LRRK2: Novus Biologicals

NB300-268

LRRK2 Antibody - BSA Free

(1 Review)

(46 Publications)

0.1 ml

NB300-268

LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0026.jpg

<b>Independent Antibodies Validation. </b>LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0025.jpg

Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The primary antibody was used at a dilution of 1:200 and incubated for 20 min at room temperature. Image from verified customer review.

LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0024.jpg

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-LRRK2 Antibody NB300-268 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red), and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60X magnification.

Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on Neuro2a cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

<b>Biological Strategies Validation. </b>LRRK2 kinase inhibition blocks Tat-induced S935 phosphorylation and inflammatory cytokine expression in BV-2 cells. (A) Western blot depicting pS935-LRRK2 and total LRRK2 protein levels 12 hours after saline or Tat-treatment ± LRRK2 kinase inhibition (LRRK2i).

The interaction and phosphorylation of LRRK2 and LC1. (A) Cellular co-localisation of endogenous LRRK2 and MAP1B (LC1). SKNSH cells were labelled with LRRK2 (green) and LC1 (red) antibodies and signals were detected by immunofluorescence. Detailed figure in Additional file 1.

Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Western blotting of LRRK2. A. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T & M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, & Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). B. Recombinant LRRK2 (arrowhead) from transfected M17 cells is detected by anti-LRRK2 Ab4 (lane 1), but not after absorption of the antibody with its peptide antigen (lane 2). Brain LRRK2 was recognized by anti-LRRK2 Ab4 in two controls (lanes 3 & 4) & two PD cases (lanes 5 & 6). Cell lysates (10 μg protein) & brain homogenates (100 μg protein) were prepared & loaded on 6% SDS-PAGE gels for Western blot analysis using anti-LRRK2 antibodies Ab1–Ab4 in (A) & Ab4 in (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: LRRK2 Antibody - BSA Free [NB300-268] - Deletion of LRRK2 attenuates Mn-induced apoptosis & cell death in RAW 264.7 cells.(A) LRRK2 WT or KO RAW 264.7 cells were treated with Mn (250 μM) for the designated times, followed by flow cytometry analysis to determine Mn-induced apoptosis. Both early & late apoptotic cells (Q2 & Q3) were measured. (B) At the end of Mn exposure, cell viability was determined by MTT assay. @@@, p < 0.001; ###, p < 0.001; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3, for apoptosis assay; n = 6, for MTT assay). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 accumulates in globules in alpha S tg mice. (a & b) Double immunofluorescence for alpha S with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alpha S tg mice (a) & P123H beta S tg mice (b). Note that alpha S-globules were immunopositive for LRRK2 (~79%, n = 22), whereas P123H beta S globules were negative for LRRK2. Representative images are shown for the thalamus ( alpha S) & basal ganglia (P123H beta S). Scale bar = 5 μm for all panels. (c) Triple immunofluorescence for alpha S, LRRK2 & Rab5B for basal ganglia in alpha S tg mice. LRRK2 & Rab5B were colocalized in axon terminal (arrow), but were not colocalized in the alpha S-globule (arrowhead) Scale bar = 10 μm for all panels. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Specificity of immunostaining for LRRK2. Specificity of the antibodies used for immunocytochemistry was determined by performing adsorptions with the corresponding peptide sequences. Immunochemical localization of LBs (arrows) by Ab4 against LRRK22500–2527 (A) & Ab1 against LRRK2900–100 (C) was blocked using respective peptide antigens (B & D). Adjacent sections with LB within pigmented neurons from the substantia nigra of a case of PD were used. Scale bar: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 deletion attenuates Mn-induced pro-apoptotic protein levels.(A) After cells (LRRK2 WT & LRRK2 KO) were exposed to Mn (250 μM) for up to 24 h, total protein was extracted & followed by western blot analysis to determine protein levels of Bax & Daxx. beta -actin was used as a loading control. (B) The expression levels of Bax & Daxx modulated by Mn were quantified relative to its control levels in LRRK2 WT & KO cells. **, p < 0.01; ***, p < 0.001 (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - The interaction & phosphorylation of LRRK2 & LC1. (A) Cellular co-localisation of endogenous LRRK2 & MAP1B (LC1). SKNSH cells were labelled with LRRK2 (green) & LC1 (red) antibodies & signals were detected by immunofluorescence. Detailed figure in Additional file 1. (B) LRRK2 kinase assay. The LRRK2 wild-typed (WT) kinase interacted & phosphorylated the LC1 domain unlike the LRRK2 kinase-dead protein. (C) Western blotting of LRRK2 & LC1 proteins after a kinase assay. Both LRRK2 & LC1 proteins were probed for phospho-threonine & phospho-serine signals respectively. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-7-29), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in PD. Four antibodies raised against sequences corresponding to various regions shown on the schematic diagram of LRRK2 (red bars) were used on brainstem sections of PD (A-H) & age-matched controls (I-L). Intense immunolabeling of brainstem LBs in cases of PD was seen with Ab1 against LRRK2900–100 (A) & Ab4 against LRRK22500–2527(D). Both rim (A & D) & core (inset in D) of LBs were recognized. In contrast, LBs were not labelled in any case using antibodies directed against LRRK21246–1265 (Ab2, B) or LRRK21838–2133 (Ab3, C), for which the antigenic sites are located within the folded domains. The cell bodies of both pigmented & no-pigmented neurons (arrows) as well as axons (arrowheads) contain LRRK2, seen only using antibodies against sites outside the folded domains (Ab1, E & Ab4, H). In contrast, Ab4 staining was much less intense in control tissue (L). The muscle layer of both large & small vessels was consistently found to contain high levels of LRRK2 in almost all PD cases (seen in E, G, & H) & strikingly the vessels are the only structure immunolabeled with Ab3 recognizing LRRK21838–2133 in all cases studied (G & K). Ab2 to LRRK21246–1265 did not recognize LBs, cell bodies or vessels in any case (B, F, & J). Scale bars: A-D & inset = 10 μm; E-L = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 kinase inhibition blocks Tat-induced S935 phosphorylation & inflammatory cytokine expression in BV-2 cells. (A) Western blot depicting pS935-LRRK2 & total LRRK2 protein levels 12 hours after saline or Tat-treatment ± LRRK2 kinase inhibition (LRRK2i). (B) Tat treatment significantly increased pS935-LRRK2, which was attenuated by LRRK2i. (C) We measured TNF-alpha , IL-6, MCP-1 & IL-10 mRNA levels by qRT-PCR 12 hours post saline or Tat treatment ± LRRK2i. LRRK2i attenuated Tat-induced TNF-alpha , IL-6, MCP-1 & IL-10 expression for all groups (*P <0.05, ***P <0.001, one way ANOVA, Newman-Keuls post-test). ANOVA, analysis of variance; LRRK2, leucine-rich repeat kinase2; pS935, phosphorylation of serine 935; Tat, trans activator of transcription. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: LRRK2 Antibody - BSA Free [NB300-268] - Inhibition of LRRK2 kinase activity attenuates Mn-induced apoptosis.(A) After pre-treatment with GSK (1 μM) for 90 min, cells (HMC3) were exposed to Mn (250 μM) for the designated time periods, followed by annexin V & PI staining & flow cytometry analysis to determine apoptosis. Early & late apoptotic cells (Q2 & Q3) were analyzed. (B,C) After pre-treatment with GSK (1 μM) for 90 min, cells (LRRK2 WT RAW 264.7 & HMC3) were exposed to Mn for designated time periods, followed by the MTT assay to determine cell viability, as described in the Methods section, (@@@, p < 0.001; *, p < 0.05; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3, apoptosis assay; n = 6, MTT assay). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

LRRK2 was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-LRRK2 Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB300-268AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Bv, Ce, I, Pl

Species Glossary

Reactivity: Hu, Mu, Bv, Ce, I, Pl

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-268AF350
Alexa Fluor 405	NB300-268AF405
Alexa Fluor 488	NB300-268AF488
Alexa Fluor 532	NB300-268AF532
Alexa Fluor 594	NB300-268AF594
Alexa Fluor 647	NB300-268AF647
Alexa Fluor 700	NB300-268AF700
Alexa Fluor 750	NB300-268AF750
Alexa Fluor Plus 405	NB300-268AFP405
Alexa Fluor Plus 488	NB300-268AFP488
Alexa Fluor Plus 555	NB300-268AFP555
Alexa Fluor Plus 594	NB300-268AFP594
Alexa Fluor Plus 647	NB300-268AFP647
Alexa Fluor Plus 680	NB300-268AFP680
Allophycocyanin	NB300-268APC
Biotin	NB300-268B
CoraFluor 1	NB300-268CL1
DyLight 350	NB300-268UV
DyLight 405	NB300-268V
DyLight 488	NB300-268G
DyLight 550	NB300-268R
DyLight 594	NB300-268DL594
DyLight 650	NB300-268C
DyLight 680	NB300-268FR
DyLight 755	NB300-268IR
FITC	NB300-268F
HRP	NB300-268H
Janelia Fluor 525	NB300-268JF525
Janelia Fluor 549	NB300-268JF549
Janelia Fluor 646	NB300-268JF646
Janelia Fluor 669	NB300-268JF669
PE	NB300-268PE
PerCP	NB300-268PCP
mFluor Violet 450 SE	NB300-268MFV450
mFluor Violet 500 SE	NB300-268MFV500
mFluor Violet 610 SE	NB300-268MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-268SS

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NB110-58771

LRRK2 Antibody - BSA Free

(8 Publications)

0.1 ml

NB110-58771

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB110-58771] - Immunofluorescence of Lrrk2 with NB110-58771 within (A) Substantia nigra neurons (B) Chains of migrating neuroblasts.

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.

<b>Genetic Strategies Validation. </b>LRRK2-Antibody-Knockdown-Validated-NB110-58771-img0010.jpg

Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( beta III-tubulin, Tau, & alpha -synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with beta III-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha -tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF beta III transforming growth factor beta III, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Po

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-58771AF350
Alexa Fluor 405	NB110-58771AF405
Alexa Fluor 488	NB110-58771AF488
Alexa Fluor 532	NB110-58771AF532
Alexa Fluor 594	NB110-58771AF594
Alexa Fluor 647	NB110-58771AF647
Alexa Fluor 700	NB110-58771AF700
Alexa Fluor 750	NB110-58771AF750
Alexa Fluor Plus 405	NB110-58771AFP405
Alexa Fluor Plus 488	NB110-58771AFP488
Alexa Fluor Plus 555	NB110-58771AFP555
Alexa Fluor Plus 594	NB110-58771AFP594
Alexa Fluor Plus 647	NB110-58771AFP647
Alexa Fluor Plus 680	NB110-58771AFP680
Allophycocyanin	NB110-58771APC
Biotin	NB110-58771B
CoraFluor 1	NB110-58771CL1
DyLight 350	NB110-58771UV
DyLight 405	NB110-58771V
DyLight 488	NB110-58771G
DyLight 550	NB110-58771R
DyLight 594	NB110-58771DL594
DyLight 650	NB110-58771C
DyLight 680	NB110-58771FR
DyLight 755	NB110-58771IR
FITC	NB110-58771F
HRP	NB110-58771H
Janelia Fluor 525	NB110-58771JF525
Janelia Fluor 549	NB110-58771JF549
Janelia Fluor 646	NB110-58771JF646
Janelia Fluor 669	NB110-58771JF669
PE	NB110-58771PE
PerCP	NB110-58771PCP
mFluor Violet 450 SE	NB110-58771MFV450
mFluor Violet 500 SE	NB110-58771MFV500
mFluor Violet 610 SE	NB110-58771MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-58771SS

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NB100-938

LRRK2 Antibody Pack

1 Pack

NB100-938

Immunohistochemistry: LRRK2 Antibody Pack [NB100-938] - Staining of neurons and glia using NB110-55289 at 2.5ug/ml. Mouse brain, 40x.

Western Blot: LRRK2 Antibody Pack [NB100-938] - Detection of murine LRRK2 in mouse brain lysate using NB110-55289.

Reactivity:

Hu, Mu, Bv, Ce, I, Pl, Hu(-)

Species Glossary

Reactivity: Hu, Mu, Bv, Ce, I, Pl, Hu(-)

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit

Host: Rabbit

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NBP1-78299

LRRK2 Antibody - BSA Free

0.1 ml

NBP1-78299

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78299AF350
Alexa Fluor 405	NBP1-78299AF405
Alexa Fluor 488	NBP1-78299AF488
Alexa Fluor 532	NBP1-78299AF532
Alexa Fluor 594	NBP1-78299AF594
Alexa Fluor 647	NBP1-78299AF647
Alexa Fluor 700	NBP1-78299AF700
Alexa Fluor 750	NBP1-78299AF750
Alexa Fluor Plus 405	NBP1-78299AFP405
Alexa Fluor Plus 488	NBP1-78299AFP488
Alexa Fluor Plus 555	NBP1-78299AFP555
Alexa Fluor Plus 594	NBP1-78299AFP594
Alexa Fluor Plus 647	NBP1-78299AFP647
Alexa Fluor Plus 680	NBP1-78299AFP680
Allophycocyanin	NBP1-78299APC
Biotin	NBP1-78299B
CoraFluor 1	NBP1-78299CL1
DyLight 350	NBP1-78299UV
DyLight 405	NBP1-78299V
DyLight 488	NBP1-78299G
DyLight 550	NBP1-78299R
DyLight 594	NBP1-78299DL594
DyLight 650	NBP1-78299C
DyLight 680	NBP1-78299FR
DyLight 755	NBP1-78299IR
FITC	NBP1-78299F
HRP	NBP1-78299H
Janelia Fluor 525	NBP1-78299JF525
Janelia Fluor 549	NBP1-78299JF549
Janelia Fluor 646	NBP1-78299JF646
Janelia Fluor 669	NBP1-78299JF669
PE	NBP1-78299PE
PerCP	NBP1-78299PCP
mFluor Violet 450 SE	NBP1-78299MFV450
mFluor Violet 500 SE	NBP1-78299MFV500
mFluor Violet 610 SE	NBP1-78299MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78299SS

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NB110-55289

LRRK2 Antibody - BSA Free

0.1 ml

NB110-55289

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-55289AF350
Alexa Fluor 405	NB110-55289AF405
Alexa Fluor 488	NB110-55289AF488
Alexa Fluor 532	NB110-55289AF532
Alexa Fluor 594	NB110-55289AF594
Alexa Fluor 647	NB110-55289AF647
Alexa Fluor 700	NB110-55289AF700
Alexa Fluor 750	NB110-55289AF750
Alexa Fluor Plus 405	NB110-55289AFP405
Alexa Fluor Plus 488	NB110-55289AFP488
Alexa Fluor Plus 555	NB110-55289AFP555
Alexa Fluor Plus 594	NB110-55289AFP594
Alexa Fluor Plus 647	NB110-55289AFP647
Alexa Fluor Plus 680	NB110-55289AFP680
Allophycocyanin	NB110-55289APC
Biotin	NB110-55289B
CoraFluor 1	NB110-55289CL1
DyLight 350	NB110-55289UV
DyLight 405	NB110-55289V
DyLight 488	NB110-55289G
DyLight 550	NB110-55289R
DyLight 594	NB110-55289DL594
DyLight 650	NB110-55289C
DyLight 680	NB110-55289FR
DyLight 755	NB110-55289IR
FITC	NB110-55289F
HRP	NB110-55289H
Janelia Fluor 525	NB110-55289JF525
Janelia Fluor 549	NB110-55289JF549
Janelia Fluor 646	NB110-55289JF646
Janelia Fluor 669	NB110-55289JF669
PE	NB110-55289PE
PerCP	NB110-55289PCP
mFluor Violet 450 SE	NB110-55289MFV450
mFluor Violet 500 SE	NB110-55289MFV500
mFluor Violet 610 SE	NB110-55289MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-55289SS

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NBP2-42175

LRRK2 Antibody (S138-6) - BSA Free

0.1 mg

NBP2-42175

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody (S138-6) [NBP2-42175] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-42175AF350
Alexa Fluor 405	NBP2-42175AF405
Alexa Fluor 488	NBP2-42175AF488
Alexa Fluor 532	NBP2-42175AF532
Alexa Fluor 594	NBP2-42175AF594
Alexa Fluor 647	NBP2-42175AF647
Alexa Fluor 700	NBP2-42175AF700
Alexa Fluor 750	NBP2-42175AF750
Alexa Fluor Plus 405	NBP2-42175AFP405
Alexa Fluor Plus 488	NBP2-42175AFP488
Alexa Fluor Plus 555	NBP2-42175AFP555
Alexa Fluor Plus 594	NBP2-42175AFP594
Alexa Fluor Plus 647	NBP2-42175AFP647
Alexa Fluor Plus 680	NBP2-42175AFP680
Allophycocyanin	NBP2-42175APC
Biotin	NBP2-42175B
CoraFluor 1	NBP2-42175CL1
DyLight 350	NBP2-42175UV
DyLight 405	NBP2-42175V
DyLight 488	NBP2-42175G
DyLight 550	NBP2-42175R
DyLight 594	NBP2-42175DL594
DyLight 650	NBP2-42175C
DyLight 680	NBP2-42175FR
DyLight 755	NBP2-42175IR
FITC	NBP2-42175F
HRP	NBP2-42175H
Janelia Fluor 525	NBP2-42175JF525
Janelia Fluor 549	NBP2-42175JF549
Janelia Fluor 646	NBP2-42175JF646
Janelia Fluor 669	NBP2-42175JF669
PE	NBP2-42175PE
PerCP	NBP2-42175PCP
mFluor Violet 450 SE	NBP2-42175MFV450
mFluor Violet 500 SE	NBP2-42175MFV500
mFluor Violet 610 SE	NBP2-42175MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-42175-0.025mg

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AF6674

CTS008) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm and processes of neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human LRRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6674) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm and processes of neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human LRRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6674) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm and processes of neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6674U-100UG
Alexa Fluor 405	AF6674V-100UG
Alexa Fluor 488	AF6674G-100UG
Alexa Fluor 532	AF6674X-100UG
Alexa Fluor 594	AF6674T-100UG
Alexa Fluor 647	AF6674R-100UG
Alexa Fluor 700	AF6674N-100UG
Alexa Fluor 750	AF6674S-100UG
Alexa Fluor Plus 405	AF6674AFP405-100UG
Alexa Fluor Plus 488	AF6674AFP488-100UG
Alexa Fluor Plus 555	AF6674AFP555-100UG
Alexa Fluor Plus 594	AF6674AFP594-100UG
Alexa Fluor Plus 647	AF6674AFP647-100UG
Alexa Fluor Plus 680	AF6674AFP680-100UG
Allophycocyanin	AF6674A
Biotin	AF6674B
CoraFluor 1	AF6674CL1
DyLight 350	AF6674D
DyLight 405	AF6674E
DyLight 488	AF6674K
DyLight 550	AF6674L
DyLight 594	AF6674M
DyLight 650	AF6674W
DyLight 680	AF6674Y
DyLight 755	AF6674Z
FITC	AF6674F
HRP	AF6674H
Janelia Fluor 525	AF6674JF525
Janelia Fluor 549	AF6674I
Janelia Fluor 646	AF6674J
Janelia Fluor 669	AF6674JF669
PE	AF6674P
PerCP	AF6674C
mFluor Violet 450 SE	AF6674MFV450
mFluor Violet 500 SE	AF6674MFV500
mFluor Violet 610 SE	AF6674MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6674-SP

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NB300-268PEP

LRRK2 Antibody Blocking Peptide

(2 Publications)

0.05 mg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-48524

LRRK2 Antibody - BSA Free

0.1 ml

NBP2-48524

Staining of human cell line A549 shows localization to nucleus & vesicles.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-48524-25ul

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MAB6674

LRRK2 Antibody (521313) [Unconjugated]

100 μg

MAB6674

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human LRRK2 Monoclonal Antibody (Catalog # MAB6674) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human LRRK2 Monoclonal Antibody (Catalog # MAB6674) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6674U-100UG
Alexa Fluor 405	FAB6674V-100UG
Alexa Fluor 488	FAB6674G-100UG
Alexa Fluor 532	FAB6674X-100UG
Alexa Fluor 594	FAB6674T-100UG
Alexa Fluor 647	FAB6674R-100UG
Alexa Fluor 700	FAB6674N-100UG
Alexa Fluor 750	FAB6674S-100UG
Alexa Fluor Plus 405	FAB6674AFP405-100UG
Alexa Fluor Plus 488	FAB6674AFP488-100UG
Alexa Fluor Plus 555	FAB6674AFP555-100UG
Alexa Fluor Plus 594	FAB6674AFP594-100UG
Alexa Fluor Plus 647	FAB6674AFP647-100UG
Alexa Fluor Plus 680	FAB6674AFP680-100UG
Allophycocyanin	FAB6674A
Biotin	FAB6674B
CoraFluor 1	FAB6674CL1
DyLight 350	FAB6674D
DyLight 405	FAB6674E
DyLight 488	FAB6674K
DyLight 550	FAB6674L
DyLight 594	FAB6674M
DyLight 650	FAB6674W
DyLight 680	FAB6674Y
DyLight 755	FAB6674Z
FITC	FAB6674F
HRP	FAB6674H
Janelia Fluor 525	FAB6674JF525
Janelia Fluor 549	FAB6674I
Janelia Fluor 646	FAB6674J
Janelia Fluor 669	FAB6674JF669
PE	FAB6674P
PerCP	FAB6674C
mFluor Violet 450 SE	FAB6674MFV450
mFluor Violet 500 SE	FAB6674MFV500
mFluor Violet 610 SE	FAB6674MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6674-SP

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NBP3-21626

LRRK2 Antibody (SR1449) - BSA Free

100 ul

NBP3-21626

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21626-50ul

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MAB66741

LRRK2 Antibody (521309) [Unconjugated]

100 μg

MAB66741

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LRRK2 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF-7 human breast cancer cell line (negative staining) using Mouse Anti-Human LRRK2 Monoclonal Antibody (Catalog # MAB66741) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="LRRK2 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF-7 human breast cancer cell line (negative staining) using Mouse Anti-Human LRRK2 Monoclonal Antibody (Catalog # MAB66741) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB66741U-100UG
Alexa Fluor 405	FAB66741V-100UG
Alexa Fluor 488	FAB66741G-100UG
Alexa Fluor 532	FAB66741X-100UG
Alexa Fluor 594	FAB66741T-100UG
Alexa Fluor 647	FAB66741R-100UG
Alexa Fluor 700	FAB66741N-100UG
Alexa Fluor 750	FAB66741S-100UG
Alexa Fluor Plus 405	FAB66741AFP405-100UG
Alexa Fluor Plus 488	FAB66741AFP488-100UG
Alexa Fluor Plus 555	FAB66741AFP555-100UG
Alexa Fluor Plus 594	FAB66741AFP594-100UG
Alexa Fluor Plus 647	FAB66741AFP647-100UG
Alexa Fluor Plus 680	FAB66741AFP680-100UG
Allophycocyanin	FAB66741A
Biotin	FAB66741B
CoraFluor 1	FAB66741CL1
DyLight 350	FAB66741D
DyLight 405	FAB66741E
DyLight 488	FAB66741K
DyLight 550	FAB66741L
DyLight 594	FAB66741M
DyLight 650	FAB66741W
DyLight 680	FAB66741Y
DyLight 755	FAB66741Z
FITC	FAB66741F
HRP	FAB66741H
Janelia Fluor 525	FAB66741JF525
Janelia Fluor 549	FAB66741I
Janelia Fluor 646	FAB66741J
Janelia Fluor 669	FAB66741JF669
PE	FAB66741P
PerCP	FAB66741C
mFluor Violet 450 SE	FAB66741MFV450
mFluor Violet 500 SE	FAB66741MFV500
mFluor Violet 610 SE	FAB66741MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB66741-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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LRRK2 Antibody - BSA Free

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