| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48106 | |||
| Azide and BSA Free | NBP3-11518 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47722-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48006-0.1mg | |||
| IHC-Prediluted | NBP2-48105 | |||
![Western Blot: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Western blot analysis of HLA-DR on different lysates with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HUT 102 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32435) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-278202416331238.jpg "Western Blot: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Western blot analysis of HLA-DR on different lysates with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HUT 102 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32435) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414262655.jpg)
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32435, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414161847.jpg)
![Immunocytochemistry/ Immunofluorescence: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunocytochemistry analysis of Daudi cells labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-2920241192357.jpg)
![Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-4Y9Y8-Western-Blot-NBP3-15337-img0002.jpg "Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Human spleen using HLA-DR Rabbit mAb (NBP3-15337) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-4Y9Y8-Immunohistochemistry-Paraffin-NBP3-15337-img0001.jpg)
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human tonsil tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-5220251718983.jpg)
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human liver tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-52202517251958.jpg)
![Immunohistochemistry-Paraffin: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044] - Human Tonsil stained with HLA-DRA Monoclonal Antibody (19-26.1).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-19-26-1-same-as-MB-26-1-Immunohistochemistry-Paraffin-NBP2-45044-img0001.jpg "Immunohistochemistry-Paraffin: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044] - Human Tonsil stained with HLA-DRA Monoclonal Antibody (19-26.1).")
![Flow Cytometry: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044] - Flow Cytometric Analysis of Raji cells. HLA DRA Antibody (19-26.1 (same as MB-26.1)) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-19-26-1-same-as-MB-26-1-Flow-Cytometry-NBP2-45044-img0003.jpg)
![Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).](http://images.novusbio.com/fullsize/p_2392_1.jpg "Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).")
![Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-169-1B5-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47722-img0003.jpg "Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-169-1B5-2-Flow-Cytometry-NBP2-45043-img0004.jpg "Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) [NBP2-44985] - Human Tonsil stained with HLA-DP/DR Monoclonal Antibody (Bra-14).](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-Bra-14-Immunohistochemistry-Paraffin-NBP2-44985-img0001.jpg "Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) [NBP2-44985] - Human Tonsil stained with HLA-DP/DR Monoclonal Antibody (Bra-14).")
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (HL-40) [NB500-408] - Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR/DP (HL-40).](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-HL-40-Immunohistochemistry-Paraffin-NB500-408-img0001.jpg "Immunohistochemistry-Paraffin: HLA DR/DP Antibody (HL-40) [NB500-408] - Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR/DP (HL-40).")
![Western Blot: HLA DRA Antibody [NBP1-31512] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Western-Blot-NBP1-31512-img0002.jpg "Western Blot: HLA DRA Antibody [NBP1-31512] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-31512] - Paraffin-embedded Leukocytes in liver carcinoma. HLA-DRA antibody dilution: 1:250.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-31512-img0003.jpg)