TC-PTP/PTPN2 Overexpression Lysate (Native)


Western Blot: PTPN2 Overexpression Lysate (Adult Normal) [NBL1-14972] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

TC-PTP/PTPN2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC205090 and based on accession number NM_080423. The protein contains a C-terminal DDK tag.
Homo sapiens protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 3, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TC-PTP/PTPN2 Overexpression Lysate (Native)

  • protein tyrosine phosphatase, non-receptor type 2
  • PTP2
  • PTPN2
  • T-cell protein tyrosine phosphatase
  • T-cell protein-tyrosine phosphatase
  • TC-PTP
  • tyrosine-protein phosphatase non-receptor type 2


The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of this gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms. Two highly related but distinctly processed pseudogenes that localize to distinct chromosomes have been reported. [provided by RefSeq]. Transcript Variant: This variant (3) contains an alternate 3' region, which includes a part of the C-terminal coding region, when compared to variant 1. The resulting protein lacks the C-terminal 67 aa, and contains an unique 6 aa C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, IHC
Species: Hu, Rt
Applications: WB
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Rt, Hu(-)
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow, KO
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Species: Hu
Applications: WB, IHC
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow

Publications for TC-PTP/PTPN2 Lysate (NBL1-14972) (0)

There are no publications for TC-PTP/PTPN2 Lysate (NBL1-14972).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TC-PTP/PTPN2 Lysate (NBL1-14972) (0)

There are no reviews for TC-PTP/PTPN2 Lysate (NBL1-14972). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TC-PTP/PTPN2 Lysate (NBL1-14972) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for TC-PTP/PTPN2 Lysate (NBL1-14972)

Discover related pathways, diseases and genes to TC-PTP/PTPN2 Lysate (NBL1-14972). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TC-PTP/PTPN2 Lysate (NBL1-14972)

Discover more about diseases related to TC-PTP/PTPN2 Lysate (NBL1-14972).

Pathways for TC-PTP/PTPN2 Lysate (NBL1-14972)

View related products by pathway.

PTMs for TC-PTP/PTPN2 Lysate (NBL1-14972)

Learn more about PTMs related to TC-PTP/PTPN2 Lysate (NBL1-14972).

Blogs on TC-PTP/PTPN2

There are no specific blogs for TC-PTP/PTPN2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-6 [Unconjugated]

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TC-PTP/PTPN2 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol PTPN2