Products Related to Gene-ID: 3606

BT-018-GMP) Animal-Free (Catalog # BT-018-AFL) and RUO (BT-018) grades of Recombinant Human IL‑18/IL‑1F4 as measured in a IFN-gamma secretion assay using KG‑1 human acute myelogenous leukemia cells (orange, green, red, respectively)." class="big_lightbox"> BT-018-GMP) Animal-Free (Catalog # BT-018-AFL) and RUO (BT-018) grades of Recombinant Human IL‑18/IL‑1F4 as measured in a IFN-gamma secretion assay using KG‑1 human acute myelogenous leukemia cells (orange, green, red, respectively)." alt="Equivalent bioactivity of GMP (BT-018-GMP) Animal-Free (Catalog # BT-018-AFL) and RUO (BT-018) grades of Recombinant Human IL‑18/IL‑1F4 as measured in a IFN-gamma secretion assay using KG‑1 human acute myelogenous leukemia cells (orange, green, red, respectively)." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to T cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to T cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-18/IL-1F4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91245) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to T cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." class="big_lightbox"> 210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." alt="In the presence of Recombinant Human TNF-alpha (Catalog # 210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB29241) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-18/IL-18 BPa complex protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

11236-BP) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-18/IL-1F4 (Catalog # BT9124) that produces 50% of the binding response is 1.50-12.0 ng/mL." class="big_lightbox"> 11236-BP) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-18/IL-1F4 (Catalog # BT9124) that produces 50% of the binding response is 1.50-12.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 BPa His-tag Protein (11236-BP) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-18/IL-1F4 (Catalog # BT9124) that produces 50% of the binding response is 1.50-12.0 ng/mL." class="big_thumb" />

210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_lightbox"> 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." alt="In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB9124). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_thumb" />

210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant HumanIL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91241). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox"> 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant HumanIL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91241). The ND₅₀ is typically 3-15 µg/mL." alt="In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant HumanIL-18/IL-1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91241). The ND₅₀ is typically 3-15 µg/mL." class="big_thumb" />