Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5565-SP | |||
| Carrier Free | IC5565N-100UG | |||
| Carrier Free | IC5565V-100UG | |||
| Carrier Free | IC5565G-100UG | |||
| Carrier Free | IC5565U-100UG | |||
| Carrier Free | IC5565T-100UG | |||
| Carrier Free | IC5565S-100UG | |||
| Carrier Free | IC5565R-100UG | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2625U-100UG | |||
| Alexa Fluor 405 | FAB2625V-100UG | |||
| Alexa Fluor 488 | FAB2625G-100UG | |||
| Alexa Fluor 532 | FAB2625X-100UG | |||
| Alexa Fluor 594 | FAB2625T-100UG | |||
| Alexa Fluor 647 | FAB2625R-100UG | |||
| Alexa Fluor 700 | FAB2625N-100UG | |||
| Alexa Fluor 750 | FAB2625S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2625-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1936-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB21363-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2896U-100UG | |||
| Alexa Fluor 405 | FAB2896V-100UG | |||
| Alexa Fluor 488 | FAB2896G-100UG | |||
| Alexa Fluor 532 | FAB2896X-100UG | |||
| Alexa Fluor 594 | FAB2896T-100UG | |||
| Alexa Fluor 647 | FAB2896R-100UG | |||
| Alexa Fluor 700 | FAB2896N-100UG | |||
| Alexa Fluor 750 | FAB2896S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2896-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB24601-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB26662-SP | |||
| Carrier Free | FAB26662V-100UG | |||
| Carrier Free | FAB26662U-100UG | |||
| Carrier Free | FAB26662T-100UG | |||
| Carrier Free | FAB26662S-100UG | |||
| Carrier Free | FAB26662R-100UG | |||
| Carrier Free | FAB26662N-100UG | |||
| Carrier Free | FAB26662G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2185-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6036-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2059U-100UG | |||
| Alexa Fluor 405 | FAB2059V-100UG | |||
| Alexa Fluor 488 | FAB2059G-100UG | |||
| Alexa Fluor 532 | FAB2059X-100UG | |||
| Alexa Fluor 594 | FAB2059T-100UG | |||
| Alexa Fluor 647 | FAB2059R-100UG | |||
| Alexa Fluor 700 | FAB2059N-100UG | |||
| Alexa Fluor 750 | FAB2059S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2059-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5018-SP | |||
![Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Western-Blot-NB100-1710-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-1710] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-NB100-1710-img0003.jpg)
