| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06398SS | |||
HAF017). A specific band was detected for Glutathione Peroxidase 3 at approximately 24kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human plasma, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3 at approximately 24kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03845-20ul | |||
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - Analysis of GPX3 in human kidney lysate.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Western-Blot-NBP1-06398-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 1:200 conc. of Glutathione Peroxidase 3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Glutathione Peroxidase 3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-Paraffin-NBP1-06398-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - The GPX3 antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI blue).](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06398-img0003.jpg)
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - ALDH1A3 & GPX3 expressions in squamous carcinomas/adenosquamous carcinomas (SC/ASC). EnVision immunohistochemistry, original magnification ×200. ALDH1A3 & GPX3 positive reaction was mainly localized in the cytoplasm. (a), positive ALDH1A3 expression in poorly differentiated SC/ASC. (b), negative ALDH1A3 expression in well-differentiated SC/ASC. (c), negative GPX3 expression in poorly differentiated SC/ASC. (d), positive GPX3 expression in well-differentiated SC/ASC. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24167362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06398_rabbit-polyclonal-glutathione-peroxidase-3-gpx3-antibody-31020241517522.jpg)
![Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - ALDH1A3 & GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification ×200. ALDH1A3 & GPX3 positive reaction was mainly localized in the cytoplasm. (a), positive ALDH1A3 expression in moderately differentiated AC. (b), negative ALDH1A3 expression in well-differentiated AC. (c), negative GPX3 expression in poorly differentiated AC. (d), positive GPX3 expression in well-differentiated AC. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24167362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06398_rabbit-polyclonal-glutathione-peroxidase-3-gpx3-antibody-310202415291925.jpg)
CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in tubules. View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![SDS-Page: Recombinant Human Glutathione Peroxidase 3/GPX3 Protein [NBP1-99090]](http://images.novusbio.com/fullsize/Recombinant-Human-Glutathione-Peroxidase-3-GPX3-Protein-SDS-Page-NBP1-99090-img0002.jpg "SDS-Page: Recombinant Human Glutathione Peroxidase 3/GPX3 Protein [NBP1-99090]")
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Western-Blot-NBP2-82233-img0002.jpg "Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - Rabbit Anti-GPX3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue. Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells. Primary Antibody Concentration: 1:100. Other Working Concentrations: 1/600. Secondary Antibod](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-Paraffin-NBP2-82233-img0001.jpg)
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody - Azide and BSA Free [Glutathione Peroxidase 3/GPX3] - Western blot analysis of lysates from Mouse kidney, using Glutathione Peroxidase 3/GPX3 Rabbit pAb at 1:800 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03845_rabbit-polyclonal-glutathione-peroxidase-3-gpx3-antibody-5220251628015.jpg "Western Blot: Glutathione Peroxidase 3/GPX3 Antibody - Azide and BSA Free [Glutathione Peroxidase 3/GPX3] - Western blot analysis of lysates from Mouse kidney, using Glutathione Peroxidase 3/GPX3 Rabbit pAb at 1:800 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.")
