Sortilin: Novus Biologicals

AF2934

Sortilin Antibody [Unconjugated]

(8 Reviews)

100 μg

AF2934

HAF017). A specific band was detected for Sortilin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Sortilin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Sortilin was detected in perfusion fixed frozen sections of mouse brain (rostral ventral medulla) using Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse brain (cortex) tissue , loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 122 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Primary cultured Schwann cells express sortilin. (A) Double-labeled immunofluorescence microscopy of primary cultured Schwann cells show sortilin (green) in wild-type (WT) Schwann cells (red; identified by the Schwann cell marker s100 beta ), but not in cells derived from Sort1–/– rat sciatic nerves. Nuclei are labeled with Hoechst (blue). Scale bar 50 μm. (B) Immunoblot analysis of sortilin in lysates of WT or Sort1–/– primary Schwann cells shows sortilin expression in WT. (C) The viability of Sort1–/– Schwann cells is like WT Schwann cells with (95.89 ± 1.55% vs. 94.30 ± 1.77%, p > 0.99) or without NT-3 stimulation (93.8 ± 1.52% vs. 93.29 ± 1.89%, p > 0.99). Viable cells were identified in Schwann cell cultures 24 h after stimulation with or without NT-3 by calcein (green) fluorescence, while dead cells were identified by ethidium homodimer-1 (red) fluorescence. Scale bar 100 μm. Viability reflects mean% calcein-positive cells ± SEM, n = 4. (D) Schwann cell proliferation (percentage of Ki-67% cells) was not significantly different in cultures of Sort1–/– Schwann cells compared to WT Schwann cell cultures with (4.82 ± 1.54% vs. 1.05 ± 0.25%, p = 0.4381) or without NT-3 stimulation (4.09 ± 1.98% vs. 1.37 ± 0.56%, p > 0.99). The proliferation marker, Ki-67 (green), was identified by immunofluorescence in Schwann cell cultures 48 h after stimulation with or without NT-3. Nuclei are labeled with Hoechst (blue). Scale bar 100 μm. Proliferation rates reflect% Ki-67 positive cells ± S.E.M., n = 3. Statistical significance was analyzed using two-way ANOVA with Bonferroni’s multiple comparisons post hoc test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35634462), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neurotrophin signaling is decreased in Sort1–/– Schwann cells. (A) pAkt, pMAPK, pRSK, and pCREB were quantified by densitometry of immunoblots from lysates of NT-3 or BDNF stimulated wild-type (WT) or Sort1–/– primary cultures of Schwann cells and normalized against beta -actin. pMAPK, pRSK, and pCREB levels are significantly lower in Sort1–/– Schwann cells compared to WT Schwann cells. Data represent the mean ± SEM for n = 3. (B) The relative wound healing closure time was similar in Sort1–/– Schwann cells relative to WT Schwann cells with or without 10% FBS (positive control) or NT-3. Furthermore, the relative wound healing closure time indicates that NT-3 does not increase the relative wound healing closure time significantly in WT Schwann cells compared to unstimulated controls; 10% FBS significantly increased the relative wound healing closure time at 27–39 h (*p < 0.05). (C)Sort1–/– Schwann cells showed similar myelin formation relative to WT Schwann cells. Co-cultures of DRG neurons and Schwann cells were fixed 11 days after myelin induction with or without NT-3 stimulation. The myelin segments were identified with an antibody against myelin basic protein (MBP; green) and the neurites were identified with antibodies against neurofilament (red). Hoechst identifies nuclei (blue). Statistical significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons post hoc test. Data represents mean ± SEM, n = 3 (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35634462), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2934U-100UG
Alexa Fluor 405	AF2934V-100UG
Alexa Fluor 488	AF2934G-100UG
Alexa Fluor 532	AF2934X-100UG
Alexa Fluor 594	AF2934T-100UG
Alexa Fluor 647	AF2934R-100UG
Alexa Fluor 700	AF2934N-100UG
Alexa Fluor 750	AF2934S-100UG
Alexa Fluor Plus 405	AF2934AFP405-100UG
Alexa Fluor Plus 488	AF2934AFP488-100UG
Alexa Fluor Plus 555	AF2934AFP555-100UG
Alexa Fluor Plus 594	AF2934AFP594-100UG
Alexa Fluor Plus 647	AF2934AFP647-100UG
Alexa Fluor Plus 680	AF2934AFP680-100UG
Allophycocyanin	AF2934A
Biotin	BAF2934
CoraFluor 1	AF2934CL1
DyLight 350	AF2934D
DyLight 405	AF2934E
DyLight 488	AF2934K
DyLight 550	AF2934L
DyLight 594	AF2934M
DyLight 650	AF2934W
DyLight 680	AF2934Y
DyLight 755	AF2934Z
FITC	AF2934F
HRP	AF2934H
Janelia Fluor 525	AF2934JF525
Janelia Fluor 549	AF2934I
Janelia Fluor 646	AF2934J
Janelia Fluor 669	AF2934JF669
PE	AF2934P
PerCP	AF2934C
mFluor Violet 450 SE	AF2934MFV450
mFluor Violet 500 SE	AF2934MFV500
mFluor Violet 610 SE	AF2934MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2934-SP

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AF3154

Sortilin Antibody [Unconjugated]

(1 Review)

(9 Publications)

100 μg

AF3154

HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood monocytes were stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Sortilin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 82 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3154U-100UG
Alexa Fluor 405	AF3154V-100UG
Alexa Fluor 488	AF3154G-100UG
Alexa Fluor 532	AF3154X-100UG
Alexa Fluor 594	AF3154T-100UG
Alexa Fluor 647	AF3154R-100UG
Alexa Fluor 700	AF3154N-100UG
Alexa Fluor 750	AF3154S-100UG
Alexa Fluor Plus 405	AF3154AFP405-100UG
Alexa Fluor Plus 488	AF3154AFP488-100UG
Alexa Fluor Plus 555	AF3154AFP555-100UG
Alexa Fluor Plus 594	AF3154AFP594-100UG
Alexa Fluor Plus 647	AF3154AFP647-100UG
Alexa Fluor Plus 680	AF3154AFP680-100UG
Allophycocyanin	AF3154A
Biotin	BAF3154
CoraFluor 1	AF3154CL1
DyLight 350	AF3154D
DyLight 405	AF3154E
DyLight 488	AF3154K
DyLight 550	AF3154L
DyLight 594	AF3154M
DyLight 650	AF3154W
DyLight 680	AF3154Y
DyLight 755	AF3154Z
FITC	AF3154F
HRP	AF3154H
Janelia Fluor 525	AF3154JF525
Janelia Fluor 549	AF3154I
Janelia Fluor 646	AF3154J
Janelia Fluor 669	AF3154JF669
PE	AF3154P
PerCP	AF3154C
mFluor Violet 450 SE	AF3154MFV450
mFluor Violet 500 SE	AF3154MFV500
mFluor Violet 610 SE	AF3154MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3154-SP

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MAB3154

Sortilin Antibody (334708) [Unconjugated]

(3 Publications)

100 μg

MAB3154

Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Sortilin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3154U-100UG
Alexa Fluor 405	FAB3154V-100UG
Alexa Fluor 488	FAB3154G-100UG
Alexa Fluor 532	FAB3154X-100UG
Alexa Fluor 594	FAB3154T-100UG
Alexa Fluor 647	FAB3154R-100UG
Alexa Fluor 700	FAB3154N-100UG
Alexa Fluor 750	FAB3154S-100UG
Alexa Fluor Plus 405	FAB3154AFP405-100UG
Alexa Fluor Plus 488	FAB3154AFP488-100UG
Alexa Fluor Plus 555	FAB3154AFP555-100UG
Alexa Fluor Plus 594	FAB3154AFP594-100UG
Alexa Fluor Plus 647	FAB3154AFP647-100UG
Alexa Fluor Plus 680	FAB3154AFP680-100UG
Allophycocyanin	FAB3154A
Biotin	FAB3154B
CoraFluor 1	FAB3154CL1
DyLight 350	FAB3154D
DyLight 405	FAB3154E
DyLight 488	FAB3154K
DyLight 550	FAB3154L
DyLight 594	FAB3154M
DyLight 650	FAB3154W
DyLight 680	FAB3154Y
DyLight 755	FAB3154Z
FITC	FAB3154F
HRP	FAB3154H
Janelia Fluor 525	FAB3154JF525
Janelia Fluor 549	FAB3154I
Janelia Fluor 646	FAB3154J
Janelia Fluor 669	FAB3154JF669
PE	FAB3154P
PerCP	FAB3154C
mFluor Violet 450 SE	FAB3154MFV450
mFluor Violet 500 SE	FAB3154MFV500
mFluor Violet 610 SE	FAB3154MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3154-SP

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NBP2-76498

Sortilin Antibody (CL6526) - BSA Free

100 ul

NBP2-76498

Immunocytochemistry/Immunofluorescence: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of RH-30 cells using the Anti-SORT1 monoclonal antibody, showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: Sortilin Antibody (CL6526) [NBP2-76498] - Analysis in human cell line SK-MEL-30.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human lymph node shows only weak positivity in a subset of lymphoid cells, as expected.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of mouse hippocampus shows strong cytoplasmic positivity in both pyramidal neurons and granular cells.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44414AF350
Alexa Fluor 405	NBP3-44414AF405
Alexa Fluor 488	NBP3-44414AF488
Alexa Fluor 532	NBP3-44414AF532
Alexa Fluor 594	NBP3-44414AF594
Alexa Fluor 647	NBP3-44414AF647
Alexa Fluor 700	NBP3-44414AF700
Alexa Fluor 750	NBP3-44414AF750
Alexa Fluor Plus 405	NBP3-44414AFP405
Alexa Fluor Plus 488	NBP3-44414AFP488
Alexa Fluor Plus 555	NBP3-44414AFP555
Alexa Fluor Plus 594	NBP3-44414AFP594
Alexa Fluor Plus 647	NBP3-44414AFP647
Alexa Fluor Plus 680	NBP3-44414AFP680
Allophycocyanin	NBP3-44414APC
Biotin	NBP3-44414B
CoraFluor 1	NBP3-44414CL1
DyLight 350	NBP3-44414UV
DyLight 405	NBP3-44414V
DyLight 488	NBP3-44414G
DyLight 550	NBP3-44414R
DyLight 594	NBP3-44414DL594
DyLight 650	NBP3-44414C
DyLight 680	NBP3-44414FR
DyLight 755	NBP3-44414IR
FITC	NBP3-44414F
HRP	NBP3-44414H
Janelia Fluor 525	NBP3-44414JF525
Janelia Fluor 549	NBP3-44414JF549
Janelia Fluor 646	NBP3-44414JF646
Janelia Fluor 669	NBP3-44414JF669
PE	NBP3-44414PE
PerCP	NBP3-44414PCP
mFluor Violet 450 SE	NBP3-44414MFV450
mFluor Violet 500 SE	NBP3-44414MFV500
mFluor Violet 610 SE	NBP3-44414MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44414
BSA Free	NBP2-76498-25ul

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NBP3-52100

Sortilin Antibody (CL6526)

100 ul

NBP3-52100

Staining of human lymph node shows only weak positivity in a subset of lymphoid cells, as expected.

Staining of RH-30 cells showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Staining of mouse hippocampus shows strong cytoplasmic positivity in both pyramidal neurons and granular cells.

Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.

Analysis in human cerebral cortex and lymph node tissues. Corresponding Sortilin RNA-seq data are presented for the same tissues.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-76501

Sortilin Antibody (CL6528) - BSA Free

100 ul

NBP2-76501

Western Blot: Sortilin Antibody (CL6528) [NBP2-76501] - Analysis in human cell line SK-MEL-30.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Analysis in human cerebral cortex and tonsil tissues. Corresponding SORT1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44415AF350
Alexa Fluor 405	NBP3-44415AF405
Alexa Fluor 488	NBP3-44415AF488
Alexa Fluor 532	NBP3-44415AF532
Alexa Fluor 594	NBP3-44415AF594
Alexa Fluor 647	NBP3-44415AF647
Alexa Fluor 700	NBP3-44415AF700
Alexa Fluor 750	NBP3-44415AF750
Alexa Fluor Plus 405	NBP3-44415AFP405
Alexa Fluor Plus 488	NBP3-44415AFP488
Alexa Fluor Plus 555	NBP3-44415AFP555
Alexa Fluor Plus 594	NBP3-44415AFP594
Alexa Fluor Plus 647	NBP3-44415AFP647
Alexa Fluor Plus 680	NBP3-44415AFP680
Allophycocyanin	NBP3-44415APC
Biotin	NBP3-44415B
CoraFluor 1	NBP3-44415CL1
DyLight 350	NBP3-44415UV
DyLight 405	NBP3-44415V
DyLight 488	NBP3-44415G
DyLight 550	NBP3-44415R
DyLight 594	NBP3-44415DL594
DyLight 650	NBP3-44415C
DyLight 680	NBP3-44415FR
DyLight 755	NBP3-44415IR
FITC	NBP3-44415F
HRP	NBP3-44415H
Janelia Fluor 525	NBP3-44415JF525
Janelia Fluor 549	NBP3-44415JF549
Janelia Fluor 646	NBP3-44415JF646
Janelia Fluor 669	NBP3-44415JF669
PE	NBP3-44415PE
PerCP	NBP3-44415PCP
mFluor Violet 450 SE	NBP3-44415MFV450
mFluor Violet 500 SE	NBP3-44415MFV500
mFluor Violet 610 SE	NBP3-44415MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44415
BSA Free	NBP2-76501-25ul

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NBP1-89745

Sortilin Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-89745

Immunohistochemistry-Paraffin: Sortilin Antibody [NBP1-89745] - Staining of human hippocampus shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Sortilin Antibody [NBP1-89745] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: Sortilin Antibody [NBP1-89745] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines SK-MEL-30 and PC-3 using Anti-SORT1 antibody. Corresponding SORT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89745-25ul

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3154-ST

Recombinant Human Sortilin Protein, CF

(2 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB31541

Sortilin Antibody (334703) [Unconjugated]

100 μg

MAB31541

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Sortilin Monoclonal Antibody (Catalog # MAB31541, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB31541U-100UG
Alexa Fluor 405	FAB31541V-100UG
Alexa Fluor 488	FAB31541G-100UG
Alexa Fluor 532	FAB31541X
Alexa Fluor 594	FAB31541T-100UG
Alexa Fluor 647	FAB31541R-100UG
Alexa Fluor 700	FAB31541N-100UG
Alexa Fluor 750	FAB31541S-100UG
Alexa Fluor Plus 405	FAB31541AFP405-100UG
Alexa Fluor Plus 488	FAB31541AFP488-100UG
Alexa Fluor Plus 555	FAB31541AFP555-100UG
Alexa Fluor Plus 594	FAB31541AFP594-100UG
Alexa Fluor Plus 647	FAB31541AFP647-100UG
Alexa Fluor Plus 680	FAB31541AFP680-100UG
Allophycocyanin	FAB31541A
Allophycocyanin/Cy7	FAB31541APCCY7
Biotin	FAB31541B
CoraFluor 1	FAB31541CL1
DyLight 350	FAB31541D
DyLight 405	FAB31541E
DyLight 488	FAB31541K
DyLight 550	FAB31541L
DyLight 594	FAB31541M
DyLight 650	FAB31541W
DyLight 680	FAB31541Y
DyLight 755	FAB31541Z
FITC	FAB31541F
HRP	FAB31541H
Janelia Fluor 525	FAB31541JF525
Janelia Fluor 549	FAB31541I
Janelia Fluor 646	FAB31541J
Janelia Fluor 669	FAB31541JF669
PE	FAB31541P
PE/Atto594	FAB31541PEATT594
PE/Cy5.5	FAB31541PECY55
PE/Cy7	FAB31541PECY7
PerCP	FAB31541C
mFluor Violet 450 SE	FAB31541MFV450
mFluor Violet 500 SE	FAB31541MFV500
mFluor Violet 610 SE	FAB31541MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB31541-SP
Carrier Free	FAB31541V-100UG
Carrier Free	FAB31541U-100UG
Carrier Free	FAB31541T-100UG
Carrier Free	FAB31541R-100UG
Carrier Free	FAB31541S-100UG
Carrier Free	FAB31541N-100UG
Carrier Free	FAB31541G-100UG

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2934-ST

Recombinant Mouse Sortilin Protein, CF

(1 Publication)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP3-15095

Sortilin Antibody (S02-6B4)

100 ul

NBP3-15095

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-32968

Sortilin Antibody (JE60-00)

100 ul

NBP3-32968

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 6272

Sortilin Antibody [Unconjugated]

Sortilin Antibody [Unconjugated]

Sortilin Antibody (334708) [Unconjugated]

Sortilin Antibody (CL6526) - BSA Free

Sortilin Antibody (CL6526)

Sortilin Antibody (CL6528) - BSA Free

Sortilin Antibody - BSA Free

Recombinant Human Sortilin Protein, CF

Sortilin Antibody (334703) [Unconjugated]

Recombinant Mouse Sortilin Protein, CF

Sortilin Antibody (S02-6B4)

Sortilin Antibody (JE60-00)