| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83302-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76738-0.025mg | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PDCD4 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human PDCD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7019) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7019-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03401-20ul | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PDCD4 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human PDCD4 Monoclonal Antibody (Catalog # MAB7019) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7019-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18224-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83301-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98857-50ul | |||
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining in human breast and skeletal muscle tissues . Corresponding PDCD4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining in human breast and skeletal muscle tissues . Corresponding PDCD4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0020.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining of human breast shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0016.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0017.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining of human skin shows strong nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0018.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83302] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83302-img0019.jpg)
![Western Blot: PDCD4 Antibody [NBP1-76738] - A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1ug/ml.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Western-Blot-NBP1-76738-img0001.jpg "Western Blot: PDCD4 Antibody [NBP1-76738] - A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1ug/ml.")
![Immunohistochemistry: PDCD4 Antibody - BSA Free [NBP1-76738] - Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76738_rabbit-polyclonal-pdcd4-antibody-2711202420333811.jpg)
![Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014] - Analysis of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteasome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Western-Blot-NB110-60014-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDCD4 [p Ser457] Antibody [NB110-60014] - Analysis of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteasome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PDCD4 [p Ser457] Antibody [NB110-60014] - Used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Immunohistochemistry-NB110-60014-img0004.jpg)
NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for ![Western Blot: PDCD4 Antibody [NBP3-03401] - Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) 293T cells, using PDCD4 antibody (NBP3-03401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Western-Blot-NBP3-03401-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PDCD4 Antibody [NBP3-03401] - Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) 293T cells, using PDCD4 antibody (NBP3-03401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP3-03401] - Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PDCD4 Rabbit pAb (NBP3-03401) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP3-03401-img0003.jpg)
![Western Blot: PDCD4 Antibody [NBP3-03401] - Western blot analysis of extracts of various cell lines, using PDCD4 antibody (NBP3-03401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PDCD4 Antibody-Western Blot-NBP3-03401-img0005.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP3-03401] - Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] PDCD4 Rabbit pAb (NBP3-03401) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP3-03401-img0002.jpg)
![Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Western-Blot-NBP3-13143-img0004.jpg "Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Immunohistochemistry-Paraffin-NBP3-13143-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:1000.](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Western-Blot-NBP3-13143-img0001.jpg)
![Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PDCD4-[p-Ser457]-Antibody-Immunohistochemistry-Paraffin-NBP3-13143-img0002.jpg)
![Western Blot: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04329 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate.](http://images.novusbio.com/fullsize/PDCD4-Antibody-k4C1-BSA-Free-Western-Blot-NBP1-04329-img0003.jpg "Western Blot: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04329 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate.")
![Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP1-04329 (1:100). The secondary antibody (green) used was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/PDCD4-Antibody-k4C1-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-04329-img0002.jpg)
![Flow Cytometry: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP1-04329 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PDCD4-Antibody-k4C1-BSA-Free-Flow-Cytometry-NBP1-04329-img0004.jpg)
![Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744] - Western blot analysis of extracts of various cell lines, using PDCD4 Rabbit mAb (NBP3-16744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/PDCD4-Antibody-3A8V10-Western-Blot-NBP3-16744-img0003.jpg "Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744] - Western blot analysis of extracts of various cell lines, using PDCD4 Rabbit mAb (NBP3-16744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744] - Immunohistochemistry of paraffin-embedded human placenta using PDCD4 Rabbit mAb (NBP3-16744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDCD4-Antibody-3A8V10-Immunohistochemistry-Paraffin-NBP3-16744-img0002.jpg)
![Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744] - Immunohistochemistry of paraffin-embedded rat lung using PDCD4 Rabbit mAb (NBP3-16744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDCD4-Antibody-3A8V10-Immunohistochemistry-Paraffin-NBP3-16744-img0001.jpg)
![Western Blot: PDCD4 Antibody (4C2) [NBP3-18224] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-18224 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate](http://images.novusbio.com/fullsize/PDCD4-Antibody-4C2-Western-Blot-NBP3-18224-img0003.jpg "Western Blot: PDCD4 Antibody (4C2) [NBP3-18224] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-18224 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP3-18224 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/PDCD4-Antibody-4C2-Immunocytochemistry-Immunofluorescence-NBP3-18224-img0002.jpg)
![Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP3-18224 at 2-5ug for 1x10^6cells (red). A Goat anti-mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PDCD4-Antibody-4C2-Flow-Cytometry-NBP3-18224-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PDCD4 Antibody [NBP1-83301] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83301-img0004.jpg "Immunocytochemistry/Immunofluorescence: PDCD4 Antibody [NBP1-83301] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-83301] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PDCD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83301-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PDCD4 Antibody [NBP2-98857] - Immunofluorescence staining of PDCD4 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98857 antibody (1:1000)(Figure A), incubated with NBP2-98857 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98857_rabbit-polyclonal-pdcd4-antibody-235202512205516.png "Immunocytochemistry/Immunofluorescence: PDCD4 Antibody [NBP2-98857] - Immunofluorescence staining of PDCD4 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98857 antibody (1:1000)(Figure A), incubated with NBP2-98857 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.")
