| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22127SS | |||
| Azide and BSA Free | NBP2-80575 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3197-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | DYC3197-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3197-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05635-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49430-25ul | |||
![Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Simple-Western-NBP2-22127-img0006.jpg "Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunocytochemistry-Immunofluorescence-NBP2-22127-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and 72 hours following exposure to 0.4 uM GNE-617. Full-length western blots are shown in S2D Fig. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0164166), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0009.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunohistochemistry-Paraffin-NBP2-22127-img0005.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0008.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0007.jpg)
![ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-ELISA-NBP2-22127-img0001.jpg)
![Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NB100-239-img0006.jpg "Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (NB100-239). Detection: DAB](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NB100-239-img0005.jpg)
![Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 (lot 2) used for IP at 3 ug per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AMPK alpha 1, NB100-239 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunoprecipitation-NB100-239-img0007.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Western-Blot-NB100-81784-img0016.jpg "Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Paraffin-embedded human colon carcinoma tissue using NB100-81784 or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81784_rabbit-polyclonal-ampk-alpha-1-p-ser496-antibody-10320252346459.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using NB100-81784.](http://images.novusbio.com/fullsize/nb100-81784_rabbit-polyclonal-ampk-alpha-1-p-ser496-antibody-10320252347269.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Analysis of extracts from HUVEC cells untreated or treated with UV using NB100-81784](http://images.novusbio.com/fullsize/nb100-81784_rabbit-polyclonal-ampk-alpha-1-p-ser496-antibody-103202523472624.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81784.](http://images.novusbio.com/fullsize/nb100-81784_rabbit-polyclonal-ampk-alpha-1-p-ser496-antibody-103202523321011.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0003.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded rat lung using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of PC-12 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0002.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded human colon using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0004.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded mouse kidney using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0005.jpg)
![Western Blot: AMPK alpha 1/2 Antibody [NBP3-05628] -Analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 45s.](http://images.novusbio.com/fullsize/antibody/nbp3-05628_rabbit-polyclonal-ampk-alpha-1-2-antibody-western-blot-2312025104326..png)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [AMPK alpha 1/2] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05628_rabbit-polyclonal-ampk-alpha-1-2-antibody-522025163637.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [AMPK alpha 1/2] - Immunofluorescence analysis of MCF7 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05628_rabbit-polyclonal-ampk-alpha-1-2-antibody-52202516321458.jpg)
![ELISA AMPK alpha 1 [Biotin]](http://images.novusbio.com/fullsize2/dyc3197-2_human-mouse-rat-total-ampk-alpha-1-duoset-ic-elisa-2-plate-elisa-159202015841.jpg "ELISA AMPK alpha 1 [Biotin]")
![Western Blot: AMPK alpha 1/2 Antibody [NBP3-05635] - Western blot analysis of extracts of various cell lines, using AMPK alpha 1/2 antibody (NBP3-05635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AMPK alpha 1-2 Antibody-Western Blot-NBP3-05635-img0004.jpg "Western Blot: AMPK alpha 1/2 Antibody [NBP3-05635] - Western blot analysis of extracts of various cell lines, using AMPK alpha 1/2 antibody (NBP3-05635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635] - Western blot analysis of 293F, using AMPK alpha 1/2 antibody (A17290) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05635_rabbit-polyclonal-ampk-alpha-1-2-antibody-52202519294552.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05635_rabbit-polyclonal-ampk-alpha-1-2-antibody-72202512432024.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635] - Immunofluorescence analysis of PC-12 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05635_rabbit-polyclonal-ampk-alpha-1-2-antibody-72202512435349.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05635_rabbit-polyclonal-ampk-alpha-1-2-antibody-7220251243533.jpg)
![Western Blot: AMPK alpha 1/2 Antibody [NBP3-12281] - Mouse brain. 1:1000 antibody dilution in Dilobuffer. Apparent MW is 62 KDa.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Western-Blot-NBP3-12281-img0002.jpg "Western Blot: AMPK alpha 1/2 Antibody [NBP3-12281] - Mouse brain. 1:1000 antibody dilution in Dilobuffer. Apparent MW is 62 KDa.")
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-12281] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12281-img0001.jpg)
![Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] - Western blot analysis of lysates from C2C12 cells, using AMPK alpha 1/2 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated by mHGF(50ng/uL).Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33579_rabbit-ampk-alpha-1-2-p-thr172-p-thr183-mab-2o0p9-101220241243946.jpg "Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] - Western blot analysis of lysates from C2C12 cells, using AMPK alpha 1/2 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated by mHGF(50ng/uL).Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] - Western blot analysis of lysates from C6 cells, using AMPK alpha 1/2 Rabbit mAb at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33579_rabbit-ampk-alpha-1-2-p-thr172-p-thr183-mab-2o0p9-1012202411552820.jpg)
![Western Blot: AMPK alpha 1 Antibody [NBP2-49430] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NBP2-49430-img0002.jpg "Western Blot: AMPK alpha 1 Antibody [NBP2-49430] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry: AMPK alpha 1 Antibody [NBP2-49430] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NBP2-49430-img0001.jpg)