| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-201SS | |||
| Azide and BSA Free | NBP2-80911 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5340-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87268-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5340-SP | |||
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunocytochemistry-Immunofluorescence-NB600-201-img0001.jpg "Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha detected in muse prostate tissue using PKC alpha Antibody (MC5). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunohistochemistry-Paraffin-NB600-201-img0004.jpg)
![Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Flow-Cytometry-NB600-201-img0003.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunohistochemistry-Paraffin-NB600-201-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940840.jpg "Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024941594.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249342742.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249422634.jpg)
![Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249415946.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940812.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249383155.jpg)
![Western Blot: PKC alpha Antibody [NBP1-87269] - Analysis using Anti-PRKCA antibody NBP1-87269 (A) shows similar pattern to independent antibody NBP1-87268 (B).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87269-img0014.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PKC alpha Antibody [NBP1-87269] - Analysis using Anti-PRKCA antibody NBP1-87269 (A) shows similar pattern to independent antibody NBP1-87268 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PKC alpha Antibody [NBP1-87269] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87269-img0012.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87269] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-87269-img0011.jpg)
![Western Blot: PKC alpha Antibody [NBP1-87269] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87269-img0004.jpg)
![Western Blot: PKC alpha Antibody [NBP1-87269] - Lane 1: Marker {kDA] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Huan Cerebral Cortex tissue.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87269-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: PKC alpha Antibody [NBP1-87269] - Analysis in human cell lines A-549 and Caco-2 using anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87269-img0013.jpg)
![Western Blot: PKC alpha Antibody [NBP1-87268] - Analysis using Anti-PRKCA antibody NBP1-87268 (A) shows similar pattern to independent antibody NBP1-87269 (B).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87268-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PKC alpha Antibody [NBP1-87268] - Analysis using Anti-PRKCA antibody NBP1-87268 (A) shows similar pattern to independent antibody NBP1-87269 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PKC alpha Antibody [NBP1-87268] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87268-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87268] - Staining in human cerebral cortex and pancreas tissues using anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-87268-img0010.jpg)
![Western Blot: PKC alpha Antibody [NBP1-87268] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Western-Blot-NBP1-87268-img0006.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87268] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-87268-img0008.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87268] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-87268-img0009.jpg)
![Western Blot: PKC alpha Antibody (2F11) [H00005578-M01] - PRKCA monoclonal antibody (M01), clone 2F11 Analysis of PRKCA expression in HeLa.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Western-Blot-H00005578-M01-img0011.jpg "Western Blot: PKC alpha Antibody (2F11) [H00005578-M01] - PRKCA monoclonal antibody (M01), clone 2F11 Analysis of PRKCA expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00005578-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Immunohistochemistry-Paraffin-H00005578-M01-img0009.jpg)
![Proximity Ligation Assay: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of protein-protein interactions between FAS and PRKCA. HeLa cells were stained with anti-FAS rabbit purified polyclonal 1:1200 and anti-PRKCA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Proximity-Ligation-Assay-H00005578-M01-img0005.jpg)
![ELISA: PKC alpha Antibody (2F11) [H00005578-M01] - Detection limit for recombinant GST tagged PRKCA is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-ELISA-H00005578-M01-img0012.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25604_rabbit-pan-pkc-p-thr-410-mab-hl1278-azide-and-bsa-free-2120249355560.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25604_rabbit-pan-pkc-p-thr-410-mab-hl1278-azide-and-bsa-free-2120249425470.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25604_rabbit-pan-pkc-p-thr-410-mab-hl1278-azide-and-bsa-free-212024941590.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25604_rabbit-pan-pkc-p-thr-410-mab-hl1278-azide-and-bsa-free-212024937159.jpg)
![Western Blot: pan PKC [p Thr410] Antibody (HL1278) - Azide and BSA Free [NBP3-25604] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (NBP3-25604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25604_rabbit-pan-pkc-p-thr-410-mab-hl1278-azide-and-bsa-free-2120249383156.jpg)
![Western Blot: pan PKC Antibody [NBP3-05673] - Analysis of extracts of various cell lines, using pan PKC antibody (NBP3-05673) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/pan-PKC-Antibody-Western-Blot-NBP3-05673-img0002.jpg "Western Blot: pan PKC Antibody [NBP3-05673] - Analysis of extracts of various cell lines, using pan PKC antibody (NBP3-05673) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: pan PKC Antibody [NBP3-05673] - Immunofluorescence analysis of NIH-3T3 cells using pan PKC Rabbit pAb (NBP3-05673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/pan-PKC-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05673-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: pan PKC Antibody - BSA Free [pan PKC] - Immunofluorescence analysis of NIH-3T3 cells using pan PKC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05673_rabbit-polyclonal-pan-pkc-antibody-5220251726080.jpg)
![Western Blot: PKC alpha Antibody (M237) [NBP3-23297] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.](http://images.novusbio.com/fullsize/nbp3-23297_mouse-pkc-alpha-mab-m237-1992023191549.jpg "Western Blot: PKC alpha Antibody (M237) [NBP3-23297] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.")
![Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231759448.jpg "Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231856932.jpg)
![Western Blot: PKC alpha Antibody (G13-R) [NBP1-30121] - Lane 1-50 ng; lane 2-100 ng; lane 3 -200 ng of recombinant human.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-G13-R-Western-Blot-NBP1-30121-img0003.jpg "Western Blot: PKC alpha Antibody (G13-R) [NBP1-30121] - Lane 1-50 ng; lane 2-100 ng; lane 3 -200 ng of recombinant human.")
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (G13-R) [NBP1-30121] - Expression in HEK293 cells, visualized with dilution - 1:100.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-G13-R-Immunocytochemistry-Immunofluorescence-NBP1-30121-img0004.jpg)