DY990). Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog #
MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
DY990). Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog #
MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human IL-18/IL-1F4 protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91243) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog #
MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
DY990). Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-18/IL-1F4 protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![N/A IL-18/IL-1F4 [Biotin]](http://images.novusbio.com/fullsize/dy29241-05_canine-il-18-duoset-elisa-5-plate-2062024135930..png "N/A IL-18/IL-1F4 [Biotin]")
![Western Blot: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution.<br/><br/> Lane 1: A431 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 22 kDa<br/> Observed band size: 22 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32468) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32468_rabbit-il-18-il-1f4-mab-ps00-41-278202416285231.jpg "Western Blot: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution.<br/><br/> Lane 1: A431 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 22 kDa<br/> Observed band size: 22 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32468) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32468) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32468_rabbit-il-18-il-1f4-mab-ps00-41-29820241358551.jpg)
![Western blot analysis on different lysates with NBP3-32468 at 1/1,000 dilution. Lane 1: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 20 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32468 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32468_-western-blot-639204595236056480.jpg)
![ELISA: Rat IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP2-76579] - Samples were spiked with high concentrations of Rat IL-18/IL-1F4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76579_rat-il-18-il-1f4-elisa-kit-colorimetric-132202416235790.jpg "ELISA: Rat IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP2-76579] - Samples were spiked with high concentrations of Rat IL-18/IL-1F4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP2-76579] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76579_rat-il-18-il-1f4-elisa-kit-colorimetric-432024859786.jpg)
![Western Blot: IL-18/IL-1F4 Antibody (PS01-06) [NBP3-32469] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32469) at 1/1,000 dilution.<br/><br/> Lane 1: A431 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 22 kDa<br/> Observed band size: 22 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32469) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32469_rabbit-il-18-il-1f4-mab-ps01-06-278202415295548.jpg "Western Blot: IL-18/IL-1F4 Antibody (PS01-06) [NBP3-32469] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32469) at 1/1,000 dilution.<br/><br/> Lane 1: A431 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 22 kDa<br/> Observed band size: 22 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32469) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: IL-18/IL-1F4 Antibody (PS01-06) [NBP3-32469] - Flow cytometric analysis of A431 cells labeling IL-18/IL-1F4.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32469, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32469_rabbit-il-18-il-1f4-mab-ps01-06-29202416453270.jpg)
![Western blot analysis on different lysates with NBP3-32469 at 1/5,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32469, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/nbp3-32469_-western-blot-639204595236133064.jpg)
![Immunohistochemistry: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32451) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32451_rabbit-il-18-il-1f4-mab-psh02-08-298202414161830.jpg "Immunohistochemistry: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32451) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32451 at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32451 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32451_-western-blot-639204595248428260.jpg)
![Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.](http://images.novusbio.com/fullsize/IL-18-IL-1F4-Antibody-125-2H-Western-Blot-NBP2-81040-img0001.jpg "Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.")
![Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody (S02-8B2) [NBP3-20042] - Immunohistochemistry of IL-18/IL-1F4 in paraffin-embedded Human colon cancer tissue using NBP3-20042 at dilution 1/50](http://images.novusbio.com/fullsize/IL-18-IL-1F4-Antibody-S02-8B2-Immunohistochemistry-Paraffin-NBP3-20042-img0001.jpg "Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody (S02-8B2) [NBP3-20042] - Immunohistochemistry of IL-18/IL-1F4 in paraffin-embedded Human colon cancer tissue using NBP3-20042 at dilution 1/50")
![Western Blot: IL-18/IL-1F4 Antibody (S02-8B2) [NBP3-20042] - Western blot detection of IL-18/IL-1F4 in Hela cell lysates using NBP3-20042 (1:1000 diluted). Predicted band size: 22kDa. Observed band size: 17kDa.](http://images.novusbio.com/fullsize/nbp3-20042_rabbit-il-18-il-1f4-mab-s02-8b2-23420241545353.jpg)
![Western Blot: IL18 Overexpression Lysate (Adult Normal) [NBP2-04199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL18.](http://images.novusbio.com/fullsize/IL18-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04199-img0001.jpg "Western Blot: IL18 Overexpression Lysate (Adult Normal) [NBP2-04199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL18.")
