HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC-PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC-PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC-PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1930-SP | |||
Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TC-PTP at approximately 45 to 48 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1930-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48841-25ul | |||
![Western Blot: TC-PTP/PTPN2 Antibody [NBP2-48528] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Western-Blot-NBP2-48528-img0002.jpg "Western Blot: TC-PTP/PTPN2 Antibody [NBP2-48528] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: TC-PTP/PTPN2 Antibody [NBP2-48528] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48528-img0004.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human kidney.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0009.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human colon, kidney, lymph node and testis using Anti-PTPN2 antibody NBP2-48528 (A) shows similar protein distribution across tissues to independent antibody NBP2-48841 (B).](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0006.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human testis.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0007.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human colon.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0008.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human lymph node.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48528-img0010.jpg)
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human colon, kidney, lymph node and testis using Anti-PTPN2 antibody NBP2-48841 (A) shows similar protein distribution across tissues to independent antibody NBP2-48528 (B).](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48841-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human colon, kidney, lymph node and testis using Anti-PTPN2 antibody NBP2-48841 (A) shows similar protein distribution across tissues to independent antibody NBP2-48528 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: TC-PTP/PTPN2 Antibody [NBP2-48841] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Western-Blot-NBP2-48841-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48841-img0004.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human lymph node.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48841-img0009.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human colon.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48841-img0007.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human kidney.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48841-img0008.jpg)
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48841] - Staining of human testis.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48841-img0010.jpg)
![Western Blot: TC-PTP/PTPN2 Antibody [NBP1-60066] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Western-Blot-NBP1-60066-img0001.jpg "Western Blot: TC-PTP/PTPN2 Antibody [NBP1-60066] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP1-60066] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Immunohistochemistry-Paraffin-NBP1-60066-img0002.jpg)
![Western Blot: TC-PTP/PTPN2 Antibody [NBP1-60093] - Jurkat cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Western-Blot-NBP1-60093-img0002.jpg "Western Blot: TC-PTP/PTPN2 Antibody [NBP1-60093] - Jurkat cell lysate, concentration 2.5 ug/ml.")
![Western Blot: TC-PTP/PTPN2 Antibody [NBP1-60093] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 5.0ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/TC-PTP-PTPN2-Antibody-Western-Blot-NBP1-60093-img0003.jpg)
![Western Blot: PTPN2 Overexpression Lysate (Adult Normal) [NBL1-14972] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN2.](http://images.novusbio.com/fullsize/PTPN2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14972-img0002.jpg "Western Blot: PTPN2 Overexpression Lysate (Adult Normal) [NBL1-14972] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN2.")
![Immunoprecipitation:TC-PTP/PTPN2 AntibodyNBP3-29575] -Detection of human PTPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN2 antibody NBP3-29575 (lot NBP3-29575-1) used for IP at 6 µg per reaction. PTPN2 was also immunoprecipitated by rabbit anti-PTPN2.. For blotting immunoprecipitated PTPN2, NBP3-29575 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29575_rabbit-tc-ptp-ptpn2-pab-301220241945459.jpg "Immunoprecipitation:TC-PTP/PTPN2 AntibodyNBP3-29575] -Detection of human PTPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN2 antibody NBP3-29575 (lot NBP3-29575-1) used for IP at 6 µg per reaction. PTPN2 was also immunoprecipitated by rabbit anti-PTPN2.. For blotting immunoprecipitated PTPN2, NBP3-29575 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: PTPN2 Antibody [NBP1-59509] - ACHN cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PTPN2-Antibody-Western-Blot-NBP1-59509-img0002.jpg "Western Blot: PTPN2 Antibody [NBP1-59509] - ACHN cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PTPN2 Overexpression Lysate (Adult Normal) [NBP2-08070] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN2.](http://images.novusbio.com/fullsize/PTPN2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08070-img0001.jpg "Western Blot: PTPN2 Overexpression Lysate (Adult Normal) [NBP2-08070] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN2.")
![Immunoprecipitation:TC-PTP/PTPN2 AntibodyNBP3-29903] -Detection of human PTPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN2 antibody NBP3-29903 (lot NBP3-29903-1) used for IP at 6 µg per reaction. PTPN2 was also immunoprecipitated by rabbit anti-PTPN2.. For blotting immunoprecipitated PTPN2, NBP3-29903 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-29903_rabbit-tc-ptp-ptpn2-pab-3012202419454518.jpg "Immunoprecipitation:TC-PTP/PTPN2 AntibodyNBP3-29903] -Detection of human PTPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN2 antibody NBP3-29903 (lot NBP3-29903-1) used for IP at 6 µg per reaction. PTPN2 was also immunoprecipitated by rabbit anti-PTPN2.. For blotting immunoprecipitated PTPN2, NBP3-29903 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
