Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ma,
RbSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ma, Rb
WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Applications: WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(2G11)-Immunocytochemistry-Immunofluorescence-NB300-583-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(2G11)-Immunohistochemistry-Paraffin-NB300-583-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(2G11)-Immunocytochemistry-Immunofluorescence-NB300-583-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(2G11)-Immunocytochemistry-Immunofluorescence-NB300-583-img0009.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(2G11)-Immunohistochemistry-Paraffin-NB300-583-img0006.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.](http://images.novusbio.com/fullsize2/Hydrogen Potassium ATPase Beta Antibody (2G11)-Immunohistochemistry-Paraffin-NB300-583-img0012.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Positive staining can be observed in human gastric parietal cells using this antibody. Red is Hydrogen Potassium ATPase Beta. Image from verified customer review.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0012.jpg "Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Positive staining can be observed in human gastric parietal cells using this antibody. Red is Hydrogen Potassium ATPase Beta. Image from verified customer review.")
![Immunohistochemistry: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human stomach, lower shows strong cytoplasmic positivity in subset of glandular cells.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-NBP2-32035-img0004.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0005.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining in human stomach and pancreas tissues using anti-ATP4B antibody. Corresponding ATP4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human colon, kidney, liver and stomach using Anti-ATP4B antibody NBP2-32035 (A) shows similar protein distribution across tissues to independent antibody NBP2-38674 (B).](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0008.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human kidney.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0009.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human liver.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0010.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human colon.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0011.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human liver.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0008.jpg "Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human liver.")
![Immunohistochemistry: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-NBP2-38674-img0001.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0002.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining in human stomach and pancreas tissues using anti-ATP4B antibody. Corresponding ATP4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human colon, kidney, liver and stomach using Anti-ATP4B antibody NBP2-38674 (A) shows similar protein distribution across tissues to independent antibody NBP2-32035 (B).](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0005.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human kidney.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0006.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-38674] - Staining of human colon.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-38674-img0007.jpg)
![Western Blot: Hydrogen Potassium ATPase Beta Antibody (3Y1Y1) [NBP3-15739] - Western blot analysis of extracts of various cell lines, using Hydrogen Potassium ATPase Beta antibody (NBP3-15739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(3Y1Y1)-Western-Blot-NBP3-15739-img0003.jpg "Western Blot: Hydrogen Potassium ATPase Beta Antibody (3Y1Y1) [NBP3-15739] - Western blot analysis of extracts of various cell lines, using Hydrogen Potassium ATPase Beta antibody (NBP3-15739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (3Y1Y1) [NBP3-15739] - Immunohistochemistry of paraffin-embedded mouse stomach using Hydrogen Potassium ATPase Beta Rabbit mAb (NBP3-15739) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(3Y1Y1)-Immunohistochemistry-Paraffin-NBP3-15739-img0002.jpg)
![Western Blot: Hydrogen Potassium ATPase Beta Antibody (3Y1Y1) [NBP3-15739] - Western blot analysis of extracts of Mouse stomach, using Hydrogen Potassium ATPase Beta antibody (NBP3-15739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize2/Hydrogen Potassium ATPase Beta Antibody (3Y1Y1)-Western Blot-NBP3-15739-img0004.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (3Y1Y1) [NBP3-15739] - Immunohistochemistry of paraffin-embedded rat stomach using Hydrogen Potassium ATPase Beta Rabbit mAb (NBP3-15739) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(3Y1Y1)-Immunohistochemistry-Paraffin-NBP3-15739-img0001.jpg)
![Western Blot: Hydrogen Potassium ATPase Beta Antibody [NBP1-60028] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/Hydrogen Potassium ATPase Beta Antibody-Western Blot-NBP1-60028-img0002.jpg "Western Blot: Hydrogen Potassium ATPase Beta Antibody [NBP1-60028] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![SDS-Page: Recombinant Human Hydrogen Potassium ATPase Beta Protein [H00000496-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-Hydrogen-Potassium-ATPase-Beta-Protein-SDS-Page-H00000496-Q01-img0001.jpg "SDS-Page: Recombinant Human Hydrogen Potassium ATPase Beta Protein [H00000496-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Hydrogen Potassium ATPase Beta Protein [H00000496-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-Hydrogen-Potassium-ATPase-Beta-Protein-SDS-Page-H00000496-P01-img0001.jpg "SDS-Page: Recombinant Human Hydrogen Potassium ATPase Beta Protein [H00000496-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Hydrogen Potassium ATPase Beta Antibody [NBP1-91573] - Rat Lung lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-Western-Blot-NBP1-91573-img0003.jpg "Western Blot: Hydrogen Potassium ATPase Beta Antibody [NBP1-91573] - Rat Lung lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Hydrogen Potassium ATPase Beta Overexpression Lysate (Adult Normal) [NBL1-07815] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hydrogen Potassium ATPase Beta.](http://images.novusbio.com/fullsize/Hydrogen Potassium ATPase Beta Overexpression Lysate (Adult Normal)-Western Blot-NBL1-07815-img0002.jpg "Western Blot: Hydrogen Potassium ATPase Beta Overexpression Lysate (Adult Normal) [NBL1-07815] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hydrogen Potassium ATPase Beta.")
![ELISA: Hydrogen Potassium ATPase Beta Antibody (1D10) [H00000496-M10] - Detection limit for recombinant GST tagged ATP4B is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/Hydrogen-Potassium-ATPase-Beta-Antibody-(1D10)-ELISA-H00000496-M10-img0001.jpg "ELISA: Hydrogen Potassium ATPase Beta Antibody (1D10) [H00000496-M10] - Detection limit for recombinant GST tagged ATP4B is approximately 0.1ng/ml as a capture antibody.")