| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44128 | |||
| BSA Free | NBP2-59061-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2216-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22164-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37528-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15874-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50612-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22163-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05219-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37525-0.025ml | |||
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)
![Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Western-Blot-NB300-132-img0003.jpg "Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Immunocytochemistry-Immunofluorescence-NB300-132-img0004.jpg)
![Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Simple-Western-NB300-132-img0005.jpg)
![Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Western-Blot-NBP2-59061-img0008.jpg "Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061] - Staining of PC-3 cells using the Anti-NEFL monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunocytochemistry-Immunofluorescence-NBP2-59061-img0010.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0009.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0001.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0003.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0004.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human liver shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0007.jpg)
![Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Western-Blot-NBP2-37528-img0003.jpg "Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (1H3) [NBP2-37528] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunocytochemistry-Immunofluorescence-NBP2-37528-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (1H3) [NBP2-37528] - Analysis of bladder cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunohistochemistry-NBP2-37528-img0007.jpg)
![Flow Cytometry: NF-L Antibody (1H3) [NBP2-37528] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Flow-Cytometry-NBP2-37528-img0005.jpg)
![Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Western-Blot-NBP2-37528-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (1H3) [NBP2-37528] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunohistochemistry-NBP2-37528-img0006.jpg)
![ELISA: NF-L Antibody (1H3) [NBP2-37528] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-ELISA-NBP2-37528-img0001.jpg)
![Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235717.jpg "Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235788.jpg)
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517152736.jpg)
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-5220251721355.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517172848.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of Human brain tissue using NF-L Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517162336.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Mouse brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517184520.jpg)
![Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-50612-img0002.jpg "Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50612-img0001.jpg)
![Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0006.jpg "Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05219-img0004.jpg)
![Immunohistochemistry Free-Floating: NF-L Antibody [NBP1-05219] - Staining of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP1-05219 antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0005.jpg)
![Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Western-Blot-NBP2-37525-img0002.jpg "Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37525-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of brain tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0006.jpg)
![Flow Cytometry: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Flow-Cytometry-NBP2-37525-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0005.jpg)
![ELISA: NF-L Antibody (2G10) [NBP2-37525] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-ELISA-NBP2-37525-img0001.jpg)