| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22711SS | |||
| Azide and BSA Free | NBP2-80859 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-60046SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72826 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47888-0.1mg | |||
| IHC-Prediluted | NBP2-48378 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47884-0.1mg | |||
| IHC-Prediluted | NBP2-48360 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47886-0.1mg | |||
| IHC-Prediluted | NBP2-48375 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44662 | |||
| Azide and BSA Free | NBP2-34737-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47885-0.1mg | |||
| IHC-Prediluted | NBP2-48361 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62565-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47883-0.1mg | |||
| IHC-Prediluted | NBP2-48359 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33174 | |||
| IHC-Prediluted | NBP2-44661 | |||
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunocytochemistry-Immunofluorescence-NB120-22711-img0004.jpg "Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunohistochemistry-Paraffin-NB120-22711-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (SM3) - BSA Free [NB120-22711] - An intracellular stain was performed on MCF7 cells with MUC1 Antibody (SM3) NB120-22711AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-BSA-Free-Flow-Cytometry-NB120-22711-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunocytochemistry-Immunofluorescence-NB120-22711-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong diffused immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunohistochemistry-Paraffin-NB120-22711-img0002.jpg)
![Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 Antibody [NBP1-60046] - C57BL/6 Muc1 KO mice and WT mice were undergoing intranasal instillation of 75 ug of lipopolysaccharide (LPS) at day 1. Between days 2 and 3, animals were exposed to cigarette smoke (or control air) of 6 cigarettes followed by 8 cigarettes (or control air) at days 4 and 5, and 10 cigarettes (or control air) at day 6. Vehicle or dexamethasone at 3 mg/kg/day and 10 mg/kg/day was administered orally once a day between day 1 and 6. Animals were sacrificed at day 6 and lungs were homogenized to analyze protein expression of Muc1 cytoplasmic tail (CT). Protein expression was normalized to B-actin. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-018-0927-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-Knockout-Validated-NBP1-60046-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 Antibody [NBP1-60046] - C57BL/6 Muc1 KO mice and WT mice were undergoing intranasal instillation of 75 ug of lipopolysaccharide (LPS) at day 1. Between days 2 and 3, animals were exposed to cigarette smoke (or control air) of 6 cigarettes followed by 8 cigarettes (or control air) at days 4 and 5, and 10 cigarettes (or control air) at day 6. Vehicle or dexamethasone at 3 mg/kg/day and 10 mg/kg/day was administered orally once a day between day 1 and 6. Animals were sacrificed at day 6 and lungs were homogenized to analyze protein expression of Muc1 cytoplasmic tail (CT). Protein expression was normalized to B-actin. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-018-0927-4) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: MUC1 Antibody [NBP1-60046] - Dexamethasone induces Muc1-CT nuclear translocation and co-localization with GRa in bronchial epithelium of WT mice but not in MUC1 KO mice. C57BL/6 Muc1 KO mice and WT mice were undergoing dexamethasone 10 mg/kg/day (orally) for 6 days. Animals were sacrificed at day 6 and lungs were fixed in paraformaldehyde (4%) for 48 h and embedded. Lung slices were immunostained with MUC1-cytoplasmic tail (CT) (red) and glucocorticoid receptor alpha (GRa) antibodies (green) with rhodamine/fluorescein secondary antibodies. DAPI was used to mark cell nucleus. Representative con-focal images are showed. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-018-0927-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-Knockout-Validated-NBP1-60046-img0013.jpg)
![Western Blot: MUC-1 Antibody [NBP1-60046] - Total protein from mouse 3T3 cells and human HeLa cells, stomach and colon was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MUC-1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MUC-1-Antibody-Western-Blot-NBP1-60046-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody [NBP1-60046] - Tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Cells were counterstained for alpha-tubulin against DyLight 550 (Red) and mounted in DAPI fluoromount (Blue).](http://images.novusbio.com/fullsize/MUC-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-60046-img0010.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0008.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046] - Dexamethasone induces Muc1-CT nuclear translocation & co-localization with GR alpha in bronchial epithelium of WT mice but not in MUC1 KO mice. C57BL/6 Muc1 KO mice & WT mice were undergoing dexamethasone 10 mg/kg/day (orally) for 6 days. Animals were sacrificed at day 6 & lungs were fixed in paraformaldehyde (4%) for 48 h & embedded in Tissue-Tek® OCT™ cryosectioning compound. Lung slices were immunostained with MUC1-cytoplasmic tail (CT) & glucocorticoid receptor alpha (GR alpha ) antibodies with rhodamine/fluorescein secondary antibodies. DAPI was used to mark cell nucleus. Representative con-focal images are showed. Scale bar: 5 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202415363741.jpg)
![Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416161843.jpg)
![Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 downregulation & TLR4 overexpression in lung tissue from heavy smokers & COPD patients. Lung tissue from healthy (n = 10), smokers (n = 11) & COPD patients (n = 13) were analysed. MUC1 mRNA gene expression in lung tissue homogenates (a), bronchial epithelial cells (b) & sputum neutrophils (c). d MUC1-CT protein expression in lung homogenates.TLR4 mRNA gene expression in lung tissue homogenates (e), bronchial epithelial cells (f) & sputum neutrophils (g). h MUC1-CT protein expression in lung homogenates. i Correlation of TL4 & MUC1 gene expression in lung tissue from smokers & COPD patients. j Correlation of FEV1% & MUC1 gene expression in lung tissue from smokers & COPD patients. Gene expression was analyzed by real time PCR using the 2-delta Ct as described in methods. Protein expression was analyzed by western blot. Representative western blot are showed. Data are presented as median with interquartile range. “P” exact values were obtained following Kruskal Wallis test. FEV1%: forced expiratory volume in 1 s Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416165614.jpg)
![Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416175235.jpg)
![Western Blot: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of extracts (35ug) from 9 different cell lines by using MUC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Western-Blot-NBP2-45838-img0010.jpg "Western Blot: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of extracts (35ug) from 9 different cell lines by using MUC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0009.jpg)
![Western Blot: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MUC-1.](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Western-Blot-NBP2-45838-img0011.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0001.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0002.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0003.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0004.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0005.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0006.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0007.jpg)
![Immunohistochemistry: MUC-1 Antibody (2E3) [NBP2-45838] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MUC-1-Antibody-2E3-Immunohistochemistry-NBP2-45838-img0008.jpg)
![Western Blot: MUC1 Antibody (139H2) [NBP2-44666] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (139H2).](http://images.novusbio.com/fullsize/MUC1-Antibody-139H2-Western-Blot-NBP2-44666-img0005.jpg "Western Blot: MUC1 Antibody (139H2) [NBP2-44666] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (139H2).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (139H2) [NBP2-44666] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (139H2).](http://images.novusbio.com/fullsize/MUC1-Antibody-139H2-Immunohistochemistry-Paraffin-NBP2-44666-img0002.jpg)
![Western Blot: MUC1 Antibody (139H2) [NBP2-44666] - Western Blot Analysis of human T47D cell lysate using MUC1 Antibody (139H2).](http://images.novusbio.com/fullsize/MUC1-Antibody-139H2-Western-Blot-NBP2-44666-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (139H2) [NBP2-44666] - Human Breast Cancer stained with EMA Monoclonal Antibody (139H2).](http://images.novusbio.com/fullsize/MUC1-Antibody-139H2-Immunohistochemistry-Paraffin-NBP2-44666-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (139H2) [NBP2-44666] - Human Lung Cancer stained with EMA Monoclonal Antibody (139H2).](http://images.novusbio.com/fullsize/MUC1-Antibody-139H2-Immunohistochemistry-Paraffin-NBP2-44666-img0003.jpg)
![Western Blot: MUC1 Antibody (MUC1/955) [NBP2-44658] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/955).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-955-Western-Blot-NBP2-44658-img0005.jpg "Western Blot: MUC1 Antibody (MUC1/955) [NBP2-44658] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/955).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/955) [NBP2-44658] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (MUC1/955).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-955-Immunohistochemistry-Paraffin-NBP2-44658-img0002.jpg)
![Western Blot: MUC1 Antibody (MUC1/955) [NBP2-44658] - Western Blot Analysis of human T47D cell lysate using MUC1 Antibody (MUC1/955).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-955-Western-Blot-NBP2-44658-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/955) [NBP2-44658] - Human Breast Carcinoma stained with EMA Monoclonal Antibody (MUC1/955).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-955-Immunohistochemistry-Paraffin-NBP2-44658-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/955) [NBP2-44658] - Human Lung Carcinoma stained with EMA Monoclonal Antibody (MUC1/955).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-955-Immunohistochemistry-Paraffin-NBP2-44658-img0003.jpg)
![Western Blot: MUC1 Antibody (MUC1/520) [NBP2-44660] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Western-Blot-NBP2-44660-img0007.jpg "Western Blot: MUC1 Antibody (MUC1/520) [NBP2-44660] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/520).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Ovarian Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Colon Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Endometrial Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0005.jpg)
![Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Western-Blot-NBP2-32877-img0003.jpg "Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (SPM132) [NBP2-32877] - Formalin-fixed, paraffin-embedded human Breast cancer stained with EMA Monoclonal Antibody (SPM132)](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Immunohistochemistry-Paraffin-NBP2-32877-img0001.jpg)
![Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MCF-7 cells treated +/- rhIL-8 at 10 ng/ml during 5 days to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Western-Blot-NBP2-32877-img0002.jpg)
![Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Western-Blot-NBP2-44659-img0004.jpg "Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Breast Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Analysis in human stomach and liver tissues using NBP3-05503 antibody. Corresponding MUC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0007.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Analysis in human stomach and liver tissues using NBP3-05503 antibody. Corresponding MUC1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human breast cancer shows strong cytoplasmic positivity.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human Fallopian tube shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (CL8733) [NBP3-05503] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-CL8733-Immunohistochemistry-Paraffin-NBP3-05503-img0006.jpg)
![Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Western-Blot-NBP2-62565-img0001.jpg "Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. HMFG2 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of HMFG2 (NBP2-62565). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62565 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration, 1-2 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunohistochemistry-Paraffin-NBP2-62565-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Flow-cytometry using the anti-MUC1 antibody HMFG2. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of HMFG2 (NBP2-62565, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Flow-Cytometry-NBP2-62565-img0002.jpg)
![Immunofluorescence: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of HMFG2 (NBP2-62565) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62565, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunofluorescence-NBP2-62565-img0004.jpg)
![Western Blot: MUC1 Antibody (HMPV) [NBP2-44657] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 Antibody (HMPV).](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Western-Blot-NBP2-44657-img0005.jpg "Western Blot: MUC1 Antibody (HMPV) [NBP2-44657] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 Antibody (HMPV).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMPV) [NBP2-44657] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (HMPV).](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Immunohistochemistry-Paraffin-NBP2-44657-img0001.jpg)
![Flow Cytometry: MUC1 Antibody (HMPV) [NBP2-44657] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells. MUC1 Antibody (HMPV) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Flow-Cytometry-NBP2-44657-img0003.jpg)
![Western Blot: MUC1 Antibody (GP1.4) [NBP2-29408] - Western Blot Analysis of MCF-7 cell lysate using MUC1 Mouse Monoclonal Antibody (GP1.4).](http://images.novusbio.com/fullsize/MUC1-Antibody-GP1-4-Western-Blot-NBP2-29408-img0005.jpg "Western Blot: MUC1 Antibody (GP1.4) [NBP2-29408] - Western Blot Analysis of MCF-7 cell lysate using MUC1 Mouse Monoclonal Antibody (GP1.4).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (GP1.4) [NBP2-29408] - Formalin-paraffin human breast cancer stained with EMA Ab (GP1.4). Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/MUC1-Antibody-GP1-4-Immunohistochemistry-Paraffin-NBP2-29408-img0002.jpg)