Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Themis Monoclonal Antibody (Catalog # MAB6816) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Themis at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6816-SP | |||
| Carrier Free | IC6816U-100UG | |||
| Carrier Free | IC6816T-100UG | |||
| Carrier Free | IC6816S-100UG | |||
| Carrier Free | IC6816R-100UG | |||
| Carrier Free | IC6816N-100UG | |||
| Carrier Free | IC6816V-100UG | |||
| Carrier Free | IC6816G-100UG | |||
HAF016). A specific band was detected for Themis at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse thymus tissue and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6816) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Themis at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6816-SP | |||
F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human Themis Monoclonal Antibody (Catalog # MAB6929) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6929-SP | |||
| Carrier Free | IC6929S-100UG | |||
| Carrier Free | IC6929R-100UG | |||
| Carrier Free | IC6929N-100UG | |||
| Carrier Free | IC6929V-100UG | |||
| Carrier Free | IC6929U-100UG | |||
| Carrier Free | IC6929T-100UG | |||
| Carrier Free | IC6929G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90802-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26287-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22411-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38183-25ul | |||
MAB0061, open histogram), followed by Phy-coerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ![Western Blot: Themis Antibody [NBP1-90802] - Analysis in human cell line Jurkat.](http://images.novusbio.com/fullsize/Themis-Antibody-Western-Blot-NBP1-90802-img0010.jpg "Western Blot: Themis Antibody [NBP1-90802] - Analysis in human cell line Jurkat.")
![Immunohistochemistry-Paraffin: Themis Antibody [NBP1-90802] - Staining of human thymus shows strong cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/Themis-Antibody-Immunohistochemistry-Paraffin-NBP1-90802-img0012.jpg)
![Immunohistochemistry: Themis Antibody (7G7) [NBP3-26287] - Image of Themis Antibody (7G7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.30% DAB.](http://images.novusbio.com/fullsize/nbp3-26287_rabbit-themis-mab-7g7-2722024827499.jpg "Immunohistochemistry: Themis Antibody (7G7) [NBP3-26287] - Image of Themis Antibody (7G7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.30% DAB.")
![Immunocytochemistry/Immunofluorescence: Themis Antibody (7G7) [NBP3-26287] - Staining of HepG2 with Themis Antibody (7G7) at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 503-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26287_rabbit-themis-mab-7g7-282202412493611.jpg)
![Flow Cytometry: Themis Antibody (7G7) [NBP3-26287] - Overlay Peak curve showing Jurkat cells stained with Themis Antibody (7G7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26287_rabbit-themis-mab-7g7-282202414312728.jpg)
![Western Blot: Themis Antibody (SR2184) [NBP3-22411] - Western blot analysis using NBP3-22411 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22411_rabbit-themis-mab-sr2184-57202316582115.jpg "Western Blot: Themis Antibody (SR2184) [NBP3-22411] - Western blot analysis using NBP3-22411 in Jurkat cell lysate.")
![Western Blot: THEMIS Antibody [NBP2-27492] - (0.3 ug/ml) Analysis of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/THEMIS-Antibody-Western-Blot-NBP2-27492-img0001.jpg "Western Blot: THEMIS Antibody [NBP2-27492] - (0.3 ug/ml) Analysis of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Themis Antibody [NBP3-23438] - Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (lanes 4 & 8).](http://images.novusbio.com/fullsize/nbp3-23438_rabbit-themis-pab-1192023166531.jpg "Western Blot: Themis Antibody [NBP3-23438] - Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (lanes 4 & 8).")
![Immunoprecipitation:Themis AntibodyNBP3-29616] -Detection of human THEMIS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THEMIS antibody NBP3-29616 (lot NBP3-29616-1) used for IP at 6 µg per reaction. THEMIS was also immunoprecipitated by rabbit anti-THEMIS antibody BL20901. For blotting immunoprecipitated THEMIS, NBP3-29616 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29616_rabbit-themis-pab-301220241229391.jpg "Immunoprecipitation:Themis AntibodyNBP3-29616] -Detection of human THEMIS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THEMIS antibody NBP3-29616 (lot NBP3-29616-1) used for IP at 6 µg per reaction. THEMIS was also immunoprecipitated by rabbit anti-THEMIS antibody BL20901. For blotting immunoprecipitated THEMIS, NBP3-29616 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: Themis Antibody [NBP2-38183] - Staining of human thymus shows strong cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/Themis-Antibody-Immunohistochemistry-Paraffin-NBP2-38183-img0002.jpg "Immunohistochemistry-Paraffin: Themis Antibody [NBP2-38183] - Staining of human thymus shows strong cytoplasmic positivity in cortical cells.")
![Western Blot: THEMIS Overexpression Lysate (Adult Normal) [NBP2-10157] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for THEMIS.](http://images.novusbio.com/fullsize/THEMIS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10157-img0001.jpg "Western Blot: THEMIS Overexpression Lysate (Adult Normal) [NBP2-10157] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for THEMIS.")
