| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99941-50ul | |||
HAF019). A specific band was detected for Glyoxalase II at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glyoxalase II at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5944-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38909-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90061-50ul | |||
![Western Blot: Glyoxalase II/HAGH Antibody [NBP2-99941] - Anti-Glyoxalase II/HAGH rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP2-99941-img0006.jpg "Western Blot: Glyoxalase II/HAGH Antibody [NBP2-99941] - Anti-Glyoxalase II/HAGH rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa")
![Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP2-99941] - Immunochemical staining of human Glyoxalase II/HAGH in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunohistochemistry-Paraffin-NBP2-99941-img0004.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP2-99941] - Immunochemical staining of human Glyoxalase II/HAGH in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunohistochemistry-Paraffin-NBP2-99941-img0003.jpg)
![Immunoprecipitation: Glyoxalase II/HAGH Antibody [NBP2-99941] - Glyoxalase II/HAGH was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Glyoxalase II/HAGH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Glyoxalase II/HAGH rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunoprecipitation-NBP2-99941-img0005.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP1-33764] - Mouse kidney. HAGH antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunohistochemistry-Paraffin-NBP1-33764-img0008.jpg "Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP1-33764] - Mouse kidney. HAGH antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: Glyoxalase II/HAGH Antibody [NBP1-33764] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP1-33764-img0009.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP1-33764] - NCI-N87 xenograft, using HAGH antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunohistochemistry-Paraffin-NBP1-33764-img0005.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (NBP1-33764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33764_rabbit-polyclonal-glyoxalase-ii-hagh-antibody-western-blot-2611202518274623.jpg)
![Western Blot: Glyoxalase II/HAGH Antibody [NBP2-16778] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP2-16778-img0003.jpg "Western Blot: Glyoxalase II/HAGH Antibody [NBP2-16778] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: HAGH Antibody [NBP2-16778] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HAGH-Antibody-Immunohistochemistry-Paraffin-NBP2-16778-img0002.jpg)
![Western Blot: Glyoxalase II/HAGH Antibody [NBP2-38909] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP2-38909-img0002.jpg "Western Blot: Glyoxalase II/HAGH Antibody [NBP2-38909] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue")
![Immunohistochemistry-Paraffin: Glyoxalase II/HAGH Antibody [NBP2-38909] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Immunohistochemistry-NBP2-38909-img0001.jpg)
![Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56760] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP1-56760-img0003.jpg "Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56760] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.")
![Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56760] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP1-56760-img0002.jpg)
![Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56760] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP1-56760-img0004.jpg)
![Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56674] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Glyoxalase-II-HAGH-Antibody-Western-Blot-NBP1-56674-img0003.jpg "Western Blot: Glyoxalase II/HAGH Antibody [NBP1-56674] - Human Liver cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HAGH Overexpression Lysate (Adult Normal) [NBL1-11438] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HAGH.](http://images.novusbio.com/fullsize/HAGH-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11438-img0002.jpg "Western Blot: HAGH Overexpression Lysate (Adult Normal) [NBL1-11438] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HAGH.")
