| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22423-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8508-SP | |||
| Carrier Free | IC8508N-100UG | |||
| Carrier Free | IC8508G-100UG | |||
| Carrier Free | IC8508V-100UG | |||
| Carrier Free | IC8508U-100UG | |||
| Carrier Free | IC8508T-100UG | |||
| Carrier Free | IC8508S-100UG | |||
| Carrier Free | IC8508R-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8508-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86144-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03734-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85798-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB85081-SP | |||
![Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Western-Blot-NBP2-22423-img0003.jpg "Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-31447] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31447-img0003.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-31447] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31447-img0004.jpg)
![Western Blot: GIT1 Antibody [NBP1-31447] - Rat2 whole cell lysate/extract.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-31447-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31447_rabbit-polyclonal-git1-antibody-western-blot-141120251826021.jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31447_rabbit-polyclonal-git1-antibody-western-blot-1411202518312121.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-86144] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86144-img0006.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-86144] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.")
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0009.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human kidney shows strong cytoplasmic positivity in glomeruli.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0007.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03734-img0003.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![ICC/IF-GIT1 Antibody [NBP3-03734] - Analysis of L929 cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GIT1-Antibody-Binding-Activity-NBP3-03734-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734] - Immunofluorescence analysis of C6 cells using [KO Validated] GIT1 Rabbit pAb (A15437) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03734_rabbit-polyclonal-git1-antibody-52202519313438.jpg)
![Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts of various cell lines, using GIT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GIT1-Antibody-Knockout-Validated-NBP3-03734-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/GIT1-Antibody-Knockout-Validated-NBP3-03734-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-85798] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85798-img0006.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-85798] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.")
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85798-img0007.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-85798_rabbit-polyclonal-git1-antibody-24420259374136.jpg)
![Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-19141-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - Human breast carcinoma. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-19141-img0003.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-19141-img0001.jpg)
![N/A GIT1 [Biotin]](http://images.novusbio.com/fullsize2/GIT1_DY848505_ELISA_2574.jpg "N/A GIT1 [Biotin]")
![Western Blot: GIT1 Overexpression Lysate (Adult Normal) [NBP2-07386] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GIT1.](http://images.novusbio.com/fullsize/GIT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07386-img0001.jpg "Western Blot: GIT1 Overexpression Lysate (Adult Normal) [NBP2-07386] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GIT1.")