| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50612-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05219-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54669-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47970-0.1mg | |||
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22162-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48293 | |||
| Azide and BSA Free | NBP3-11576 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22165-SP | |||
![Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-50612-img0002.jpg "Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50612-img0001.jpg)
![Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0006.jpg "Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05219-img0004.jpg)
![Immunohistochemistry Free-Floating: NF-L Antibody [NBP1-05219] - Staining of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP1-05219 antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494437..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-L Antibody [NBP2-54669] - Analysis in control (vector only transfected HEK293T lysate) and NEFL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-54669-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494530..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebellum shows strong positivity in neuronal processes in molecular layer.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494628..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494721..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494818..jpg)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Immunohistochemistry-Paraffin-NBP2-44893-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) [NBP2-44893] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Flow-Cytometry-NBP2-44893-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).](http://images.novusbio.com/fullsize/NF-L-Antibody-SPM204-Immunohistochemistry-Paraffin-NBP2-44892-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).")
![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-132202416102266.jpg "ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-432024941537.jpg)
