Activin A: Novus Biologicals

338-AC

Recombinant Human/Mouse/Rat Activin A Protein

(594 Publications)

10 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	338-AC-010/CF
Carrier Free	338-AC-050/CF
Carrier Free	338-AC-01M/CF
Carrier Free	338-AC-500/CF

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DAC00B

Human/Mouse/Rat Activin A Quantikine ELISA Kit

(69 Publications)

1 Kit

DAC00B

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

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AF338

Activin A betaA subunit Antibody [Unconjugated] - beta A subunit

(36 Publications)

100 μg

AF338

NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Activin A was detected in immersion fixed SK-BR-3 human breast cancer cell line (left panel, positive stain) and HeLa human cervical epithelial carcinoma cell line (right panel, negative stain) using Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Activin A was detected in immersion fixed SK-BR-3 human breast cancer cell line (left panel, positive stain) and HeLa human cervical epithelial carcinoma cell line (right panel, negative stain) using Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Activin A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human/Mouse/Rat Activin A beta A Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for <a class=NoLineLink href=

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338). The ND₅₀ is typically 2-6 µg/mL." class="big_lightbox">

Recombinant Human/Mouse/Rat Activin A (Catalog # <a class=

338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338). The ND₅₀ is typically 2-6 µg/mL." class="big_thumb" />

Native gel analysis of type II receptors and ActA, ActAC, and ActC.Native PAGE analysis of ActRIIA (A) and ActRIIB (B) with activin A (ActA) and activin AC (ActAC). Binary complexes were formed by titrating receptor from 1:1 to 3:1 molar ratio against constant ligand. (C) Native PAGE analysis of 1:3 receptor: ligand molar ratio with activin C (ActC). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35736809), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF338U-100UG
Alexa Fluor 405	AF338V-100UG
Alexa Fluor 488	AF338G-100UG
Alexa Fluor 532	AF338X-100UG
Alexa Fluor 594	AF338T-100UG
Alexa Fluor 647	AF338R-100UG
Alexa Fluor 700	AF338N-100UG
Alexa Fluor 750	AF338S-100UG
Alexa Fluor Plus 405	AF338AFP405-100UG
Alexa Fluor Plus 488	AF338AFP488-100UG
Alexa Fluor Plus 555	AF338AFP555-100UG
Alexa Fluor Plus 594	AF338AFP594-100UG
Alexa Fluor Plus 647	AF338AFP647-100UG
Alexa Fluor Plus 680	AF338AFP680-100UG
Allophycocyanin	AF338A
Biotin	BAF338
CoraFluor 1	AF338CL1
DyLight 350	AF338D
DyLight 405	AF338E
DyLight 488	AF338K
DyLight 550	AF338L
DyLight 594	AF338M
DyLight 650	AF338W
DyLight 680	AF338Y
DyLight 755	AF338Z
FITC	AF338F
HRP	AF338H
Janelia Fluor 525	AF338JF525
Janelia Fluor 549	AF338I
Janelia Fluor 646	AF338J
Janelia Fluor 669	AF338JF669
PE	AF338P
PerCP	AF338C
mFluor Violet 450 SE	AF338MFV450
mFluor Violet 500 SE	AF338MFV500
mFluor Violet 610 SE	AF338MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF338-SP

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MAB3381

Activin A betaA subunit Antibody (69403) - beta A subunit

(29 Publications)

100 μg

MAB3381

338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_lightbox">

338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND₅₀ is typically 0.02-0.06 µg/mL." alt="Recombinant Human/Mouse/ Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_thumb" />

$Activin A levels are significantly higher in tumor tissue and correlate to worse prognosis in PDAC. (A) Representative cores of pancreatic tumors from a human pancreatic tissue microarray stained with H&E to assess the percentage of epithelial versus stromal cells in each core. Adjacent sections were immunostained for activin A expression. (B) Quantification of the percentage of epithelial cells (left panel) or stromal cells (right panel) in normal tissue (white bar) versus tumor tissue (black bar). Statistical analysis is unpaired t-test. (C) Quantification of activin A in TMA (n = 63). An average of the percentage of epithelial and stromal fraction was calculated and scored in each TMA. Comparing epithelial (left panel) and stromal fraction (right panel) in normal tissue (white bar) versus tumor tissue (black bar). Statistical analysis 2-way ANOVA; mean ± SEM, ***(p < 0.001). (D) Kaplan–Meier plots of patient overall survival versus expression of activin A in either epithelial cells (left panel) or stromal cells (right panel). Statistical analysis Log-rank (Mantel-Cox) test, ** (p < 0.01), ***(p < 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33846512), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Inhibition of activin A in KPC mice significantly reduces metastasis. (A) Time course of anti-activin A neutralizing antibody injected in mice weekly (2 mg/kg). Control animals were injected with the same concentration of IgG isotype. Remaining animals were collected at the end of week 13 of treatment. (B) Representative image (×4 magnification) of H&E staining of pancreas from control treated (IgG) and anti-activin A treated KPC mice IHC-stained for either alpha -SMA (to indicate stromal cells) or activin A. Representative stromal cells marked by arrows. (C) Representative image (×4 magnification) of H&E staining of pancreas used for quantification of CN (yellow arrow) cystic lesions. Quantification of lesions is documented in both IgG control and anti-activin A antibody treated mice. (D) Left panel: Representative images (×10 and ×40 magnification) of liver tissue from control treated versus anti-activin A treated KPC mice stained with H&E (upper panel) or ductal marker protein CK19 (Lower panel). Right panel: Quantification of distant metastatic formation in the liver tissue of control (n = 5) and treated (n = 7) mice. Statistical analysis unpaired Student t-test (ns: not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33846512), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Specific activin A inhibition attenuates TGF beta 1-induced Smad3 activation and profibrotic responses in MC. a ELISA demonstrates that TGF beta 1 (24 h) increases actA and actB secretion (n = 3) to 19.5 ng/ml and 2.5 ng/ml, representing an 8.9- and 1.06-fold induction respectively. b TGF beta 1 increases actA in whole cell lysate by 1.8-fold (n = 3). c ActA (20 ng/ml) upregulates FN (n = 3–4), CTGF (n = 4) and alpha SMA (n = 6) at 48 h. d TGF beta 1 and actA both increase Smad3 transcriptional activity; no synergistic effect is seen (n = 6–12). e An actA neutralizing antibody attenuates TGF beta 1-induced FN (n = 5–6), alpha SMA (n = 5), CTGF (n = 5–6), and Smad3 activation (n = 10–12). f ActA neutralization decreases TGF beta 1-induced Smad3 transcriptional activity at 24 h (n = 9–15), but this is not decreased by actB neutralization (n = 6) (g). h MC were stimulated with TGF beta 1 or actA for 1 h, then treated with their type I receptor inhibitor SB431542 (50 µM). Restimulation with the same ligand shows that cells become refractory to TGF beta 1, but not actA (n = 4). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Activin A neutralization inhibits renal fibrosis in TGF beta 1-overexpressing mice. a TGF beta 1 transcript is increased in mice genetically engineered to overexpress TGF beta 1 (HH) compared with wild-type mice (WT) (n = 6–7, *p ≤ 0.05). b Serum actA is elevated in wild-type and HH mice after UUO. This is decreased by treatment with a neutralizing actA antibody (anti-actA) in HH mice. c Renal actA is increased after UUO, with a greater induction in HH mice. Both are attenuated by actA neutralization. Boxed areas are shown at higher magnification immediately below. ActA increases are seen particularly in tubular epithelial cells. d Renal alpha SMA, fibronectin (FN), pSmad3 and MRTF-A are increased after UUO and this is augmented in HH kidneys. Expression of all is attenuated by actA neutralization in both WT and HH kidneys. (n = 6–9) *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test where there are > 2 groups; t-test for 2 groups Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activin inhibition attenuates TGF beta 1-induced fibrotic responses and Smad3 activation in MC. Activin inhibition with follistatin (FST) decreases TGF beta 1-induced: a FN, alpha SMA and CTGF upregulation at 48 h (n = 5), b Smad3 phosphorylation (pSmad3) at 24 h (n = 5), c Smad3 nuclear translocation as assessed using eGFP-Smad3 (n = 3; 25–30 cells quantified per treatment group) at 24 h, and d Smad3 transcriptional activity at 24 h (n = 8). e Time course experiments show increases in pSmad3 occur earlier (30–60 min) with TGF beta 1 (n = 4) compared with actA (n = 4) or actB (n = 3) (18–48 h). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by R&D Systems.

DY990). Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human Activin A was serially diluted and captured by Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381) coated on a Clear Polystyrene Microplate (Catalog # <a class=

DY990). Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB3381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Activin A ?A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human Total Legumain/Asparaginyl Endopeptidase was serially diluted and captured by Mouse Anti-Human/Mouse/Rat Activin A ?A subunit Monoclonal Antibody (Catalog # <a class=

MAB3381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Activin A ?A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB3381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human Total Legumain/Asparaginyl Endopeptidase was serially diluted and captured by Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # <a class=

MAB3381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3381U-100UG
Alexa Fluor 405	FAB3381V-100UG
Alexa Fluor 488	FAB3381G-100UG
Alexa Fluor 532	FAB3381X-100UG
Alexa Fluor 594	FAB3381T-100UG
Alexa Fluor 647	FAB3381R-100UG
Alexa Fluor 700	FAB3381N-100UG
Alexa Fluor 750	FAB3381S-100UG
Alexa Fluor Plus 405	FAB3381AFP405-100UG
Alexa Fluor Plus 488	FAB3381AFP488-100UG
Alexa Fluor Plus 555	FAB3381AFP555-100UG
Alexa Fluor Plus 594	FAB3381AFP594-100UG
Alexa Fluor Plus 647	FAB3381AFP647-100UG
Alexa Fluor Plus 680	FAB3381AFP680-100UG
Allophycocyanin	FAB3381A
Biotin	BAM3381
CoraFluor 1	FAB3381CL1
DyLight 350	FAB3381D
DyLight 405	FAB3381E
DyLight 488	FAB3381K
DyLight 550	FAB3381L
DyLight 594	FAB3381M
DyLight 650	FAB3381W
DyLight 680	FAB3381Y
DyLight 755	FAB3381Z
FITC	FAB3381F
HRP	FAB3381H
Janelia Fluor 525	FAB3381JF525
Janelia Fluor 549	FAB3381I
Janelia Fluor 646	FAB3381J
Janelia Fluor 669	FAB3381JF669
PE	FAB3381P
PerCP	FAB3381C
mFluor Violet 450 SE	FAB3381MFV450
mFluor Violet 500 SE	FAB3381MFV500
mFluor Violet 610 SE	FAB3381MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3381-SP
Carrier Free	MAB3381-500

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NBP1-30928

Activin A Antibody - BSA Free

(6 Publications)

100 ul

NBP1-30928

Immunohistochemistry-Paraffin: Activin A Antibody [NBP1-30928] - CLEAR CELL OVCA xenograft, using Inhibin beta A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

<b>Biological Strategies Validation. </b>Activin-A-Antibody-Western-Blot-NBP1-30928-img0007.jpg

Western Blot: Activin A Antibody [NBP1-30928] - miR-218 directly targets INHBB & YY1, & increases INHBA expression. a Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231 transfected with miR-218 or control miRNA mimic at 24 h & 72 h, respectively. ***P < 0.001. b Western blot analyses of inhibin beta B & inhibin beta A in mimic-transfected MDA-231 cells at 48 h. c Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231-bone transfected with anti-miR-218 or control antagomiR at 24 h & 48 h, respectively.. **P < 0.01; ***P < 0.001. d Sequence alignment of miR-218 & its predicted targets INHBB & YY1. e psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control were transfected into indicated cell lines. Renilla & firefly luciferase activity was measured at 48 h. *P < 0.05; n.s. not significant. f Relative RNA level of YY1 normalized to 18S in mimic-transfected MDA-231 cells at 24 h. ***P < 0.001. g Western blot analyses of YY1 & inhibin beta A in MDA-231 cells co-transfected with miR-218 mimic & YY1-overexpressing plasmid, or the corresponding controls at 72 h. h psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control vector were transfected into MDA-231 cells. Renilla & firefly luciferase activity was measured at 48 h. **P < 0.01; n.s. not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] - miR-218-regulated inhibin beta A affects SMAD2/3 signaling in a cell-dependent manner. a & b Western blot analyses of phospho-SMAD2/3 in MDA-231 (a) or MC3T3 (b) that was serum-starved overnight & treated with EV-depleted CM collected from indicated cells for 30 min or 2 h. CM producing cell lines were transfected, PBS washed 48 h after transfection & then incubated with serum-free medium overnight before CM collection & EV depletion by ultracentrifugation. c Western blot analyses of inhibin beta A, inhibin beta B, & inhibin alpha in CM from indicated cell lines that was concentrated with medium concentrator columns. Arrows indicate the position of antigen. Control mimic-transfected MDA-231 whole cell lysate (WCL) was used as positive control for inhibin beta A & inhibin beta B. d Western blot analyses of inhibin beta A monomer or dimer (activin A) in CM from indicated cell lines under non-reducing condition & without boiling the samples. Bottom & top arrows indicate inhibin beta A monomer & dimer, respectively. e Western blot analyses of inhibin alpha in MC3T3 CM concentrated from 20 ml CM. WCL of MCF10A was used as a positive control. f & g Western blot analyses of phospho-SMAD2/3 in MDA-231 (f) or MC3T3 (g) that was serum-starved overnight & treated with CM collected from indicated cells for 30 min or 2 h. For MDA-231 cells (f), recombinant inhibin alpha protein was added to CM as indicated. For MC3T3 cells (g), anti-inhibin alpha antibody was added to CM as indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] - PC-3 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Fe, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Fe, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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DY338

Human Activin A DuoSet ELISA, 15 Plate

(17 Publications)

1 Kit (for 15 Plates)

DY338

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-43663

Inhibin beta A Antibody (CL15357) - BSA Free

100 ul

NBP3-43663

<b>Orthogonal Strategies Validation. </b>Staining of human prostate shows no positivity in glandular cells as expected.

Staining of rat cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in the upper layers.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in a subset of neurons.

Staining of human kidney shows no positivity in cells in tubules as expected.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

MOUSE Monoclonal

Host: MOUSE Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44161AF350
Alexa Fluor 405	NBP3-44161AF405
Alexa Fluor 488	NBP3-44161AF488
Alexa Fluor 532	NBP3-44161AF532
Alexa Fluor 594	NBP3-44161AF594
Alexa Fluor 647	NBP3-44161AF647
Alexa Fluor 700	NBP3-44161AF700
Alexa Fluor 750	NBP3-44161AF750
Alexa Fluor Plus 405	NBP3-44161AFP405
Alexa Fluor Plus 488	NBP3-44161AFP488
Alexa Fluor Plus 555	NBP3-44161AFP555
Alexa Fluor Plus 594	NBP3-44161AFP594
Alexa Fluor Plus 647	NBP3-44161AFP647
Alexa Fluor Plus 680	NBP3-44161AFP680
Allophycocyanin	NBP3-44161APC
Biotin	NBP3-44161B
CoraFluor 1	NBP3-44161CL1
DyLight 350	NBP3-44161UV
DyLight 405	NBP3-44161V
DyLight 488	NBP3-44161G
DyLight 550	NBP3-44161R
DyLight 594	NBP3-44161DL594
DyLight 650	NBP3-44161C
DyLight 680	NBP3-44161FR
DyLight 755	NBP3-44161IR
FITC	NBP3-44161F
HRP	NBP3-44161H
Janelia Fluor 525	NBP3-44161JF525
Janelia Fluor 549	NBP3-44161JF549
Janelia Fluor 646	NBP3-44161JF646
Janelia Fluor 669	NBP3-44161JF669
PE	NBP3-44161PE
PerCP	NBP3-44161PCP
mFluor Violet 450 SE	NBP3-44161MFV450
mFluor Violet 500 SE	NBP3-44161MFV500
mFluor Violet 610 SE	NBP3-44161MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-43663-25ul
Azide and BSA Free	NBP3-44161

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11348-AC

Recombinant Human Activin A (CHO derived) Protein, CF

(1 Publication)

100 μg

11348-AC

Recombinant Human Activin A Protein (CHO derived) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED<sub>50</sub> for this effect is 0.200-1.60 ng/mL.

2 μg/lane of Recombinant Human Activin A (CHO derived) Protein (Catalog # 11348-AC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13 and 24 kDa respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	11348-AC-020
Carrier Free	11348-AC-050
Carrier Free	11348-AC-500
Carrier Free	11348-AC-01M

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BT-ACTAH

Recombinant Human Activin A Hyperactive Protein, CF

100 μg

BT-ACTAH

Human Activin A Hyperactive Protein (Catalog # BT-ACTAH) induces SBE (SMAD-binding element) reporter activity in HEK293 human embryonic kidney cells. The hyperactive Activin A protein has greater bioactivity than Wild Type Activin A.

2 μg/lane of Recombinant Human Activin A Hyperactive Protein (Catalog # BT-ACTAH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12-14 kDa and 20-30 kDa, respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BT-ACTAH-010
Carrier Free	BT-ACTAH-050
Carrier Free	BT-ACTAH-500
Carrier Free	BT-ACTAH-01M

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Qk005

Recombinant Human/Mouse Activin A PLUS, Animal-Free Protein

50 μg

Qk005

Activin A PLUS activity is determined using an activin-responsive firefly luciferase reporter in HEK293T cells. in triplicate) with a serial dilution of activin A for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.EC<sub>50</sub> = 0.103 ng/ml (4.24 pM).

Activin A PLUS migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Reactivity:

Hu, Mu, Rt, Po, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	Qk005-0500

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11344-AC

Recombinant Human Activin A (HEK293 derived) Protein, CF

100 μg

11344-AC

Recombinant Human Activin A (HEK293 derived) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED<sub>50</sub> for this effect is 0.200-1.60 ng/mL.

2 μg/lane of Recombinant Human Activin A (HEK293 derived) Protein (Catalog # 11344-AC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14 kDa and 24 kDa, respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	11344-AC-020
Carrier Free	11344-AC-050
Carrier Free	11344-AC-500
Carrier Free	11344-AC-01M

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Qk035

Recombinant Human follistatin-resistant Activin A,AF Protein

50 μg

Qk035

Activin A protein activity is determined using an activin-responsive firefly luciferase reporter in HEK293T cells. EC<sub>50</sub> for wild-type activin A (Qk001) = 0.228 ng/ml (8.776 pM), EC50 for follistatin-resistant activin A (FRACTA, Qk035) = 0.212 ng/ml (8.2 pM).

FRACTA migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (1 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	Qk035-0500

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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