| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35370-20ul | |||
VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11570-SP | |||
![Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0001.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183313.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183326.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249422686.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog intestine. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393596.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-21202494084.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat tissues. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249383128.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249351354.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393541.jpg)
![Immunohistochemistry: CPT1A Antibody [NBP3-35370] - Immunohistochemistry analysis of paraffin-embedded Human colon using CPT1A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-101220241153586.jpg "Immunohistochemistry: CPT1A Antibody [NBP3-35370] - Immunohistochemistry analysis of paraffin-embedded Human colon using CPT1A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: CPT1A Antibody [NBP3-35370] - Western blot analysis of lysates from HeLa cells using CPT1A Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-1012202411552896.jpg)
![Immunocytochemistry/ Immunofluorescence: CPT1A Antibody [NBP3-35370] - Immunofluorescence analysis of Hep G2 cells using CPT1A Rabbit pAbat a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-31120251704717.jpg)
![Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP2-15993-img0004.jpg "Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - Mouse kidney. CPT1A stained by CPT1A antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0005.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - MDA-MB157 xenograft, using CPT1A antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0002.jpg)
![Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29820241435278.jpg "Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Flow cytometric analysis of HepG2 cells labeling CPT1A.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32213, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29202416453278.jpg)
![Western blot analysis on different lysates with NBP3-32213 at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32213 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32213_-western-blot-639204597484764815.jpg)
![Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0006.jpg "Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.")
![Western Blot: CPT1A Antibody [NBP1-59608] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0002.jpg)
![Western Blot: CPT1A Antibody [NBP1-59608] - Sample Tissue: 293T, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0005.jpg)
![Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-33364_rabbit-cpt1a-mab-8y1j4-91220241410180.jpg "Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from wild type (WT) and CPT1A knockdown (KD) HeLa cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-33364_rabbit-cpt1a-mab-8y1j4-52202511411482.jpg)
![Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-33364_rabbit-cpt1a-mab-8y1j4-5220251215477.jpg)
![Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Western blot analysis of lysates from MCF7 cells, using CPT1A Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16072_rabbit-cpt1a-mab-3m6e9-52202511383769.jpg "Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Western blot analysis of lysates from MCF7 cells, using CPT1A Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBP2-09778] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09778-img0001.jpg "Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBP2-09778] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.")
![Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBL1-09449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09449-img0002.jpg "Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBL1-09449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.")
