SOX10: Novus Biologicals

AF2864

SOX10 Antibody [Unconjugated]

(2 Reviews)

100 μg

AF2864

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Adult mouse (P6W) brain perfused with 4% paraformaldehyde/PBS, 30 um free floating section. Block with 1% BSA/0.3% Triton X-100/PBS for 30min, primary antibody (1:1000, 0.2ug/ml), secondary antibody (anti-goat Alexa Fluor 488). Antigen retrieval treatment was not needed. Image from a verified customer review.

PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Adult dynamin 2-depleted Schwann cells are replaced by dynamin 2-positive Schwann cells in remyelinated nerves.(A) Immunostainings for DNM2, SOX10 (SCs) and YFP (reporter-recombined SCs) on SN cross-sections of control* (MpzCreERT2:Rosa26-stoploxP/loxP-YFP) and P0ERT2-Dnm2KO* (MpzCreERT2:Dnm2loxP/loxP: Rosa26-stoploxP/loxP-YFP) mice at 4 wpT and 14 wpT. White arrows: YFP-recombined SCs that express DNM2 (in controls at 4 wpt and 14 wpt); black arrowheads: YFP-recombined SCs lacking DNM2 (in mutants at 4 wpt); white arrowheads: Non-recombined SCs expressing DNM2 (in controls and mutants at 4 wpt and 14 wpt). Scale bar = 25 μm for entire panel. (B) Quantification related to (A). Percentage of YFP+ cells among SOX10+ SCs/SN cross-sections at 4 wpT, 6 wpT, and 14 wpT. N = 3 mice/genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (C) Western blot analysis of cleaved caspase 3 (cC3) in SN lysates of control* and P0ERT2-Dnm2KO* mice at 4 wpT, 6 wpT and 14 wpT. Full-length blots in Supplementary file 1F. (D) Quantification referring to (C). Control average was set to 1. N = 3 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. Results in graphs represent means ±s.e.m.; ***p<0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Schwann cells lacking dynamin 2 show impaired cell cycle progression, reduced mitosis rate, and cytokinesis defects.(A) Schematic representation of the cell cycle phases marked by Ki-67 and EdU. (B) EdU-labeling, combined with immunostainings for Ki-67 and SOX10 on control and P0-Dnm2KO SN cross-sections at P5. Arrows: EdU+ Ki-67+ SOX10+ SCs, arrowheads: Ki-67+ SOX10+ SCs. Scale bar = 25 μm for entire panel. (C) Quantification of (B). Percentage of EdU+ SCs/SN cross-sections. N = 5 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (D) Quantification of (B). Percentage of Ki-67+ SCs/SN cross-sections at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (E) Quantification of (B). Percentage of EdU+ among Ki-67+ SCs/SN cross-section at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (F) Quantification of mitotic events in cultured mouse SCs isolated from MpzCre:Rosa26-stoploxP/loxP-YFP (control*) and MpzCre:Dnm2loxP/loxP:Rosa26-stoploxP/loxP-YFP (P0-Dnm2KO*) SNs at P1, monitored by time-lapse microscopy for 24 hr. Each data point represents one individual animal (at least 42 cells/animal analyzed). N = 5 controls and seven mutant mice, two-tailed unpaired Student´s t-test. (G) Quantification of mitosis duration (minutes) of SCs of control* and P0-Dnm2KO* mice, monitored by time-lapse microscopy for 24 hr. Each data point represents one cell derived from a total of 5 control and seven mutant mice. Cells derived from each animal were isolated and analyzed separately, but pooled in one graph; two-tailed unpaired Student´s t-test. (H) Exemplary picture of a multinucleated Dnm2KO SCs after 48 hr in culture. Scale bar = 25 μm. (I) Quantification of multinucleated cells in control and Dnm2KO SCs after 24 hr, 48 hr and 76 hr in culture. N = 6 mice/genotype for 48 hr; N = 6 control* and n = 5 P0-Dnm2KO* mice for 24 hr and 72 hr, two-Way ANOVA with Sidak’s multiple comparisons test. (J) Exemplary time-lapse images of

Filamentous actin is increased in Nedd4 knockdown&Nedd4−/− NCCs. e Representative images of migrating trunk NCCs within somites (S) 8–10 in control&Wnt1-Cre; Nedd4fl/fl E9.5 embryos. Whole embryos with 20–23 somites stained with anti-SOX10 (green)&anti-CD31 antibodies (red) to recognise NCCs&blood vessels. Dashed line marks the ventral limit of the dorsal aorta (DA) which was used to normalise migration distance of pioneering NCCs (arrowhead). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35440627), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mice lacking dynamin 2 in Schwann cells display characteristics of inflammation and Schwann cell death. (E) (Left) Immunostainings of controls and P0-Dnm2KO SN cross-sections for cleaved-caspase 3 (cC3) in combination with the SC marker SOX10 at P5. (Right) Percentage of cC3+ SOX10+ SCs at P5 and P14. N = 5 mice/time point and genotype, two-tailed unpaired Student’s t-test. Scale bar = 25 μm for entire panel. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2864U-100UG
Alexa Fluor 405	AF2864V-100UG
Alexa Fluor 488	AF2864G-100UG
Alexa Fluor 532	AF2864X-100UG
Alexa Fluor 594	AF2864T-100UG
Alexa Fluor 647	AF2864R-100UG
Alexa Fluor 700	AF2864N-100UG
Alexa Fluor 750	AF2864S-100UG
Alexa Fluor Plus 405	AF2864AFP405-100UG
Alexa Fluor Plus 488	AF2864AFP488-100UG
Alexa Fluor Plus 555	AF2864AFP555-100UG
Alexa Fluor Plus 594	AF2864AFP594-100UG
Alexa Fluor Plus 647	AF2864AFP647-100UG
Alexa Fluor Plus 680	AF2864AFP680-100UG
Allophycocyanin	AF2864A
Biotin	BAF2864
CoraFluor 1	AF2864CL1
DyLight 350	AF2864D
DyLight 405	AF2864E
DyLight 488	AF2864K
DyLight 550	AF2864L
DyLight 594	AF2864M
DyLight 650	AF2864W
DyLight 680	AF2864Y
DyLight 755	AF2864Z
FITC	AF2864F
HRP	AF2864H
Janelia Fluor 525	AF2864JF525
Janelia Fluor 549	AF2864I
Janelia Fluor 646	AF2864J
Janelia Fluor 669	AF2864JF669
PE	AF2864P
PerCP	AF2864C
mFluor Violet 450 SE	AF2864MFV450
mFluor Violet 500 SE	AF2864MFV500
mFluor Violet 610 SE	AF2864MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2864-SP

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MAB2864

SOX10 Antibody (20B7) [Unconjugated]

(22 Publications)

100 μg

MAB2864

NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Identification of neural crest-derived DPCs. ((a)–(c)) Immunofluorescent cytochemical staining revealed expression of NCSCs-specific markers in DPCs. Primary DPCs were double positive for P75 and Sox10 (a), P75 and Nestin (b), and were positive for Sox9 (c). (d) RT-PCR analysis confirmed the expression of dermal papillae markers ALP and Sox2, and the NCSCs markers Nestin, P75, Sox9, Twist1, and AP2 alpha in DPCs, as well as BMSCs. Scale bar = 20 μm ((a)–(c)). DPCs-P#: DPCs at passage #; BMSCs-P#: BMSCs at passage #. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25045659), licensed under a CC-BY license. Not internally tested by R&D Systems.

JCV T-antigen is expressed in neural crest cells.Fluorescent images of bone marrow cells isolated from JCV T-antigen transgenic mice cultured in neural conditions show that these cells are positive for p75 (A,B), nestin (D,E) and SOX-10 (G,H), which are all markers of the neural crest. JCV T-antigen (J,K) was detected in the nucleus of p75 positive cells. Bone marrow cells cultured in mesenchymal media were negative for p75, nestin and SOX-10, and did not express JCV T-antigen (C, F, I, L). Cell nuclei were visualized with DAPI. (Scale bar: 100 µm). (M) Diagram illustrating expression of JCV T-antigen in bone marrow cells. T-antigen expression was associated with neural crest lineage cells of the bone marrow cultured in serum-free neural stem cell media supplemented with bFGF and EFG (left panel), while mesenchymal cells maintained in alpha -MEM in the presence of 20% serum were negative for T-antigen (right panel). (N) FACS analysis of neural crest cells and MSCs demonstrates positivity for JCV T-antigen in 99% of neural crest cells and lack of JCV T-antigen expression in MSCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23805194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2864U-100UG
Alexa Fluor 405	FAB2864V-100UG
Alexa Fluor 488	FAB2864G-100UG
Alexa Fluor 532	FAB2864X-100UG
Alexa Fluor 594	FAB2864T-100UG
Alexa Fluor 647	FAB2864R-100UG
Alexa Fluor 700	FAB2864N-100UG
Alexa Fluor 750	FAB2864S-100UG
Alexa Fluor Plus 405	FAB2864AFP405-100UG
Alexa Fluor Plus 488	FAB2864AFP488-100UG
Alexa Fluor Plus 555	FAB2864AFP555-100UG
Alexa Fluor Plus 594	FAB2864AFP594-100UG
Alexa Fluor Plus 647	FAB2864AFP647-100UG
Alexa Fluor Plus 680	FAB2864AFP680-100UG
Allophycocyanin	FAB2864A
Biotin	FAB2864B
CoraFluor 1	FAB2864CL1
DyLight 350	FAB2864D
DyLight 405	FAB2864E
DyLight 488	FAB2864K
DyLight 550	FAB2864L
DyLight 594	FAB2864M
DyLight 650	FAB2864W
DyLight 680	FAB2864Y
DyLight 755	FAB2864Z
FITC	FAB2864F
HRP	FAB2864H
Janelia Fluor 525	FAB2864JF525
Janelia Fluor 549	FAB2864I
Janelia Fluor 646	FAB2864J
Janelia Fluor 669	FAB2864JF669
NL557	NL2864R
PE	FAB2864P
PerCP	FAB2864C
mFluor Violet 450 SE	FAB2864MFV450
mFluor Violet 500 SE	FAB2864MFV500
mFluor Violet 610 SE	FAB2864MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	NL2864R
Carrier Free	MAB2864-SP

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NBP2-59620

SOX10 Antibody (SOX10/991)

(3 Publications)

100 μg

NBP2-59620

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) [NBP2-59620] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).

Western Blot: SOX10 Antibody (SOX10/991) [NBP2-59620] - analysis of SOX10 (A) Recombinant protein (B) A375 Cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/991).

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) [NBP2-59620] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).

Simple Western: SOX10 Antibody (SOX10/991) [NBP2-59620] - Analysis of SOX10 antibody on mouse hippocampus sample. Primary antibody dilution: 1:50.1 ug/uL protein. Image from verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-59621AF350
Alexa Fluor 405	NBP2-59621AF405
Alexa Fluor 488	NBP2-59621AF488
Alexa Fluor 532	NBP2-59621AF532
Alexa Fluor 594	NBP2-59621AF594
Alexa Fluor 647	NBP2-59621AF647
Alexa Fluor 700	NBP2-59621AF700
Alexa Fluor 750	NBP2-59621AF750
Alexa Fluor Plus 405	NBP2-59621AFP405
Alexa Fluor Plus 488	NBP2-59621AFP488
Alexa Fluor Plus 555	NBP2-59621AFP555
Alexa Fluor Plus 594	NBP2-59621AFP594
Alexa Fluor Plus 647	NBP2-59621AFP647
Alexa Fluor Plus 680	NBP2-59621AFP680
Allophycocyanin	NBP2-59621APC
Biotin	NBP2-59621B
CoraFluor 1	NBP2-59621CL1
DyLight 350	NBP2-59621UV
DyLight 405	NBP2-59621V
DyLight 488	NBP2-59621G
DyLight 550	NBP2-59621R
DyLight 594	NBP2-59621DL594
DyLight 650	NBP2-59621C
DyLight 680	NBP2-59621FR
DyLight 755	NBP2-59621IR
FITC	NBP2-59621F
HRP	NBP2-59621H
Janelia Fluor 525	NBP2-59621JF525
Janelia Fluor 549	NBP2-59621JF549
Janelia Fluor 646	NBP2-59621JF646
Janelia Fluor 669	NBP2-59621JF669
PE	NBP2-59621PE
PerCP	NBP2-59621PCP
mFluor Violet 450 SE	NBP2-59621MFV450
mFluor Violet 500 SE	NBP2-59621MFV500
mFluor Violet 610 SE	NBP2-59621MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-48436
Azide and BSA Free	NBP2-59621

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NBP2-59050

SOX10 Antibody (CL4455) - BSA Free

(1 Review)

100 ul

NBP2-59050

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.

Staining of mouse embryo E11 shows strong positivity in the developing trigeminal ganglion.

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).

Staining of SK-MEL-30 cells, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44116AF350
Alexa Fluor 405	NBP3-44116AF405
Alexa Fluor 488	NBP3-44116AF488
Alexa Fluor 532	NBP3-44116AF532
Alexa Fluor 594	NBP3-44116AF594
Alexa Fluor 647	NBP3-44116AF647
Alexa Fluor 700	NBP3-44116AF700
Alexa Fluor 750	NBP3-44116AF750
Alexa Fluor Plus 405	NBP3-44116AFP405
Alexa Fluor Plus 488	NBP3-44116AFP488
Alexa Fluor Plus 555	NBP3-44116AFP555
Alexa Fluor Plus 594	NBP3-44116AFP594
Alexa Fluor Plus 647	NBP3-44116AFP647
Alexa Fluor Plus 680	NBP3-44116AFP680
Allophycocyanin	NBP3-44116APC
Biotin	NBP3-44116B
CoraFluor 1	NBP3-44116CL1
DyLight 350	NBP3-44116UV
DyLight 405	NBP3-44116V
DyLight 488	NBP3-44116G
DyLight 550	NBP3-44116R
DyLight 594	NBP3-44116DL594
DyLight 650	NBP3-44116C
DyLight 680	NBP3-44116FR
DyLight 755	NBP3-44116IR
FITC	NBP3-44116F
HRP	NBP3-44116H
Janelia Fluor 525	NBP3-44116JF525
Janelia Fluor 549	NBP3-44116JF549
Janelia Fluor 646	NBP3-44116JF646
Janelia Fluor 669	NBP3-44116JF669
PE	NBP3-44116PE
PerCP	NBP3-44116PCP
mFluor Violet 450 SE	NBP3-44116MFV450
mFluor Violet 500 SE	NBP3-44116MFV500
mFluor Violet 610 SE	NBP3-44116MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44116
BSA Free	NBP2-59050-25ul

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NBP2-57023

SOX10 Antibody - BSA Free

100 ul

NBP2-57023

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP2-57023] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP2-57023] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP2-57023] - Staining of human liver shows no positivity in hepatocytes as expected.

ChIP-Exo-Seq composite graph for Anti-SOX10 (NBP2-57023) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57023-25ul

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NBP2-67812

SOX10 Antibody (SD204-04)

100 ul

NBP2-67812

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded mouse brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.

Western blot analysis on different lysates with NBP2-67812 at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: B16-F1 cell lysate Lane 3: A375 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 50 kDa Observed band size: 60/75 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67812 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on different lysates with NBP2-67812 at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 60/75 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67812 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-59622

SOX10 Antibody (SOX10/1074)

100 μg

NBP2-59622

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).

Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622] - analysis of SOX10 (A) Recombinant protein; (B) A375 Cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/1074)

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).

Simple Western: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Analysis of SOX10 antibody on mouse hippocampus sample. Primary antibody dilution: 1:50. 1 ug/uL protein. Image from verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-59623AF350
Alexa Fluor 405	NBP2-59623AF405
Alexa Fluor 488	NBP2-59623AF488
Alexa Fluor 532	NBP2-59623AF532
Alexa Fluor 594	NBP2-59623AF594
Alexa Fluor 647	NBP2-59623AF647
Alexa Fluor 700	NBP2-59623AF700
Alexa Fluor 750	NBP2-59623AF750
Alexa Fluor Plus 405	NBP2-59623AFP405
Alexa Fluor Plus 488	NBP2-59623AFP488
Alexa Fluor Plus 555	NBP2-59623AFP555
Alexa Fluor Plus 594	NBP2-59623AFP594
Alexa Fluor Plus 647	NBP2-59623AFP647
Alexa Fluor Plus 680	NBP2-59623AFP680
Allophycocyanin	NBP2-59623APC
Biotin	NBP2-59623B
CoraFluor 1	NBP2-59623CL1
DyLight 350	NBP2-59623UV
DyLight 405	NBP2-59623V
DyLight 488	NBP2-59623G
DyLight 550	NBP2-59623R
DyLight 594	NBP2-59623DL594
DyLight 650	NBP2-59623C
DyLight 680	NBP2-59623FR
DyLight 755	NBP2-59623IR
FITC	NBP2-59623F
HRP	NBP2-59623H
Janelia Fluor 525	NBP2-59623JF525
Janelia Fluor 549	NBP2-59623JF549
Janelia Fluor 646	NBP2-59623JF646
Janelia Fluor 669	NBP2-59623JF669
PE	NBP2-59623PE
mFluor Violet 450 SE	NBP2-59623MFV450
mFluor Violet 500 SE	NBP2-59623MFV500
mFluor Violet 610 SE	NBP2-59623MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-59623

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NBP1-68983

SOX10 Antibody - BSA Free

(3 Publications)

100 ul

NBP1-68983

Western Blot: SOX10 Antibody [NBP1-68983] - HepG2, 0.25ug/ml.

Western Blot: SOX10 Antibody [NBP1-68983] - SOX10 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.

Western Blot: SOX10 Antibody [NBP1-68983] - Sample Tissue: Jurkat cell lysates Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/ml Peptide Concentration: 4.0ug/ml Lysate Quantity: 25ug/lane Gel Concentration:

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP1-68983] - Human Kidney Tissue. Arrow indicates epithelial cells of renal tubule with positive label.

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP1-68983] - Human Skin Tissue with epidermal cells positively labeled.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-44475

SOX10 Antibody (SPM607)

(1 Publication)

0.1 mg

NBP2-44475

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47708AF350
Alexa Fluor 405	NBP2-47708AF405
Alexa Fluor 488	NBP2-47708AF488
Alexa Fluor 532	NBP2-47708AF532
Alexa Fluor 594	NBP2-47708AF594
Alexa Fluor 647	NBP2-47708AF647
Alexa Fluor 700	NBP2-47708AF700
Alexa Fluor 750	NBP2-47708AF750
Alexa Fluor Plus 405	NBP2-47708AFP405
Alexa Fluor Plus 488	NBP2-47708AFP488
Alexa Fluor Plus 555	NBP2-47708AFP555
Alexa Fluor Plus 594	NBP2-47708AFP594
Alexa Fluor Plus 647	NBP2-47708AFP647
Alexa Fluor Plus 680	NBP2-47708AFP680
Allophycocyanin	NBP2-47708APC
Biotin	NBP2-47708B
CoraFluor 1	NBP2-47708CL1
DyLight 350	NBP2-47708UV
DyLight 405	NBP2-47708V
DyLight 488	NBP2-47708G
DyLight 550	NBP2-47708R
DyLight 594	NBP2-47708DL594
DyLight 650	NBP2-47708C
DyLight 680	NBP2-47708FR
DyLight 755	NBP2-47708IR
FITC	NBP2-47708F
HRP	NBP2-47708H
Janelia Fluor 525	NBP2-47708JF525
Janelia Fluor 549	NBP2-47708JF549
Janelia Fluor 646	NBP2-47708JF646
Janelia Fluor 669	NBP2-47708JF669
PE	NBP2-47708PE
PerCP	NBP2-47708PCP
mFluor Violet 450 SE	NBP2-47708MFV450
mFluor Violet 500 SE	NBP2-47708MFV500
mFluor Violet 610 SE	NBP2-47708MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47708-0.1mg
IHC-Prediluted	NBP2-48444

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NBP3-32970

SOX10 Antibody (PDH0-03)

100 ul

NBP3-32970

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-03) [NBP3-32970] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: SOX10 Antibody (PDH0-03) [NBP3-32970] - Flow cytometric analysis of A375 cells labeling SOX10.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32970, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western blot analysis on different lysates with NBP3-32970 at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: B16-F1 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 60-75 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32970 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-32971

SOX10 Antibody (PDH0-04)

100 ul

NBP3-32971

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-04) [NBP3-32971] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: SOX10 Antibody (PDH0-04) [NBP3-32971] - Flow cytometric analysis of A375 cells labeling SOX10.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32971, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western blot analysis on different lysates with NBP3-32971 at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: B16-F1 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 60-75 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32971 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-32969

SOX10 Antibody (PDH0-05)

100 ul

NBP3-32969

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-05) [NBP3-32969] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: SOX10 Antibody (PDH0-05) [NBP3-32969] - Flow cytometric analysis of A375 cells labeling SOX10.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32969, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western blot analysis on different lysates with NBP3-32969 at 1/2,000 dilution. Lane 1: A375 cell lysate Lane 2: B16-F1 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 60-75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32969 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

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SOX10 Antibody (PDH0-04)

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