluor Violet 500 SE	BBA10MFV500
mFluor Violet 610 SE	BBA10MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB4948P
Carrier Free	FAB4948A

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NBP1-47386

CD44 Antibody (8E2F3) - BSA Free

(1 Review)

(22 Publications)

0.1 ml

NBP1-47386

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

CD44-Antibody-8E2F3-BSA-Free-Western-Blot-NBP1-47386-img0021.jpg

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).

Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

$Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP1-47386B) at 2 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

U-251 MG human glioblastoma cell line was stained with Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-47386AF647, blue histogram) or matched control antibody (orange histogram).

CD44 (8E2F3) was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

CD44 (8E2F3) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP1-47386B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

$MDA‑MB‑231 human breast cancer cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of CD44 Antibody (8E2F3) - BSA Free (Catalog # NBP1-47386) pre-coupled to Dynabeads Protein G. Immunoprecipitated CD44 was detected in Western Blot with a rabbit CD44 antibody used at 1/3000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

<b>Genetic Strategies Validation. </b>MDA‑MB‑231 human breast cancer parental cell line WT and CD44 MDA-MB-231 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with CD44 Antibody (8E2F3) - BSA Free (Catalog # NBP1-47386) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Analysis of a FFPE tissue section of human skin using 1:200 dilution of CD44 (8E2F3) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Reactivity:

Hu, Mu, Rb(-), Pm, Ca, Eq

Species Glossary

Reactivity: Hu, Mu, Rb(-), Pm, Ca, Eq

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-47386AF350
Alexa Fluor 405	NBP1-47386AF405
Alexa Fluor 488	NBP1-47386AF488
Alexa Fluor 532	NBP1-47386AF532
Alexa Fluor 594	NBP1-47386AF594
Alexa Fluor 647	NBP1-47386AF647
Alexa Fluor 700	NBP1-47386AF700
Alexa Fluor 750	NBP1-47386AF750
Alexa Fluor Plus 405	NBP1-47386AFP405
Alexa Fluor Plus 488	NBP1-47386AFP488
Alexa Fluor Plus 555	NBP1-47386AFP555
Alexa Fluor Plus 594	NBP1-47386AFP594
Alexa Fluor Plus 647	NBP1-47386AFP647
Alexa Fluor Plus 680	NBP1-47386AFP680
Allophycocyanin	NBP1-47386APC
Allophycocyanin/Cy7	NBP1-47386APCCY7
Biotin	NBP1-47386B
CoraFluor 1	NBP1-47386CL1
DyLight 350	NBP1-47386UV
DyLight 405	NBP1-47386V
DyLight 488	NBP1-47386G
DyLight 550	NBP1-47386R
DyLight 594	NBP1-47386DL594
DyLight 650	NBP1-47386C
DyLight 680	NBP1-47386FR
DyLight 755	NBP1-47386IR
FITC	NBP1-47386F
HRP	NBP1-47386H
Janelia Fluor 525	NBP1-47386JF525
Janelia Fluor 549	NBP1-47386JF549
Janelia Fluor 646	NBP1-47386JF646
Janelia Fluor 669	NBP1-47386JF669
PE	NBP1-47386PE
PE/Atto594	NBP1-47386PEATT594
PE/Cy5.5	NBP1-47386PECY55
PE/Cy7	NBP1-47386PECY7
PerCP	NBP1-47386PCP
mFluor Violet 450 SE	NBP1-47386MFV450
mFluor Violet 500 SE	NBP1-47386MFV500
mFluor Violet 610 SE	NBP1-47386MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-47386SS
Azide and BSA Free	NBP2-80650

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BBA13

CD44 Antibody (2F10) [Unconjugated] - v6

(34 Publications)

200 μg

BBA13

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_lightbox">

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." alt="Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD44 was detected in immersion fixed A431 human epithelial carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD44 was detected in immersion fixed paraffin-embedded sections of human colon adenocarcinoma tissue using Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, and TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunochemical staining of selected biomarkers in early gastric cancer tissues.(A) E-cadherin (B) a1 catenin (C) p53 (D) EZH (E) CD44v6. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159424), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Knockdown of CD44v6 with siRNA transfection in YCC-2 cell line and overexpression of CD44v6 with plasmid transfection in MKN-28 cell line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	BBA13U-100UG
Alexa Fluor 405	BBA13V-100UG
Alexa Fluor 488	FAB3660G
Alexa Fluor 532	BBA13X-100UG
Alexa Fluor 594	BBA13T-100UG
Alexa Fluor 647	BBA13R-100UG
Alexa Fluor 700	BBA13N-100UG
Alexa Fluor 750	BBA13S-100UG
Alexa Fluor Plus 405	BBA13AFP405-100UG
Alexa Fluor Plus 488	BBA13AFP488-100UG
Alexa Fluor Plus 555	BBA13AFP555-100UG
Alexa Fluor Plus 594	BBA13AFP594-100UG
Alexa Fluor Plus 647	BBA13AFP647-100UG
Alexa Fluor Plus 680	BBA13AFP680-100UG
Allophycocyanin	FAB3660A
Biotin	BBA13B
CoraFluor 1	BBA13CL1
DyLight 350	BBA13D
DyLight 405	BBA13E
DyLight 488	BBA13K
DyLight 550	BBA13L
DyLight 594	BBA13M
DyLight 650	BBA13W
DyLight 680	BBA13Y
DyLight 755	BBA13Z
FITC	BBA13F
HRP	BBA13H
Janelia Fluor 525	BBA13JF525
Janelia Fluor 549	BBA13I
Janelia Fluor 646	BBA13J
Janelia Fluor 669	BBA13JF669
PerCP	BBA13C
Phycoerythrin	FAB3660P
mFluor Violet 450 SE	BBA13MFV450
mFluor Violet 500 SE	BBA13MFV500
mFluor Violet 610 SE	BBA13MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3660P
Carrier Free	FAB3660G
Carrier Free	FAB3660A

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NB500-481

CD44 Antibody (MEM-263) - BSA Free

(1 Review)

(10 Publications)

0.1 mg

NB500-481

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.

CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg

Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.

Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. (a)-(b). CD44+ cells also express CK18 as determined by flow cytometry (a) and western blot analysis (b). Flow cytometer is representative of the eight evaluated clones. EOC stem cells, determined by either CD44 (c and d) or Ck18 (e and f) expression, are found in clusters surrounded by CD44− or Ck18− negative cancer cells.

Reactivity:

Hu, Po, Ca

Species Glossary

Reactivity: Hu, Po, Ca

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-481AF350
Alexa Fluor 405	NB500-481AF405
Alexa Fluor 488	NB500-481AF488
Alexa Fluor 532	NB500-481AF532
Alexa Fluor 594	NB500-481AF594
Alexa Fluor 647	NB500-481AF647
Alexa Fluor 700	NB500-481AF700
Alexa Fluor 750	NB500-481AF750
Alexa Fluor Plus 405	NB500-481AFP405
Alexa Fluor Plus 488	NB500-481AFP488
Alexa Fluor Plus 555	NB500-481AFP555
Alexa Fluor Plus 594	NB500-481AFP594
Alexa Fluor Plus 647	NB500-481AFP647
Alexa Fluor Plus 680	NB500-481AFP680
Allophycocyanin	NB500-481APC
Allophycocyanin/Cy7	NB500-481APCCY7
Biotin	NB500-481B
CoraFluor 1	NB500-481CL1
DyLight 350	NB500-481UV
DyLight 405	NB500-481V
DyLight 488	NB500-481G
DyLight 550	NB500-481R
DyLight 594	NB500-481DL594
DyLight 650	NB500-481C
DyLight 680	NB500-481FR
DyLight 755	NB500-481IR
FITC	NBP1-42789
HRP	NB500-481H
Janelia Fluor 525	NB500-481JF525
Janelia Fluor 549	NB500-481JF549
Janelia Fluor 646	NB500-481JF646
Janelia Fluor 669	NB500-481JF669
PE	NB500-481PE
PE/Atto594	NB500-481PEATT594
PE/Cy5.5	NB500-481PECY55
PE/Cy7	NB500-481PECY7
PerCP	NB500-481PCP
mFluor Violet 450 SE	NB500-481MFV450
mFluor Violet 500 SE	NB500-481MFV500
mFluor Violet 610 SE	NB500-481MFV610

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NBP1-31488

CD44 Antibody - BSA Free

(13 Publications)

100 ul

NBP1-31488

Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-31488] - Analysis of methanol-fixed HeLa, using CD44 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human cervical carcinoma. CD44 stained by CD44 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human endometrial carcinoma. CD44 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human pancreatic tumor, using CD44 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-31488] - CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.</br>Sample: A431 cells were fixed in ice-cold MeOH for 5 min.</br>Green: CD44 stained by CD44 antibody (NBP1-31488) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Genetic Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CMTM6 regulates Wnt signaling via the ENO-1/AKT/GSK3 beta / beta -catenin axis.(A) CMTM6 was overexpressed in chemoresistant cells stably expressing NTShRNA or CMTM6ShRNA#2, and immunoblotting (n = 3) was performed with indicated antibodies. (B) Lysates were isolated from cisplatin-resistant lines stably transfected with NtSh and ENO1Sh and subjected to immunoblotting (n = 3) for indicated antibodies. (C) Myr AKT (constitutively active AKT) was overexpressed in chemoresistant cells stably expressing NTShRNA or CMTM6ShRNA#2, and immunoblotting (n = 3) was performed with indicated antibodies. (D) Lysates were isolated from LY294002-treated cells and vehicle control, and immunoblotting (n = 3) was performed using indicated antibodies. (E) mRNA was isolated from LY294002-treated cells and vehicle control, and relative mRNA (fold change) expression (n = 3) of indicated genes was analyzed by qRT-PCR in the indicated cell. Two-way ANOVA. (F) IHC of beta -catenin and target genes in tumor of PDX model. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33434185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Rb

Species Glossary

Reactivity: Hu, Rt, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF6127

CD44 Antibody [Unconjugated]

(1 Review)

(13 Publications)

100 μg

AF6127

HAF016). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse spleen tissue, mouse lymph node tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse spleen tissue, mouse lymph node tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox">

Mouse splenocytes were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox">

Rat splenocytes were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_lightbox">

Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or isotype control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_thumb" />

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_lightbox">

Equine peripheral blood mononuclear cells (PBMCs) were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or isotype control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_thumb" />

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD44 was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox">

CD44 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 169 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Reactivity:

Mu, Rt, Po, Eq

Species Glossary

Reactivity: Mu, Rt, Po, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6127U-100UG
Alexa Fluor 405	AF6127V-100UG
Alexa Fluor 488	AF6127G-100UG
Alexa Fluor 532	AF6127X-100UG
Alexa Fluor 594	AF6127T-100UG
Alexa Fluor 647	AF6127R-100UG
Alexa Fluor 700	AF6127N-100UG
Alexa Fluor 750	AF6127S-100UG
Alexa Fluor Plus 405	AF6127AFP405-100UG
Alexa Fluor Plus 488	AF6127AFP488-100UG
Alexa Fluor Plus 555	AF6127AFP555-100UG
Alexa Fluor Plus 594	AF6127AFP594-100UG
Alexa Fluor Plus 647	AF6127AFP647-100UG
Alexa Fluor Plus 680	AF6127AFP680-100UG
Allophycocyanin	AF6127A
Biotin	AF6127B
CoraFluor 1	AF6127CL1
DyLight 350	AF6127D
DyLight 405	AF6127E
DyLight 488	AF6127K
DyLight 550	AF6127L
DyLight 594	AF6127M
DyLight 650	AF6127W
DyLight 680	AF6127Y
DyLight 755	AF6127Z
FITC	AF6127F
HRP	AF6127H
Janelia Fluor 525	AF6127JF525
Janelia Fluor 549	AF6127I
Janelia Fluor 646	AF6127J
Janelia Fluor 669	AF6127JF669
PE	AF6127P
PerCP	AF6127C
mFluor Violet 450 SE	AF6127MFV450
mFluor Violet 500 SE	AF6127MFV500
mFluor Violet 610 SE	AF6127MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6127-SP

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NB600-1317

CD44 Antibody (OX-50) - BSA Free

(1 Review)

(12 Publications)

0.1 ml

NB600-1317

Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1317AF350
Alexa Fluor 405	NB600-1317AF405
Alexa Fluor 488	NB600-1317AF488
Alexa Fluor 532	NB600-1317AF532
Alexa Fluor 594	NB600-1317AF594
Alexa Fluor 647	NB600-1317AF647
Alexa Fluor 700	NB600-1317AF700
Alexa Fluor 750	NB600-1317AF750
Alexa Fluor Plus 405	NB600-1317AFP405
Alexa Fluor Plus 488	NB600-1317AFP488
Alexa Fluor Plus 555	NB600-1317AFP555
Alexa Fluor Plus 594	NB600-1317AFP594
Alexa Fluor Plus 647	NB600-1317AFP647
Alexa Fluor Plus 680	NB600-1317AFP680
Allophycocyanin	NB600-1317APC
Allophycocyanin/Cy7	NB600-1317APCCY7
Biotin	NB600-1317B
CoraFluor 1	NB600-1317CL1
DyLight 350	NB600-1317UV
DyLight 405	NB600-1317V
DyLight 488	NB600-1317G
DyLight 550	NB600-1317R
DyLight 594	NB600-1317DL594
DyLight 650	NB600-1317C
DyLight 680	NB600-1317FR
DyLight 755	NB600-1317IR
FITC	NB600-1317F
HRP	NB600-1317H
Janelia Fluor 525	NB600-1317JF525
Janelia Fluor 549	NB600-1317JF549
Janelia Fluor 646	NB600-1317JF646
Janelia Fluor 669	NB600-1317JF669
PE	NB600-1317PE
PE/Atto594	NB600-1317PEATT594
PE/Cy5.5	NB600-1317PECY55
PE/Cy7	NB600-1317PECY7
PerCP	NB600-1317PCP
mFluor Violet 450 SE	NB600-1317MFV450
mFluor Violet 500 SE	NB600-1317MFV500
mFluor Violet 610 SE	NB600-1317MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1317SS
Azide and BSA Free	NBP2-80649

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NBP2-22530

CD44 Antibody (Hermes-1) - Azide and BSA Free

(8 Publications)

200 μg

NBP2-22530

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Reactivity:

Hu, Mu, Bv, Gt, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Bv, Gt, Rb, Sh

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22530AF350
Alexa Fluor 405	NBP2-22530AF405
Alexa Fluor 488	NBP2-22530AF488
Alexa Fluor 532	NBP2-22530AF532
Alexa Fluor 594	NBP2-22530AF594
Alexa Fluor 647	NBP2-22530AF647
Alexa Fluor 700	NBP2-22530AF700
Alexa Fluor 750	NBP2-22530AF750
Allophycocyanin	NBP2-22530APC
Biotin	NBP2-22530B
CoraFluor 1	NBP2-22530CL1
DyLight 350	NBP2-22530UV
DyLight 405	NBP2-22530V
DyLight 488	NBP2-22530G
DyLight 550	NBP2-22530R-0.2ml
DyLight 594	NBP2-22530DL594
DyLight 650	NBP2-22530C
DyLight 680	NBP2-22530FR
DyLight 755	NBP2-22530IR
FITC	NBP2-22530F
HRP	NBP2-22530H
Janelia Fluor 525	NBP2-22530JF525
Janelia Fluor 549	NBP2-22530JF549
Janelia Fluor 646	NBP2-22530JF646
Janelia Fluor 669	NBP2-22530JF669
PE	NBP2-22530PE
PerCP	NBP2-22530PCP
mFluor Violet 450 SE	NBP2-22530MFV450
mFluor Violet 500 SE	NBP2-22530MFV500
mFluor Violet 610 SE	NBP2-22530MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-22530-50ug

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NBP1-41266

CD44 Antibody (IM7) - BSA Free

(1 Review)

(4 Publications)

0.1 mg

NBP1-41266

Analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.

Reactivity:

Hu, Mu, Ca, Eq, Fe

Species Glossary

Reactivity: Hu, Mu, Ca, Eq, Fe

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-41266AF350
Alexa Fluor 405	NBP1-41266AF405
Alexa Fluor 488	NBP1-41266AF488
Alexa Fluor 532	NBP1-41266AF532
Alexa Fluor 594	NBP1-41266AF594
Alexa Fluor 647	NBP1-41266AF647
Alexa Fluor 700	NBP1-41266AF700
Alexa Fluor 750	NBP1-41266AF750
Alexa Fluor Plus 405	NBP1-41266AFP405
Alexa Fluor Plus 488	NBP1-41266AFP488
Alexa Fluor Plus 555	NBP1-41266AFP555
Alexa Fluor Plus 594	NBP1-41266AFP594
Alexa Fluor Plus 647	NBP1-41266AFP647
Alexa Fluor Plus 680	NBP1-41266AFP680
Allophycocyanin	NBP1-41266APC
Allophycocyanin/Cy7	NBP1-41266APCCY7
Biotin	NBP1-41266B
CoraFluor 1	NBP1-41266CL1
DyLight 350	NBP1-41266UV
DyLight 405	NBP1-41266V
DyLight 488	NBP1-41266G
DyLight 550	NBP1-41266R
DyLight 594	NBP1-41266DL594
DyLight 650	NBP1-41266C
DyLight 680	NBP1-41266FR
DyLight 755	NBP1-41266IR
FITC	NBP1-41266F
HRP	NBP1-41266H
Janelia Fluor 525	NBP1-41266JF525
Janelia Fluor 549	NBP1-41266JF549
Janelia Fluor 646	NBP1-41266JF646
Janelia Fluor 669	NBP1-41266JF669
PE	NBP1-41266PE
PE/Atto594	NBP1-41266PEATT594
PE/Cy5.5	NBP1-41266PECY55
PE/Cy7	NBP1-41266PECY7
PerCP	NBP1-41266PCP
mFluor Violet 450 SE	NBP1-41266MFV450
mFluor Violet 500 SE	NBP1-41266MFV500
mFluor Violet 610 SE	NBP1-41266MFV610

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MAB7045

CD44 Antibody (691534) [Unconjugated] - s Pan Specific

(8 Publications)

100 μg

MAB7045

MAB7045) at 1 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD44 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD44, Monoclonal Antibody (<a class=

MAB7045) at 1 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD44 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD44, Monoclonal Antibody (MAB7045) at 1 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF007). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF007). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_lightbox">

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (<a class=

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD44 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD44 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7045U-100UG
Alexa Fluor 405	FAB7045V-100UG
Alexa Fluor 532	FAB7045X
Alexa Fluor 594	FAB7045T-100UG
Alexa Fluor 647	FAB7045R-100UG
Alexa Fluor 700	FAB7045N-100UG
Alexa Fluor 750	FAB7045S-100UG
Alexa Fluor Plus 405	FAB7045AFP405-100UG
Alexa Fluor Plus 488	FAB7045AFP488-100UG
Alexa Fluor Plus 555	FAB7045AFP555-100UG
Alexa Fluor Plus 594	FAB7045AFP594-100UG
Alexa Fluor Plus 647	FAB7045AFP647-100UG
Alexa Fluor Plus 680	FAB7045AFP680-100UG
Allophycocyanin	FAB7045A
Allophycocyanin/Cy7	FAB7045APCCY7
Biotin	FAB7045B
CoraFluor 1	FAB7045CL1
DyLight 350	FAB7045D
DyLight 405	FAB7045E
DyLight 488	FAB7045K
DyLight 550	FAB7045L
DyLight 594	FAB7045M
DyLight 650	FAB7045W
DyLight 680	FAB7045Y
DyLight 755	FAB7045Z
FITC	FAB7045F
HRP	FAB7045H
Janelia Fluor 525	FAB7045JF525
Janelia Fluor 549	FAB7045I
Janelia Fluor 646	FAB7045J
Janelia Fluor 669	FAB7045JF669
PE/Atto594	FAB7045PEATT594
PE/Cy5.5	FAB7045PECY55
PE/Cy7	FAB7045PECY7
PerCP	FAB7045C
mFluor Violet 450 SE	FAB7045MFV450
mFluor Violet 500 SE	FAB7045MFV500
mFluor Violet 610 SE	FAB7045MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB7045R-100UG
Carrier Free	MAB7045-SP
Carrier Free	FAB7045V-100UG
Carrier Free	FAB7045U-100UG
Carrier Free	FAB7045T-100UG
Carrier Free	FAB7045S-100UG
Carrier Free	FAB7045N-100UG

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NBP1-84578

CD44 Antibody - BSA Free

(1 Review)

(8 Publications)

0.1 ml

NBP1-84578

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines U-251MG and MCF-7 using Anti-CD44 antibody. Corresponding CD44 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-84578-25ul

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BBA11

CD44 Antibody (3G5) [Unconjugated] - v3

(1 Review)

(8 Publications)

200 μg

BBA11

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human CD44 v3 Monoclonal Antibody (Catalog # BBA11) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=

Fluorescent ICC Staining of Non-adherent Cells." alt="CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human CD44 v3 Monoclonal Antibody (Catalog # BBA11) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB003, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_lightbox">

MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (<a class=

MAB003, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5088U-100UG
Alexa Fluor 405	FAB5088V-100UG
Alexa Fluor 488	FAB5088G-100UG
Alexa Fluor 532	BBA11X-100UG
Alexa Fluor 594	FAB5088T-100UG
Alexa Fluor 647	FAB5088R-100UG
Alexa Fluor 700	FAB5088N-100UG
Alexa Fluor 750	FAB5088S-100UG
Alexa Fluor Plus 405	BBA11AFP405-100UG
Alexa Fluor Plus 488	BBA11AFP488-100UG
Alexa Fluor Plus 555	BBA11AFP555-100UG
Alexa Fluor Plus 594	BBA11AFP594-100UG
Alexa Fluor Plus 647	BBA11AFP647-100UG
Alexa Fluor Plus 680	BBA11AFP680-100UG
Biotin	BBA11B
CoraFluor 1	BBA11CL1
DyLight 350	BBA11D
DyLight 405	BBA11E
DyLight 488	BBA11K
DyLight 550	BBA11L
DyLight 594	BBA11M
DyLight 650	BBA11W
DyLight 680	BBA11Y
DyLight 755	BBA11Z
FITC	BBA11F
HRP	BBA11H
Janelia Fluor 525	BBA11JF525
Janelia Fluor 549	BBA11I
Janelia Fluor 646	BBA11J
Janelia Fluor 669	BBA11JF669
PerCP	BBA11C
mFluor Violet 450 SE	BBA11MFV450
mFluor Violet 500 SE	BBA11MFV500
mFluor Violet 610 SE	BBA11MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB5088G-100UG
Carrier Free	FAB5088U-100UG
Carrier Free	FAB5088R-100UG
Carrier Free	FAB5088S-100UG
Carrier Free	FAB5088V-100UG
Carrier Free	FAB5088T-100UG
Carrier Free	FAB5088N-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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