Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7494APC | |||
| FITC | NB7496 | |||
| HRP | NB7497 | |||
| Janelia Fluor 525 | NB7494JF525 | |||
| Janelia Fluor 669 | NB7494JF669 | |||
| PE | NB7494PE | |||
| PerCP | NB7494PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91761SS | |||
![N/A CXCL16 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CXCL16_DY503_ELISA_2073.jpg "N/A CXCL16 [Biotin]")
![N/A CCL1/I-309/TCA-3 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CCL1_DY272_ELISA_1878.jpg "N/A CCL1/I-309/TCA-3 [Biotin]")
![N/A IL-17F [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17F_DY2057_ELISA_1758.jpg "N/A IL-17F [Biotin]")
![N/A YM1/Chitinase 3-like 3 [Biotin]](http://images.novusbio.com/fullsize2/DATA_YM1_DY2446_ELISA_1830.jpg "N/A YM1/Chitinase 3-like 3 [Biotin]")
![N/A S100A8 [Biotin]](http://images.novusbio.com/fullsize2/DATA_S100A8_DY457005_ELISA_2462.jpg "N/A S100A8 [Biotin]")
![N/A CCL8/MCP-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CCL8_DY281_ELISA_1890.jpg "N/A CCL8/MCP-2 [Biotin]")
![N/A vWF-A2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_vWFA2_DY276405_ELISA_2405.jpg "N/A vWF-A2 [Biotin]")
![N/A CCL26/Eotaxin-3 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CCL26_DY346_ELISA_1944.jpg "N/A CCL26/Eotaxin-3 [Biotin]")
![N/A Klotho [Biotin]](http://images.novusbio.com/fullsize2/DATA_Klotho_DY533405_ELISA_2477.jpg "N/A Klotho [Biotin]")
![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0003.jpg "Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")
![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Triton X-100(1%0 Whole cell lysate(20mcg) from Cos7 cells transiently transfected with control vector (v) or an expression vector driving expression of Glu-Glu tagged RalBP1 (a to d).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0001.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).")
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0002.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL based detection.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0006.jpg)