210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (
HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (
MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (
HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (
MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (
210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (
HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (
MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (
F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox">

MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (
F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />