Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3587-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1233P | |||
| Carrier Free | MAB1233-SP | |||
| Carrier Free | FAB1233V-100UG | |||
| Carrier Free | FAB1233U-100UG | |||
| Carrier Free | FAB1233T-100UG | |||
| Carrier Free | FAB1233S-100UG | |||
| Carrier Free | FAB1233R-100UG | |||
| Carrier Free | FAB1233N-100UG | |||
| Carrier Free | FAB1233G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1747-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1923SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2360-SP | |||
| Carrier Free | FAB2360V-100UG | |||
| Carrier Free | FAB2360U-100UG | |||
| Carrier Free | FAB2360T-100UG | |||
| Carrier Free | FAB2360S-100UG | |||
| Carrier Free | FAB2360R-100UG | |||
| Carrier Free | FAB2360N-100UG | |||
| Carrier Free | FAB2360G-100UG | |||
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0003.jpg "Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.")
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0006.jpg)
![Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Flow-Cytometry-NBP2-37575-img0004.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0002.jpg)
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human lung cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0005.jpg)
![ELISA: IRAK4 Antibody (2H9) [NBP2-37575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-ELISA-NBP2-37575-img0001.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0009.jpg "Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on PC-12 cell lysates with Rabbit anti-DDB1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0007.jpg)
![Western Blot: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.Positive control:Lane 1: HepG2 Lane 2: NIH-3T3Lane 3: MCF-7 Lane 4: Rat kidney tissue](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Western-Blot-NBP2-75465-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody (JU32-35) [NBP2-75465] - Staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunocytochemistry-Immunofluorescence-NBP2-75465-img0002.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0004.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody (JU32-35) [NBP2-75465] - Analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DDB1-Antibody-JU32-35-Immunohistochemistry-Paraffin-NBP2-75465-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Western-Blot-NBP2-67179-img0006.jpg "Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0005.jpg)
![Flow Cytometry: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Flow-Cytometry-NBP2-67179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0003.jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Rat hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112433..jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Mouse hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112623..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse cerebral cortex tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112828..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse hippocampus tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112950..jpg)
![Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Western-Blot-NBP2-67121-img0009.jpg "Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0008.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Argo2/elF2C2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0012.jpg)
![Flow Cytometry: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Flow-Cytometry-NBP2-67121-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0003.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0004.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0005.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0006.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ago2/elF2C2 antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0010.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Argo/elF2C2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0011.jpg)
![Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Western-Blot-NBP2-67766-img0007.jpg "Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (SD20-92) [NBP2-67766] - Staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunocytochemistry-Immunofluorescence-NBP2-67766-img0005.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded mouse colon tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0017.jpg)
![Flow Cytometry: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Flow-Cytometry-NBP2-67766-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0008.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human prostate carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0009.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human skin tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0010.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0011.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human stomach carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0012.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0014.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human thyroid tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0015.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human small intestine tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0016.jpg)
![Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.](http://images.novusbio.com/fullsize/Sir2.1-Antibody-Western-Blot-NB100-1923-img0003.jpg "Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.")
![Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NB100-93329-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93329-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NB100-93329-img0004.jpg)