| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6134-SP | |||
HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human Proteinase 3/Myeloblastin/PRTN3 Mono-clonal Antibody (Catalog # MAB6134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6134-SP | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Proteinase 3/ Myeloblastin/PRTN3 was detected in immersion fixed human granulocytes using Mouse Anti-Human Proteinase 3/ Myeloblastin/PRTN3 Monoclonal Antibody (Catalog # MAB61341) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB61341-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22274-50ul | |||
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-NBP1-25966-img0017.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.")
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0020.jpg)
![N/A Proteinase 3/Myeloblastin/PRTN3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Proteinase_3_DY613405_ELISA_2489.jpg "N/A Proteinase 3/Myeloblastin/PRTN3 [Biotin]")
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [H00005657-D01P] - Analysis of PRTN3 expression in mouse brain.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-H00005657-D01P-img0003.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [H00005657-D01P] - Analysis of PRTN3 expression in mouse brain.")
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [H00005657-D01P] - Analysis of PRTN3 expression in transfected 293T cell line by PRTN3 polyclonal antibody.Lane 1: PRTN3 transfected lysate(27.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-H00005657-D01P-img0004.jpg)
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody (SR1444) [NBP3-22274] - Western blot analysis using NBP3-22274 in HL60 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22274_rabbit-proteinase-3-myeloblastin-prtn3-mab-sr1444-57202317215196.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody (SR1444) [NBP3-22274] - Western blot analysis using NBP3-22274 in HL60 cell lysate.")
![SDS-Page: Recombinant Human Proteinase 3/Myeloblastin/PRTN3 Protein [H00005657-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005657-Q01-1.jpg "SDS-Page: Recombinant Human Proteinase 3/Myeloblastin/PRTN3 Protein [H00005657-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Proteinase 3/Myeloblastin/PRTN3 Protein [H00005657-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Proteinase-3-Myeloblastin-PRTN3-Protein-SDS-Page-H00005657-P01-img0003.jpg "SDS-Page: Recombinant Human Proteinase 3/Myeloblastin/PRTN3 Protein [H00005657-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.")
![ELISA: Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (Colorimetric) [NBP3-18227] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18227_human-proteinase-3-myeloblastin-prtn3-elisa-kit-color-4320249115686.jpg)
