| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83670-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59389-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99440-50ul | |||
![Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Otubain-1.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Western-Blot-NBP2-45879-img0012.jpg "Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Otubain-1.")
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0011.jpg)
![Flow Cytometry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by OTUB1 antibody.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Flow-Cytometry-NBP2-45879-img0001.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0002.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0003.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0004.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0005.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0006.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human tonsil tissue within the normal limits using OTUB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0007.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0008.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human pancreas tissue .](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0009.jpg)
![Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-OTI5B8-Immunohistochemistry-NBP2-45879-img0010.jpg)
![Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-49934-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-GT611-Western-Blot-NBP3-13627-img0001.jpg "Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627] - Otubain-1 antibody [GT611] detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-GT611-Immunocytochemistry-Immunofluorescence-NBP3-13627-img0002.jpg)
![Western Blot: Otubain-1 Antibody [NBP3-13214] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody (NBP3-13214) diluted at 1:1000.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP3-13214-img0001.jpg "Western Blot: Otubain-1 Antibody [NBP3-13214] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody (NBP3-13214) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody [NBP3-13214] - Otubain-1 antibody detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody (NBP3-13214) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13214-img0002.jpg)
![Western Blot: Otubain-1 Antibody (17E10) [NBP2-59389] - Lane 1: A549 cell lysates, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: 293T cell lysates](http://images.novusbio.com/fullsize/Otubain-1-Antibody-17E10-Western-Blot-NBP2-59389-img0001.jpg "Western Blot: Otubain-1 Antibody (17E10) [NBP2-59389] - Lane 1: A549 cell lysates, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (17E10) [NBP2-59389] - Analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-17E10-Immunocytochemistry-Immunofluorescence-NBP2-59389-img0002.jpg)
![Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09] - Analysis of OTUB1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-1C12-Western-Blot-H00055611-M09-img0003.jpg "Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09] - Analysis of OTUB1 expression in Hela S3 NE (Cat # L013V3).")
![ELISA: Otubain-1 Antibody (1C12) [H00055611-M09] - Detection limit for recombinant GST tagged OTUB1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-1C12-ELISA-H00055611-M09-img0004.jpg)
![Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-20957-img0002.jpg "Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Otubain-1 Antibody [NBP1-49935] - OTUB1 Antibody [NBP1-49935] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP1-49935-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Otubain-1 Antibody [NBP1-49935] - OTUB1 Antibody [NBP1-49935] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: OTUB1 Overexpression Lysate (Adult Normal) [NBL1-14011] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OTUB1.](http://images.novusbio.com/fullsize/OTUB1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14011-img0002.jpg "Western Blot: OTUB1 Overexpression Lysate (Adult Normal) [NBL1-14011] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OTUB1.")
![Western Blot: Otubain-1 Antibody [NBP2-99440] - Anti-Otubain-1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/Otubain-1-Antibody-Western-Blot-NBP2-99440-img0002.jpg "Western Blot: Otubain-1 Antibody [NBP2-99440] - Anti-Otubain-1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa")