| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89692-25ul | |||
CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="SMARCA5/SNF2H was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human SMARCA5/SNF2H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6825) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6825-SP | |||
![Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - SNF2H Antibody [NB100-55310] - Detection of Human SNF2h/ISWI on HeLa whole cell lysate using NB100-55310. SNF2h/ISWI was also immunoprecipitated by rabbit anti-SNF2h/ISWI antibodies BL4495 and NB100-55311.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NB100-55310-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - SNF2H Antibody [NB100-55310] - Detection of Human SNF2h/ISWI on HeLa whole cell lysate using NB100-55310. SNF2h/ISWI was also immunoprecipitated by rabbit anti-SNF2h/ISWI antibodies BL4495 and NB100-55311.")
![<b>Independent Antibodies Validation. </b>Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - WB analysis of SNF2H in Mouse Stem Cell lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NB100-55310-img0003.jpg)
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0005.jpg "Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of Hep G2 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16216_rabbit-smarca5-snf2h-mab-6h8i7-5220251242527.jpg)
![Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of C6 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16216_rabbit-smarca5-snf2h-mab-6h8i7-52202511365146.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00008467-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunohistochemistry-Paraffin-H00008467-M02-img0005.jpg)
![Sandwich ELISA: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Detection limit for recombinant GST tagged SMARCA5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Sandwich-ELISA-H00008467-M02-img0006.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP3-12952-img0001.jpg "Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.")
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP3-12952] - SMARCA5/SNF2H antibody detects SMARCA5/SNF2H protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunohistochemistry-Paraffin-NBP3-12952-img0002.jpg)
![Western Blot: SMARCA5/SNF2H Antibody (S05-9D5) [NBP3-15093] - Western blot detection of SMARCA5/SNF2H in K562, C6, 3T3, Hela cell lysates using SMARCA5/SNF2H Rabbit mAb (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 122kDa.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-S05-9D5-Western-Blot-NBP3-15093-img0001.jpg "Western Blot: SMARCA5/SNF2H Antibody (S05-9D5) [NBP3-15093] - Western blot detection of SMARCA5/SNF2H in K562, C6, 3T3, Hela cell lysates using SMARCA5/SNF2H Rabbit mAb (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 122kDa.")
![Western Blot: SNF2H Overexpression Lysate (Adult Normal) [NBL1-16228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNF2H.](http://images.novusbio.com/fullsize/SNF2H-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16228-img0002.jpg "Western Blot: SNF2H Overexpression Lysate (Adult Normal) [NBL1-16228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNF2H.")
![Chromatin Immunoprecipitation: SMARCA5/SNF2H Antibody [NBP3-10883] - Chromatin Immunoprecipitation (ChIP) using NBP3-10883 and HCT116 Cells](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Chromatin-Immunoprecipitation-NBP3-10883-img0001.jpg "Chromatin Immunoprecipitation: SMARCA5/SNF2H Antibody [NBP3-10883] - Chromatin Immunoprecipitation (ChIP) using NBP3-10883 and HCT116 Cells")
![Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunoprecipitation-NB100-55311-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.")
