HAF017). A specific band was detected for XPB at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, NRK rat normal kidney cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat XPB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPB at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6349-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58758-25ul | |||
![Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPB-Antibody-Western-Blot-NB100-61060-img0006.jpg "Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody NB100-61060 used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody NB100-61059. For blotting immunoprecipitated ERCC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPB-Antibody-Immunoprecipitation-NB100-61060-img0005.jpg)
![Western Blot: XPB Antibody [NB100-61060] - Effects of triptolide & histone depletion on condensation using mouse sperm.(A) Representative fluorescence images of chromatid assembly at steady state with mouse sperm nuclei in egg extracts (180 min after sperm nuclei addition) in the presence of indicated inhibitors. Triptolide or DMSO control was added at 25 min after nuclei addition. Triptolide was added at 50 μM. (B) Western blot for histone H3, CAP-E, & XPB in histone H4K12ac or IgG-depleted extracts in the presence of triptolide (TPL) or DMSO. Relative percentage of protein remaining after indicated treatments is shown.Figure 5—figure supplement 1—source data 1.Source data for Figure 5—figure supplement 1B.Source data for Figure 5—figure supplement 1B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61060_rabbit-polyclonal-xpb-antibody-31020241534338.jpg)
![Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61060_rabbit-polyclonal-xpb-antibody-31020241612617.jpg)
![Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61060_rabbit-polyclonal-xpb-antibody-31020241555238.jpg)
![Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - Detection of human ERCC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERCC3 (NB100-61059). Detection: DAB](http://images.novusbio.com/fullsize/XPB-Antibody-Immunohistochemistry-NB100-61059-img0006.jpg "Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - Detection of human ERCC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERCC3 (NB100-61059). Detection: DAB")
![Immunoprecipitation: XPB Antibody [NB100-61059] - Detection of human ERRC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERRC3 antibody NB100-61059 used for IP at 3 ug per reaction. ERRC3 was also immunoprecipitated by rabbit anti-ERRC3 antibody NB100-61060. For blotting immunoprecipitated ERRC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPB-Antibody-Immunoprecipitation-NB100-61059-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: XPB Antibody [NBP2-58758] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/XPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58758-img0001.jpg "Immunocytochemistry/Immunofluorescence: XPB Antibody [NBP2-58758] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: XPB Overexpression Lysate (Adult Normal) [NBL1-10321] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XPB.](http://images.novusbio.com/fullsize/XPB-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10321-img0002.jpg "Western Blot: XPB Overexpression Lysate (Adult Normal) [NBL1-10321] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XPB.")
![Western Blot: XPB Antibody [NBP3-10467] - Western blot analysis using NBP3-10467 on Human HCT15 as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/XPB-Antibody-Western-Blot-NBP3-10467-img0001.jpg "Western Blot: XPB Antibody [NBP3-10467] - Western blot analysis using NBP3-10467 on Human HCT15 as a positive control. Antibody Titration: 0.2-1 ug/ml")
